m6A-centered Disease Response Information
General Information of the Disease (ID: M6ADIS0091)
| Name |
Acute ischemic stroke
|
||||
|---|---|---|---|---|---|
| ICD |
ICD-11: 8B11
|
||||
Full List of Target Gene(s) of This m6A-centered Disease Response
Mutated in multiple advanced cancers 1 (PTEN)
| In total 1 item(s) under this target gene | ||||
| Experiment 1 Reporting the m6A-centered Disease Response by This Target Gene | [3] | |||
| Response Summary | YTHDC1 promoted Mutated in multiple advanced cancers 1 (PTEN) mRNA degradation to increase Akt phosphorylation, thus facilitating neuronal survival in particular after ischemia, modulating m6A reader YTHDC1 provide a potential therapeutic target for ischemic stroke. | |||
| Responsed Disease | Acute ischemic stroke [ICD-11: 8B11] | |||
| Target Regulator | YTH domain-containing protein 1 (YTHDC1) | READER | ||
| Target Regulation | Down regulation | |||
| In-vitro Model | HEK293T | Normal | Homo sapiens | CVCL_0063 |
| In-vivo Model | Male SD rats weighing 200-250 g were anesthetized using 4% isoflurane in 70% N2O and 30% O2 with a mask. A midline incision was made in the neck, the left external carotid artery (ECA) was carefully exposed and dissected, a monofilament nylon suture with a diameter of about 0.22 mm was inserted from the ECA into the internal carotid artery, and the left middle cerebral artery (MCA) was blocked. After occlusion for 90 minutes, the suture was removed for reperfusion, and ECA was ligated to close the wound. Sham-operated rats underwent the same surgery except for suture insertion. Rats were maintained on top of a warming pad (RWD, 69003) during the above procedures. The breathing machine was used to monitor the respiration of rats. The rats were returned to a heated cage during the recovery phase with free access to food and water. | |||
microRNA 335 (MIR335)
| In total 1 item(s) under this target gene | ||||
| Experiment 1 Reporting the m6A-centered Disease Response by This Target Gene | [15] | |||
| Response Summary | METTL3-mediated m6A methylation increases the maturation of microRNA 335 (MIR335), which promotes SG formation and reduces the apoptosis level of injury neurons and cells, and provides a potential therapeutic strategy for acute ischemic stroke. | |||
| Responsed Disease | Acute ischemic stroke [ICD-11: 8B11] | |||
| Target Regulator | Methyltransferase-like 3 (METTL3) | WRITER | ||
| Target Regulation | Up regulation | |||
| Cell Process | RNA maturation | |||
| Cell apoptosis | ||||
| In-vitro Model | PC-12 | Lung papillary adenocarcinoma | Homo sapiens | CVCL_S979 |
| In-vivo Model | After a median incision on the neck, the left common carotid artery (CCA), internal carotid artery (ICA), and external carotid artery (ECA) were isolated. The left CCA and the ECA were ligated. | |||
Full List of Crosstalk(s) between m6A Modification and Epigenetic Regulation Related to This Disease
| In total 11 item(s) under this disease | ||
| Crosstalk ID: M6ACROT02073 | ||
| m6A Regulator | ELAV-like protein 1 (ELAVL1) | |
| m6A Target | DNA (cytosine-5)-methyltransferase 3B (DNMT3B) | |
| Epigenetic Regulator | DNA (cytosine-5)-methyltransferase 3B (DNMT3B) | |
| Regulated Target | Serine/threonine-protein kinase PINK1, mitochondrial (PINK1) | |
| Crosstalk relationship | m6A → DNA modification | |
| Crosstalk ID: M6ACROT03206 | ||
| m6A Regulator | Methyltransferase-like 14 (METTL14) | |
| m6A Target | Histone deacetylase 3 (HDAC3) | |
| Epigenetic Regulator | Histone deacetylase 3 (HDAC3) | |
| Crosstalk relationship | m6A → Histone modification | |
| Crosstalk ID: M6ACROT03207 | ||
| m6A Regulator | Insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3) | |
| m6A Target | Histone deacetylase 3 (HDAC3) | |
| Epigenetic Regulator | Histone deacetylase 3 (HDAC3) | |
| Crosstalk relationship | m6A → Histone modification | |
| Crosstalk ID: M6ACROT05025 | ||
| m6A Regulator | YTH domain-containing protein 1 (YTHDC1) | |
| m6A Target | Ubiquitin-like-conjugating enzyme ATG10 (ATG10) | |
| Epigenetic Regulator | Colorectal neoplasia differentially expressed (CRNDE) | |
| Regulated Target | YTH N6-methyladenosine RNA binding protein C1 (YTHDC1) | |
| Crosstalk relationship | ncRNA → m6A | |
| Crosstalk ID: M6ACROT05099 | ||
| m6A Regulator | Fat mass and obesity-associated protein (FTO) | |
| m6A Target | Dynamin-1-like protein (DRP1) | |
| Epigenetic Regulator | ZNFX1 antisense RNA 1 (ZFAS1) | |
| Regulated Target | FTO alpha-ketoglutarate dependent dioxygenase (FTO) | |
| Crosstalk relationship | ncRNA → m6A | |
| Crosstalk ID: M6ACROT05105 | ||
| m6A Regulator | RNA demethylase ALKBH5 (ALKBH5) | |
| m6A Target | H19 imprinted maternally expressed transcript (H19) | |
| Epigenetic Regulator | H19 imprinted maternally expressed transcript (H19) | |
| Regulated Target | hsa-miR-124-3p | |
| Crosstalk relationship | m6A → ncRNA | |
| Crosstalk ID: M6ACROT05263 | ||
| m6A Regulator | YTH domain-containing family protein 1 (YTHDF1) | |
| m6A Target | Transcription factor p65 (RELA) | |
| Epigenetic Regulator | hsa-miR-421-3p | |
| Regulated Target | YTH domain-containing family protein 1 (YTHDF1) | |
| Crosstalk relationship | ncRNA → m6A | |
| Crosstalk ID: M6ACROT05434 | ||
| m6A Regulator | Methyltransferase-like 3 (METTL3) | |
| m6A Target | microRNA 335 (MIR335) | |
| Epigenetic Regulator | MicroRNA 335 (MIR335) | |
| Regulated Target | ZFP36 ring finger protein like 1 (ZFP36L1) | |
| Crosstalk relationship | m6A → ncRNA | |
| Crosstalk ID: M6ACROT05690 | ||
| m6A Regulator | Methyltransferase-like 3 (METTL3) | |
| m6A Target | LINC00035 (ABHD11-AS1) | |
| Epigenetic Regulator | LINC00035 (ABHD11-AS1) | |
| Regulated Target | hsa-miR-1301-3p | |
| Crosstalk relationship | m6A → ncRNA | |
| Crosstalk ID: M6ACROT05727 | ||
| m6A Regulator | Fat mass and obesity-associated protein (FTO) | |
| m6A Target | Maternally expressed 3 (MEG3) | |
| Epigenetic Regulator | Maternally expressed 3 (MEG3) | |
| Crosstalk relationship | m6A → ncRNA | |
| Crosstalk ID: M6ACROT05821 | ||
| m6A Regulator | RNA demethylase ALKBH5 (ALKBH5) | |
| m6A Target | Small nucleolar RNA host gene 3 (SNHG3) | |
| Epigenetic Regulator | Small nucleolar RNA host gene 3 (SNHG3) | |
| Regulated Target | ELAV-like protein 1 (HuR/ELAVL1) | |
| Crosstalk relationship | m6A → ncRNA | |
References