Name	Laryngeal cancer
ICD	ICD-11: 2C23

In total 1 item(s) under this target gene
Experiment 1 Reporting the m6A-centered Disease Response by This Target Gene				[1]
Response Summary	RBM15-mediated m6A modification of TMBIM6 mRNA enhanced TMBIM6 stability through IGF2BP3-dependent. Laryngeal squamous cell cancer cells were transfected with shRBM15 lentivirus for 48h, and the qRT-PCR data indicated that the mRNA levels of CPNE5, TMBIM6, and ATPase family AAA domain-containing protein 3A (ATAD3A) decreased after RBM15 knockdown.
Responsed Disease	Laryngeal cancer [ICD-11: 2C23]
Target Regulator	RNA-binding motif protein 15 (RBM15)		WRITER	Regulator Info
Target Regulation	Up regulation
Cell Process	RNA stability
	Cell apoptosis
In-vitro Model	AMC-HN-8	Laryngeal squamous cell carcinoma	Homo sapiens	CVCL_5966
	NHBEC (Normal human bronchial epithelial cell)
	Tu 177	Laryngeal squamous cell carcinoma	Homo sapiens	CVCL_4913
	Tu 212	Head and neck squamous cell carcinoma	Homo sapiens	CVCL_4915
In-vivo Model	For tumor growth studies, whether in vivo RBM15 knockdown/overexpression experiments or in vivo rescue experiments, each group included six mice. Each mouse was injected with 100 uL of lentivirus-transfected tumor cells.

In total 2 item(s) under this target gene
Experiment 1 Reporting the m6A-centered Disease Response by This Target Gene				[1]
Response Summary	RBM15-mediated m6A modification of Bax inhibitor 1 (TMBIM6) mRNA enhanced TMBIM6 stability through IGF2BP3-dependent. Laryngeal squamous cell cancer cells were transfected with shRBM15 lentivirus for 48h, and the qRT-PCR data indicated that the mRNA levels of CPNE5, TMBIM6, and ATAD3A decreased after RBM15 knockdown.
Responsed Disease	Laryngeal cancer [ICD-11: 2C23]
Target Regulator	Insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3)		READER	Regulator Info
Target Regulation	Up regulation
Cell Process	RNA stability
	Cell apoptosis
In-vitro Model	AMC-HN-8	Laryngeal squamous cell carcinoma	Homo sapiens	CVCL_5966
	NHBEC (Normal human bronchial epithelial cell)
	Tu 177	Laryngeal squamous cell carcinoma	Homo sapiens	CVCL_4913
	Tu 212	Head and neck squamous cell carcinoma	Homo sapiens	CVCL_4915
In-vivo Model	For tumor growth studies, whether in vivo RBM15 knockdown/overexpression experiments or in vivo rescue experiments, each group included six mice. Each mouse was injected with 100 uL of lentivirus-transfected tumor cells.
Experiment 2 Reporting the m6A-centered Disease Response by This Target Gene				[1]
Response Summary	RBM15-mediated m6A modification of Bax inhibitor 1 (TMBIM6) mRNA enhanced TMBIM6 stability through IGF2BP3-dependent. Laryngeal squamous cell cancer cells were transfected with shRBM15 lentivirus for 48h, and the qRT-PCR data indicated that the mRNA levels of CPNE5, TMBIM6, and ATAD3A decreased after RBM15 knockdown.
Responsed Disease	Laryngeal cancer [ICD-11: 2C23]
Target Regulator	RNA-binding motif protein 15 (RBM15)		WRITER	Regulator Info
Target Regulation	Up regulation
Cell Process	RNA stability
	Cell apoptosis
In-vitro Model	AMC-HN-8	Laryngeal squamous cell carcinoma	Homo sapiens	CVCL_5966
	NHBEC (Normal human bronchial epithelial cell)
	Tu 177	Laryngeal squamous cell carcinoma	Homo sapiens	CVCL_4913
	Tu 212	Head and neck squamous cell carcinoma	Homo sapiens	CVCL_4915
In-vivo Model	For tumor growth studies, whether in vivo RBM15 knockdown/overexpression experiments or in vivo rescue experiments, each group included six mice. Each mouse was injected with 100 uL of lentivirus-transfected tumor cells.

In total 1 item(s) under this target gene
Experiment 1 Reporting the m6A-centered Disease Response by This Target Gene				[1]
Response Summary	RBM15-mediated m6A modification of TMBIM6 mRNA enhanced TMBIM6 stability through IGF2BP3-dependent. Laryngeal squamous cell cancer cells were transfected with shRBM15 lentivirus for 48h, and the qRT-PCR data indicated that the mRNA levels of Copine-5 (CPNE5), TMBIM6, and ATAD3A decreased after RBM15 knockdown.
Responsed Disease	Laryngeal cancer [ICD-11: 2C23]
Target Regulator	RNA-binding motif protein 15 (RBM15)		WRITER	Regulator Info
Target Regulation	Up regulation
Cell Process	RNA stability
	Cell apoptosis
In-vitro Model	AMC-HN-8	Laryngeal squamous cell carcinoma	Homo sapiens	CVCL_5966
	NHBEC (Normal human bronchial epithelial cell)
	Tu 177	Laryngeal squamous cell carcinoma	Homo sapiens	CVCL_4913
	Tu 212	Head and neck squamous cell carcinoma	Homo sapiens	CVCL_4915
In-vivo Model	For tumor growth studies, whether in vivo RBM15 knockdown/overexpression experiments or in vivo rescue experiments, each group included six mice. Each mouse was injected with 100 uL of lentivirus-transfected tumor cells.

In total 2 item(s) under this target gene
Experiment 1 Reporting the m6A-centered Disease Response by This Target Gene				[3]
Response Summary	ALKBH5 mediates KCNQ1 opposite strand/antisense transcript 1 (KCNQ1OT1) expression via an m6A-YTHDF2-dependent manner and KCNQ1OT1 could directly bind to HOXA9 to further regulate the proliferation, invasion and metastasis of laryngeal squamous cell carcinoma cells.
Responsed Disease	Laryngeal cancer [ICD-11: 2C23]
Target Regulator	YTH domain-containing family protein 2 (YTHDF2)		READER	Regulator Info
Target Regulation	Up regulation
Cell Process	Cell proliferation and metastasis
In-vitro Model	AMC-HN-8	Laryngeal squamous cell carcinoma	Homo sapiens	CVCL_5966
	HOK	Normal	Hexagrammos otakii	CVCL_YE19
	Tu 212	Head and neck squamous cell carcinoma	Homo sapiens	CVCL_4915
In-vivo Model	1 × 106 (100 ul) cells of infected and uninfected by lentiviral were, respectively, injected subcutaneously into nude mice which divided randomly into scramble group and shKCNQ1OT1-1 group.
Experiment 2 Reporting the m6A-centered Disease Response by This Target Gene				[3]
Response Summary	ALKBH5 mediates KCNQ1 opposite strand/antisense transcript 1 (KCNQ1OT1) expression via an m6A-YTHDF2-dependent manner and KCNQ1OT1 could directly bind to HOXA9 to further regulate the proliferation, invasion and metastasis of laryngeal squamous cell carcinoma cells.
Responsed Disease	Laryngeal cancer [ICD-11: 2C23]
Target Regulator	RNA demethylase ALKBH5 (ALKBH5)		ERASER	Regulator Info
Target Regulation	Up regulation
Cell Process	Cell proliferation and metastasis
In-vitro Model	AMC-HN-8	Laryngeal squamous cell carcinoma	Homo sapiens	CVCL_5966
	HOK	Normal	Hexagrammos otakii	CVCL_YE19
	Tu 212	Head and neck squamous cell carcinoma	Homo sapiens	CVCL_4915
In-vivo Model	1 × 106 (100 ul) cells of infected and uninfected by lentiviral were, respectively, injected subcutaneously into nude mice which divided randomly into scramble group and shKCNQ1OT1-1 group.

Ref 1	RBM15 facilitates laryngeal squamous cell carcinoma progression by regulating TMBIM6 stability through IGF2BP3 dependent. J Exp Clin Cancer Res. 2021 Feb 26;40(1):80. doi: 10.1186/s13046-021-01871-4.
Ref 2	Discovery of the Highly Potent PI3K/mTOR Dual Inhibitor PF-04979064 through Structure-Based Drug Design. ACS Med Chem Lett. 2012 Nov 7;4(1):91-7. doi: 10.1021/ml300309h. eCollection 2013 Jan 10.
Ref 3	ALKBH5-mediated m6A modification of lncRNA KCNQ1OT1 triggers the development of LSCC via upregulation of HOXA9. J Cell Mol Med. 2022 Jan;26(2):385-398. doi: 10.1111/jcmm.17091. Epub 2021 Dec 1.
Ref 4	Ketobemidone is a substrate for cytochrome P4502C9 and 3A4, but not for P-glycoprotein. Xenobiotica. 2005 Aug;35(8):785-96. doi: 10.1080/00498250500183181.
Ref 5	A CXCR4 antagonist CTCE-9908 inhibits primary tumor growth and metastasis of breast cancer. J Surg Res. 2009 Aug;155(2):231-6. doi: 10.1016/j.jss.2008.06.044. Epub 2008 Aug 9.
Ref 6	Cytochrome P450 isoforms catalyze formation of catechol estrogen quinones that react with DNA. Metabolism. 2007 Jul;56(7):887-94. doi: 10.1016/j.metabol.2007.03.001.