General Information of the m6A Target Gene (ID: M6ATAR00651)
Target Name hsa-miR-34a
Synonyms
hsa-mir-34a; MIRN34A
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Gene Name hsa-miR-34a
Chromosomal Location 1p36.22
Family MicroRNAs
Gene ID 407040
HGNC ID
HGNC:31635
miRBase ID
MI0000268
Full List of m6A Methylation Regulator of This Target Gene and Corresponding Disease/Drug Response(s)
hsa-miR-34a can be regulated by the following regulator(s), and cause disease/drug response(s). You can browse detail information of regulator(s) or disease/drug response(s).
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Methyltransferase-like 3 (METTL3) [WRITER]
In total 1 item(s) under this regulator
Experiment 1 Reporting the m6A Methylation Regulator of This Target Gene [2]
Response Summary METTL3/m6A-mediated hsa-miR-34a maturation in AAA formation and provide a novel therapeutic target and diagnostic biomarker for AAA treatment.
Target Regulation Up regulation
Responsed Disease Abdominal aortic aneurysm ICD-11: BD50.4
In-vitro Model VSMC (Human aortic vascular smooth muscle cells)
In-vivo Model Male C57BL/6J mice were anesthetized with an intraperitoneal injection of pentobarbital (40 mg/kg). The abdominal aorta between the renal arteries and the bifurcation of the iliac arteries was disassociated from the surrounding structures. Video microscopy was used to assay the diameter of the aorta in triplicate. After the measurements were taken, a small piece of gauze dipped in 0.5 mol/L CaCl2 was spread perivascularly onto the aortic passage for 15 min. Control mice received substitute treatment with NaCl (0.9%)-soaked gauze for 15 min. Then, the aorta was rinsed with 0.9% sterile saline, and the incision was sutured. After 3 or 6 weeks, all the animals were sacrificed, and the aortas were harvested for further analysis.
Aortic aneurysm or dissection [ICD-11: BD50]
In total 1 item(s) under this disease
Experiment 1 Reporting the m6A-centered Disease Response [2]
Response Summary METTL3/m6A-mediated hsa-miR-34a maturation in AAA formation and provide a novel therapeutic target and diagnostic biomarker for AAA treatment.
Responsed Disease Abdominal aortic aneurysm [ICD-11: BD50.4]
Target Regulator Methyltransferase-like 3 (METTL3) WRITER
Target Regulation Up regulation
In-vitro Model VSMC (Human aortic vascular smooth muscle cells)
In-vivo Model Male C57BL/6J mice were anesthetized with an intraperitoneal injection of pentobarbital (40 mg/kg). The abdominal aorta between the renal arteries and the bifurcation of the iliac arteries was disassociated from the surrounding structures. Video microscopy was used to assay the diameter of the aorta in triplicate. After the measurements were taken, a small piece of gauze dipped in 0.5 mol/L CaCl2 was spread perivascularly onto the aortic passage for 15 min. Control mice received substitute treatment with NaCl (0.9%)-soaked gauze for 15 min. Then, the aorta was rinsed with 0.9% sterile saline, and the incision was sutured. After 3 or 6 weeks, all the animals were sacrificed, and the aortas were harvested for further analysis.
Full List of Crosstalk(s) between m6A Modification and Epigenetic Regulation Related to This Regulator
RNA modification
m6A Regulator: Methyltransferase-like 14 (METTL14)
In total 13 item(s) under this m6A regulator
Crosstalk ID: M6ACROT00611
Epigenetic Regulator Interferon-inducible protein 4 (ADAR1)
Regulated Target G1/S-specific cyclin-E1 (CCNE1)
Crosstalk relationship m6A → A-to-I
Crosstalk ID: M6ACROT00612
Epigenetic Regulator Y-box-binding protein 1 (YBX1)
Regulated Target G1/S-specific cyclin-E1 (CCNE1)
Crosstalk relationship m6A → m5C
Crosstalk ID: M6ACROT00613
Epigenetic Regulator Methylcytosine dioxygenase TET1 (TET1)
Regulated Target G1/S-specific cyclin-E1 (CCNE1)
Crosstalk relationship m6A → m5C
Crosstalk ID: M6ACROT00614
Epigenetic Regulator Interferon-inducible protein 4 (ADAR1)
Regulated Target Nuclear paraspeckle assembly transcript 1 (NEAT1)
Crosstalk relationship A-to-I → m6A
Crosstalk ID: M6ACROT00615
Epigenetic Regulator Y-box-binding protein 1 (YBX1)
Regulated Target Zinc finger protein SNAI1 (SNAI1)
Crosstalk relationship m6A → m5C
Crosstalk ID: M6ACROT00616
Epigenetic Regulator Methylcytosine dioxygenase TET3 (TET3)
Regulated Target Apoptosis regulator Bcl-2 (BCL2)
Crosstalk relationship m6A → m5C
Crosstalk ID: M6ACROT00617
Epigenetic Regulator Y-box-binding protein 1 (YBX1)
Regulated Target Apoptosis regulator Bcl-2 (BCL2)
Crosstalk relationship m6A → m5C
Crosstalk ID: M6ACROT00618
Epigenetic Regulator Fat mass and obesity-associated protein (FTO)
Regulated Target Apoptosis regulator Bcl-2 (BCL2)
Crosstalk relationship m6A → m6Am
Crosstalk ID: M6ACROT00619
Epigenetic Regulator Pseudouridylate synthase 1 homolog (PUS1)
Regulated Target Apoptosis regulator Bcl-2 (BCL2)
Crosstalk relationship m6A → Pseudouridine
Crosstalk ID: M6ACROT00620
Epigenetic Regulator THO complex subunit 4 (ALYREF)
Regulated Target N-myc proto-oncogene protein (MYCN)
Crosstalk relationship m6A → m5C
Crosstalk ID: M6ACROT00621
Epigenetic Regulator Fat mass and obesity-associated protein (FTO)
Regulated Target Cytochrome c, somatic (CYCS)
Crosstalk relationship m6A → m6Am
Crosstalk ID: M6ACROT00622
Epigenetic Regulator Fat mass and obesity-associated protein (FTO)
Regulated Target NAD-dependent protein deacetylase sirtuin-1 (SIRT1)
Crosstalk relationship m6A → m6Am
Crosstalk ID: M6ACROT00623
Epigenetic Regulator Fat mass and obesity-associated protein (FTO)
Regulated Target Autophagy protein 5 (ATG5)
Crosstalk relationship m6A → m6Am
Non-coding RNA
m6A Regulator: Methyltransferase-like 3 (METTL3)
In total 1 item(s) under this m6A regulator
Crosstalk ID: M6ACROT05607
Epigenetic Regulator hsa-miR-34a
Regulated Target NAD-dependent protein deacetylase sirtuin-1 (SIRT1)
Crosstalk relationship m6A → ncRNA
Disease Abdominal aortic aneurysm
m6A Regulator: Methyltransferase-like 14 (METTL14)
In total 1 item(s) under this m6A regulator
Crosstalk ID: M6ACROT05804
Epigenetic Regulator MicroRNA 34a (MIR34A)
Regulated Target NAD-dependent protein deacetylase sirtuin-1 (SIRT1)
Crosstalk relationship m6A → ncRNA
References
Ref 1 Phase 2 randomized study of p53 antisense oligonucleotide (cenersen) plus idarubicin with or without cytarabine in refractory and relapsed acute myeloid leukemia. Cancer. 2012 Jan 15;118(2):418-27. doi: 10.1002/cncr.26292. Epub 2011 Jun 29.
Ref 2 METTL3 Induces AAA Development and Progression by Modulating N6-Methyladenosine-Dependent Primary miR34a Processing. Mol Ther Nucleic Acids. 2020 Sep 4;21:394-411. doi: 10.1016/j.omtn.2020.06.005. Epub 2020 Jun 10.