m6A-centered Crosstalk Information

Mechanism of Crosstalk between m6A Modification and Epigenetic Regulation

m6A Modification:
Crosstalk ID	M6ACROT00620				[1], [2], [3]
m⁶A modification hsa-miR-34a hsa-miR-34a METTL14 Methylation : m⁶A sites Indirect Enhancement RNA modification MYCN MYCN ALYREF : modification sites
m6A Regulator	Methyltransferase-like 14 (METTL14)			WRITER	Regulator Info
m6A Target	hsa-miR-34a				Targert Gene
Epigenetic Regulation that have Cross-talk with This m6A Modification:
Epigenetic Regulation Type	RNA modification (RNAMod) >> 5-methylcytidine (m5C)
Epigenetic Regulator	THO complex subunit 4 (ALYREF)			READER	View Details
Regulated Target	N-myc proto-oncogene protein (MYCN)				View Details
Crosstalk Relationship	m6A → m5C			Enhancement
Crosstalk Mechanism	m6A modification indirectly impacts RNA modification through downstream signaling pathways
Crosstalk Summary	METTL14 interacts with hsa-miR-34a, reading its m6A level and promoting its physical interaction with N-myc proto-oncogene protein (MYCN), which was regulated by ALYREF-mediated m5C modification.
In-vitro Model	Kelly	Neuroblastoma	Homo sapiens		CVCL_2092
In-vitro Model	SK-N-BE(2)-C	Neuroblastoma	Homo sapiens		CVCL_0529

References

Ref 1	An ALYREF-MYCN coactivator complex drives neuroblastoma tumorigenesis through effects on USP3 and MYCN stability. Nat Commun. 2021 Mar 25;12(1):1881. doi: 10.1038/s41467-021-22143-x.
Ref 2	N6-Methyladenosine Induced miR-34a-5p Promotes TNF-alpha-Induced Nucleus Pulposus Cell Senescence by Targeting SIRT1. Front Cell Dev Biol. 2021 Mar 5;9:642437. doi: 10.3389/fcell.2021.642437. eCollection 2021.
Ref 3	Structural Insights on Tiny Peptide Nucleic Acid (PNA) Analogues of miRNA-34a: An in silico and Experimental Integrated Approach. Front Chem. 2020 Nov 23;8:568575. doi: 10.3389/fchem.2020.568575. eCollection 2020.