m6A-centered Disease Response Information

General Information of the Disease (ID: M6ADIS0027)
Name
Laryngeal cancer
ICD
ICD-11: 2C23
Full List of Target Gene(s) of This m6A-centered Disease Response
ATPase family AAA domain-containing protein 3A (ATAD3A)
 Target Info 
In total 1 item(s) under this target gene
Experiment 1 Reporting the m6A-centered Disease Response by This Target Gene [1]
Response Summary RBM15-mediated m6A modification of TMBIM6 mRNA enhanced TMBIM6 stability through IGF2BP3-dependent. Laryngeal squamous cell cancer cells were transfected with shRBM15 lentivirus for 48h, and the qRT-PCR data indicated that the mRNA levels of CPNE5, TMBIM6, and ATPase family AAA domain-containing protein 3A (ATAD3A) decreased after RBM15 knockdown.
Responsed Disease Laryngeal cancer [ICD-11: 2C23]
Target Regulator RNA-binding motif protein 15 (RBM15) WRITER
 Regulator Info 
Target Regulation Up regulation
Cell Process RNA stability
Cell apoptosis
In-vitro Model AMC-HN-8 Laryngeal squamous cell carcinoma Homo sapiens CVCL_5966
NHBEC (Normal human bronchial epithelial cell)
Tu 177 Laryngeal squamous cell carcinoma Homo sapiens CVCL_4913
Tu 212 Head and neck squamous cell carcinoma Homo sapiens CVCL_4915
In-vivo Model For tumor growth studies, whether in vivo RBM15 knockdown/overexpression experiments or in vivo rescue experiments, each group included six mice. Each mouse was injected with 100 uL of lentivirus-transfected tumor cells.
Bax inhibitor 1 (TMBIM6)
 Target Info 
In total 2 item(s) under this target gene
Experiment 1 Reporting the m6A-centered Disease Response by This Target Gene [1]
Response Summary RBM15-mediated m6A modification of Bax inhibitor 1 (TMBIM6) mRNA enhanced TMBIM6 stability through IGF2BP3-dependent. Laryngeal squamous cell cancer cells were transfected with shRBM15 lentivirus for 48h, and the qRT-PCR data indicated that the mRNA levels of CPNE5, TMBIM6, and ATAD3A decreased after RBM15 knockdown.
Responsed Disease Laryngeal cancer [ICD-11: 2C23]
Target Regulator Insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3) READER
 Regulator Info 
Target Regulation Up regulation
Cell Process RNA stability
Cell apoptosis
In-vitro Model AMC-HN-8 Laryngeal squamous cell carcinoma Homo sapiens CVCL_5966
NHBEC (Normal human bronchial epithelial cell)
Tu 177 Laryngeal squamous cell carcinoma Homo sapiens CVCL_4913
Tu 212 Head and neck squamous cell carcinoma Homo sapiens CVCL_4915
In-vivo Model For tumor growth studies, whether in vivo RBM15 knockdown/overexpression experiments or in vivo rescue experiments, each group included six mice. Each mouse was injected with 100 uL of lentivirus-transfected tumor cells.
Experiment 2 Reporting the m6A-centered Disease Response by This Target Gene [1]
Response Summary RBM15-mediated m6A modification of Bax inhibitor 1 (TMBIM6) mRNA enhanced TMBIM6 stability through IGF2BP3-dependent. Laryngeal squamous cell cancer cells were transfected with shRBM15 lentivirus for 48h, and the qRT-PCR data indicated that the mRNA levels of CPNE5, TMBIM6, and ATAD3A decreased after RBM15 knockdown.
Responsed Disease Laryngeal cancer [ICD-11: 2C23]
Target Regulator RNA-binding motif protein 15 (RBM15) WRITER
 Regulator Info 
Target Regulation Up regulation
Cell Process RNA stability
Cell apoptosis
In-vitro Model AMC-HN-8 Laryngeal squamous cell carcinoma Homo sapiens CVCL_5966
NHBEC (Normal human bronchial epithelial cell)
Tu 177 Laryngeal squamous cell carcinoma Homo sapiens CVCL_4913
Tu 212 Head and neck squamous cell carcinoma Homo sapiens CVCL_4915
In-vivo Model For tumor growth studies, whether in vivo RBM15 knockdown/overexpression experiments or in vivo rescue experiments, each group included six mice. Each mouse was injected with 100 uL of lentivirus-transfected tumor cells.
Copine-5 (CPNE5)
 Target Info 
In total 1 item(s) under this target gene
Experiment 1 Reporting the m6A-centered Disease Response by This Target Gene [1]
Response Summary RBM15-mediated m6A modification of TMBIM6 mRNA enhanced TMBIM6 stability through IGF2BP3-dependent. Laryngeal squamous cell cancer cells were transfected with shRBM15 lentivirus for 48h, and the qRT-PCR data indicated that the mRNA levels of Copine-5 (CPNE5), TMBIM6, and ATAD3A decreased after RBM15 knockdown.
Responsed Disease Laryngeal cancer [ICD-11: 2C23]
Target Regulator RNA-binding motif protein 15 (RBM15) WRITER
 Regulator Info 
Target Regulation Up regulation
Cell Process RNA stability
Cell apoptosis
In-vitro Model AMC-HN-8 Laryngeal squamous cell carcinoma Homo sapiens CVCL_5966
NHBEC (Normal human bronchial epithelial cell)
Tu 177 Laryngeal squamous cell carcinoma Homo sapiens CVCL_4913
Tu 212 Head and neck squamous cell carcinoma Homo sapiens CVCL_4915
In-vivo Model For tumor growth studies, whether in vivo RBM15 knockdown/overexpression experiments or in vivo rescue experiments, each group included six mice. Each mouse was injected with 100 uL of lentivirus-transfected tumor cells.
KCNQ1 opposite strand/antisense transcript 1 (KCNQ1OT1)
 Target Info 
In total 2 item(s) under this target gene
Experiment 1 Reporting the m6A-centered Disease Response by This Target Gene [2]
Response Summary ALKBH5 mediates KCNQ1 opposite strand/antisense transcript 1 (KCNQ1OT1) expression via an m6A-YTHDF2-dependent manner and KCNQ1OT1 could directly bind to HOXA9 to further regulate the proliferation, invasion and metastasis of laryngeal squamous cell carcinoma cells.
Responsed Disease Laryngeal cancer [ICD-11: 2C23]
Target Regulator YTH domain-containing family protein 2 (YTHDF2) READER
 Regulator Info 
Target Regulation Up regulation
Cell Process Cell proliferation and metastasis
In-vitro Model AMC-HN-8 Laryngeal squamous cell carcinoma Homo sapiens CVCL_5966
HOK Normal Hexagrammos otakii CVCL_YE19
Tu 212 Head and neck squamous cell carcinoma Homo sapiens CVCL_4915
In-vivo Model 1 × 106 (100 ul) cells of infected and uninfected by lentiviral were, respectively, injected subcutaneously into nude mice which divided randomly into scramble group and shKCNQ1OT1-1 group.
Experiment 2 Reporting the m6A-centered Disease Response by This Target Gene [2]
Response Summary ALKBH5 mediates KCNQ1 opposite strand/antisense transcript 1 (KCNQ1OT1) expression via an m6A-YTHDF2-dependent manner and KCNQ1OT1 could directly bind to HOXA9 to further regulate the proliferation, invasion and metastasis of laryngeal squamous cell carcinoma cells.
Responsed Disease Laryngeal cancer [ICD-11: 2C23]
Target Regulator RNA demethylase ALKBH5 (ALKBH5) ERASER
 Regulator Info 
Target Regulation Up regulation
Cell Process Cell proliferation and metastasis
In-vitro Model AMC-HN-8 Laryngeal squamous cell carcinoma Homo sapiens CVCL_5966
HOK Normal Hexagrammos otakii CVCL_YE19
Tu 212 Head and neck squamous cell carcinoma Homo sapiens CVCL_4915
In-vivo Model 1 × 106 (100 ul) cells of infected and uninfected by lentiviral were, respectively, injected subcutaneously into nude mice which divided randomly into scramble group and shKCNQ1OT1-1 group.
References
Ref 1 RBM15 facilitates laryngeal squamous cell carcinoma progression by regulating TMBIM6 stability through IGF2BP3 dependent. J Exp Clin Cancer Res. 2021 Feb 26;40(1):80. doi: 10.1186/s13046-021-01871-4.
Ref 2 ALKBH5-mediated m6A modification of lncRNA KCNQ1OT1 triggers the development of LSCC via upregulation of HOXA9. J Cell Mol Med. 2022 Jan;26(2):385-398. doi: 10.1111/jcmm.17091. Epub 2021 Dec 1.