m6A Target Gene Information

General Information of the m6A Target Gene (ID: M6ATAR00258)
Target Name Forkhead box protein M1 (FOXM1)
Synonyms
Forkhead-related protein FKHL16; Hepatocyte nuclear factor 3 forkhead homolog 11; HFH-11; HNF-3/fork-head homolog 11; M-phase phosphoprotein 2; MPM-2 reactive phosphoprotein 2; Transcription factor Trident; Winged-helix factor from INS-1 cells; FKHL16; HFH11; MPP2; WIN
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Gene Name FOXM1
Chromosomal Location 12p13.33
Function
Transcription factor regulating the expression of cell cycle genes essential for DNA replication and mitosis. Plays a role in the control of cell proliferation. Also plays a role in DNA break repair, participating in the DNA damage checkpoint response. Promotes transcription of PHB2.
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Gene ID 2305
Uniprot ID
FOXM1_HUMAN
HGNC ID
HGNC:3818
Ensembl Gene ID
ENSG00000111206
KEGG ID
hsa:2305
Full List of m6A Methylation Regulator of This Target Gene and Corresponding Disease/Drug Response(s)
FOXM1 can be regulated by the following regulator(s), and cause disease/drug response(s). You can browse detail information of regulator(s) or disease/drug response(s).
Browse Regulator
Browse Disease
RNA demethylase ALKBH5 (ALKBH5) [ERASER]
 Regulator Info 
Representative RNA-seq result indicating the expression of this target gene regulated by ALKBH5
Cell Line HaCAT cell line Homo sapiens
Treatment: siALKBH5 HaCAT cells
Control: siControl HaCAT cells
 GSE211076
Regulation
logFC: 1.11E+00
p-value: 1.78E-33
More Results Click to View More RNA-seq Results
In total 4 item(s) under this regulator
Experiment 1 Reporting the m6A Methylation Regulator of This Target Gene [1]
Response Summary ALKBH5 and FOXM1-AS disrupted GSC tumorigenesis through the Forkhead box protein M1 (FOXM1) axis.
Target Regulation Up regulation
Responsed Disease Glioblastoma ICD-11: 2A00.00
 Disease Info 
Pathway Response Glioma hsa05214
Cell Process Cells proliferation
Signaling pathways regulating pluripotency of stem cells (hsa04550)
In-vitro Model LN-229 Glioblastoma Homo sapiens CVCL_0393
Hs 683 Oligodendroglioma Homo sapiens CVCL_0844
SW1783 Anaplastic astrocytoma Homo sapiens CVCL_1722
U-87MG ATCC Glioblastoma Homo sapiens CVCL_0022
In-vivo Model For the animal survival analysis, mice were intracranially injected with 10,000 GSCs and maintained until moribund or 80 days after injection. For the rescue studies, GSCs with ALKBH5 or FOXM1-AS shRNAs were co-transfected with a FOXM1, ALKBH5 wild-type or mutant expression construct.
Experiment 2 Reporting the m6A Methylation Regulator of This Target Gene [2]
Response Summary m6A demethylase ALKBH5 affects the proliferation and invasion of lung adenocarcinoma cells under IH by downregulating m6A modification on Forkhead box protein M1 (FOXM1) mRNA and by promoting FOXM1 expression.high FOXM1 expression was associated with cisplatin-based chemotherapy resistance and poor prognosis
Target Regulation Up regulation
Responsed Disease Lung cancer ICD-11: 2C25
 Disease Info 
Pathway Response Cellular senescence hsa04218
Cell Process Cell proliferation and invasion
In-vitro Model A-549 Lung adenocarcinoma Homo sapiens CVCL_0023
NCI-H522 Lung adenocarcinoma Homo sapiens CVCL_1567
In-vivo Model 1 × 107 A549 cells were subcutaneously implanted into 4-week-old NOD/SCID mice.
Experiment 3 Reporting the m6A Methylation Regulator of This Target Gene [3]
Response Summary AKLBH5-induced m6A demethylation of Forkhead box protein M1 (FOXM1) mRNA promotes uveal melanoma progression.
Target Regulation Up regulation
Responsed Disease Melanoma of uvea ICD-11: 2D0Y
 Disease Info 
Cell Process Cell proliferation
Cell migration
Cell invasion
Cell apoptosis
In-vitro Model ARPE-19 Normal Homo sapiens CVCL_0145
C918 Uveal melanoma Homo sapiens CVCL_8471
MuM-2B Uveal melanoma Homo sapiens CVCL_3447
In-vivo Model The in vivo experiment method for transplantation of tumors was subcutaneous injection of 1 × 107 ALKBH5-stable knockdown C918 cells into BALB/c nude mice.
Experiment 4 Reporting the m6A Methylation Regulator of This Target Gene [4]
Response Summary ALKBH5 promotes silica-induced lung fibrosis via the miR-320a-3p/FOXM1 axis or targeting Forkhead box protein M1 (FOXM1) directly.
Target Regulation Up regulation
Responsed Disease Pulmonary Fibrosis ICD-11: CB03.4
 Disease Info 
In-vitro Model NIH 3T3 Normal Mus musculus CVCL_0594
MRC-5 Normal Homo sapiens CVCL_0440
In-vivo Model For the mouse model of miR-320a-3p overexpression, a total of 24 male C57BL/6 mice were divided randomly into four groups (n = 6 in each group): saline, silica, silica plus AAV9-miR-NC, and silica plus AAV9-miR-320a-3p. The mice in the silica plus AAV9-miR-NC/AAV9-miR-320a-3p groups were anesthetized using the same method, then administered intratracheally 50 uL AAV9-miR-NC/AAV9-miR-320a-3p per mouse at a titer of 8 × 1012 v. g./ml. Three weeks later, these mice were treated in the same way using anesthesia, saline, and silica as mentioned above. Subsequently, after 4 weeks, the mice were sacrificed, and the lungs were isolated and frozen at -80 ℃ for further study.
Wilms tumor 1-associating protein (WTAP) [WRITER]
 Regulator Info 
Representative RNA-seq result indicating the expression of this target gene regulated by WTAP
Cell Line mouse embryonic stem cells Mus musculus
Treatment: WTAP-/- ESCs
Control: Wild type ESCs
 GSE145309
Regulation
logFC: -9.85E-01
p-value: 1.39E-67
More Results Click to View More RNA-seq Results
In total 1 item(s) under this regulator
Experiment 1 Reporting the m6A Methylation Regulator of This Target Gene [5]
Response Summary A remarkable m6A-modified site was found on the 3'-UTR of FOXD2-AS1, and m6A methyltransferase WTAP promoted the methylation modification, thus enhancing the stability of FOXD2-AS1 transcripts. m6A-modified FOXD2-AS1 accelerates the osteosarcoma progression through m6A manner, which provides new concepts for osteosarcoma tumorigenesis. FOXD2-AS1 interacted with downstream target FOXM1 mRNA through m6A sites, forming a FOXD2-AS1/m6A/Forkhead box protein M1 (FOXM1) complex to heighten FOXM1 mRNA stability.
Target Regulation Up regulation
Responsed Disease Osteosarcoma ICD-11: 2B51
 Disease Info 
In-vitro Model SaOS-2 Osteosarcoma Homo sapiens CVCL_0548
MG-63 Osteosarcoma Homo sapiens CVCL_0426
hFOB 1.19 Normal Homo sapiens CVCL_3708
In-vivo Model In vivo animal assay, FOXD2-AS1 overexpression promoted the tumor growth in mice subcutaneous injection
YTH domain-containing family protein 1 (YTHDF1) [READER]
 Regulator Info 
Representative RNA-seq result indicating the expression of this target gene regulated by YTHDF1
Cell Line AGS cell line Homo sapiens
Treatment: shYTHDF1 AGS
Control: shNC AGS
 GSE166972
Regulation
logFC: -8.94E-01
p-value: 2.29E-02
More Results Click to View More RNA-seq Results
In total 1 item(s) under this regulator
Experiment 1 Reporting the m6A Methylation Regulator of This Target Gene [6]
Response Summary Forkhead box protein M1 (FOXM1) is a target of YTHDF1. Through recognizing and binding to the m6A-modified mRNA of FOXM1, YTHDF1 accelerated the translation process of FOXM1 and promoted breast cancer metastasis.
Target Regulation Up regulation
Responsed Disease Breast cancer ICD-11: 2C60
 Disease Info 
Cell Process Epithelial-mesenchymal transformation
Cell proliferation
Cell invasion
In-vitro Model T-47D Invasive breast carcinoma Homo sapiens CVCL_0553
SK-BR-3 Breast adenocarcinoma Homo sapiens CVCL_0033
MDA-MB-231 Breast adenocarcinoma Homo sapiens CVCL_0062
MCF-7 Invasive breast carcinoma Homo sapiens CVCL_0031
MCF-10A Normal Homo sapiens CVCL_0598
BT-549 Invasive breast carcinoma Homo sapiens CVCL_1092
In-vivo Model NOD/SCID immune-deficient mice were purchased from Shanghai Experimental Animal Center. 2 * 106 MCF-7 cells transduced with sh-NC or sh-YTHDF1-were subcutaneously injected into the mice (5/group). Tumor width and length were measured every 7 days. Tumor volume?=?(length * width2)/2. After 7 weeks, mice were sacrificed, and the weight of tumors was detected. Xenografts were collected for HE staining, immunohistochemistry staining and western blot analysis.For spontaneous lung metastasis assay, 4 * 106 sh-NC or sh-YTHDF1#2 transduced MCF-7 cells were injected into the mammary fat pads of the NOD/SCID mice (5/group). The primary tumor was removed when its volume reached 150 mm3. The mice were sacrificed, and lung metastasis nodules were counted 12 weeks after the removal.
YTH domain-containing family protein 3 (YTHDF3) [READER]
 Regulator Info 
Representative RIP-seq result supporting the interaction between FOXM1 and the regulator
Cell Line Hela Homo sapiens
Regulation logFC: 1.19E+00 GSE86214
In total 1 item(s) under this regulator
Experiment 1 Reporting the m6A Methylation Regulator of This Target Gene [7]
Response Summary m6A reader Ythdf3 protects hematopoietic stem cell integrity under stress by promoting the translation of Forkhead box protein M1 (FOXM1) and Asxl1 transcripts.
Target Regulation Up regulation
Responsed Disease Neoplasms of haematopoietic or lymphoid tissues ICD-11: 2B3Z
 Disease Info 
Pathway Response mRNA surveillance pathway hsa03015), RNA degradation
Cell Process RNA stability
Brain cancer [ICD-11: 2A00]
 Disease Info 
In total 1 item(s) under this disease
Experiment 1 Reporting the m6A-centered Disease Response [1]
Response Summary ALKBH5 and FOXM1-AS disrupted GSC tumorigenesis through the Forkhead box protein M1 (FOXM1) axis.
Responsed Disease Glioblastoma [ICD-11: 2A00.00]
Target Regulator RNA demethylase ALKBH5 (ALKBH5) ERASER
 Regulator Info 
Target Regulation Up regulation
Pathway Response Glioma hsa05214
Cell Process Cells proliferation
Signaling pathways regulating pluripotency of stem cells (hsa04550)
In-vitro Model LN-229 Glioblastoma Homo sapiens CVCL_0393
Hs 683 Oligodendroglioma Homo sapiens CVCL_0844
SW1783 Anaplastic astrocytoma Homo sapiens CVCL_1722
U-87MG ATCC Glioblastoma Homo sapiens CVCL_0022
In-vivo Model For the animal survival analysis, mice were intracranially injected with 10,000 GSCs and maintained until moribund or 80 days after injection. For the rescue studies, GSCs with ALKBH5 or FOXM1-AS shRNAs were co-transfected with a FOXM1, ALKBH5 wild-type or mutant expression construct.
Neoplasms of haematopoietic or lymphoid tissues [ICD-11: 2B3Z]
 Disease Info 
In total 1 item(s) under this disease
Experiment 1 Reporting the m6A-centered Disease Response [7]
Response Summary m6A reader Ythdf3 protects hematopoietic stem cell integrity under stress by promoting the translation of Forkhead box protein M1 (FOXM1) and Asxl1 transcripts.
Responsed Disease Neoplasms of haematopoietic or lymphoid tissues [ICD-11: 2B3Z]
Target Regulator YTH domain-containing family protein 3 (YTHDF3) READER
 Regulator Info 
Target Regulation Up regulation
Pathway Response mRNA surveillance pathway hsa03015), RNA degradation
Cell Process RNA stability
Osteosarcoma [ICD-11: 2B51]
 Disease Info 
In total 1 item(s) under this disease
Experiment 1 Reporting the m6A-centered Disease Response [5]
Response Summary A remarkable m6A-modified site was found on the 3'-UTR of FOXD2-AS1, and m6A methyltransferase WTAP promoted the methylation modification, thus enhancing the stability of FOXD2-AS1 transcripts. m6A-modified FOXD2-AS1 accelerates the osteosarcoma progression through m6A manner, which provides new concepts for osteosarcoma tumorigenesis. FOXD2-AS1 interacted with downstream target FOXM1 mRNA through m6A sites, forming a FOXD2-AS1/m6A/Forkhead box protein M1 (FOXM1) complex to heighten FOXM1 mRNA stability.
Responsed Disease Osteosarcoma [ICD-11: 2B51]
Target Regulator Wilms tumor 1-associating protein (WTAP) WRITER
 Regulator Info 
Target Regulation Up regulation
In-vitro Model SaOS-2 Osteosarcoma Homo sapiens CVCL_0548
MG-63 Osteosarcoma Homo sapiens CVCL_0426
hFOB 1.19 Normal Homo sapiens CVCL_3708
In-vivo Model In vivo animal assay, FOXD2-AS1 overexpression promoted the tumor growth in mice subcutaneous injection
Lung cancer [ICD-11: 2C25]
 Disease Info 
In total 1 item(s) under this disease
Experiment 1 Reporting the m6A-centered Disease Response [2]
Response Summary m6A demethylase ALKBH5 affects the proliferation and invasion of lung adenocarcinoma cells under IH by downregulating m6A modification on Forkhead box protein M1 (FOXM1) mRNA and by promoting FOXM1 expression.high FOXM1 expression was associated with cisplatin-based chemotherapy resistance and poor prognosis
Responsed Disease Lung cancer [ICD-11: 2C25]
Target Regulator RNA demethylase ALKBH5 (ALKBH5) ERASER
 Regulator Info 
Target Regulation Up regulation
Pathway Response Cellular senescence hsa04218
Cell Process Cell proliferation and invasion
In-vitro Model A-549 Lung adenocarcinoma Homo sapiens CVCL_0023
NCI-H522 Lung adenocarcinoma Homo sapiens CVCL_1567
In-vivo Model 1 × 107 A549 cells were subcutaneously implanted into 4-week-old NOD/SCID mice.
Breast cancer [ICD-11: 2C60]
 Disease Info 
In total 1 item(s) under this disease
Experiment 1 Reporting the m6A-centered Disease Response [6]
Response Summary Forkhead box protein M1 (FOXM1) is a target of YTHDF1. Through recognizing and binding to the m6A-modified mRNA of FOXM1, YTHDF1 accelerated the translation process of FOXM1 and promoted breast cancer metastasis.
Responsed Disease Breast cancer [ICD-11: 2C60]
Target Regulator YTH domain-containing family protein 1 (YTHDF1) READER
 Regulator Info 
Target Regulation Up regulation
Cell Process Epithelial-mesenchymal transformation
Cell proliferation
Cell invasion
In-vitro Model T-47D Invasive breast carcinoma Homo sapiens CVCL_0553
SK-BR-3 Breast adenocarcinoma Homo sapiens CVCL_0033
MDA-MB-231 Breast adenocarcinoma Homo sapiens CVCL_0062
MCF-7 Invasive breast carcinoma Homo sapiens CVCL_0031
MCF-10A Normal Homo sapiens CVCL_0598
BT-549 Invasive breast carcinoma Homo sapiens CVCL_1092
In-vivo Model NOD/SCID immune-deficient mice were purchased from Shanghai Experimental Animal Center. 2 * 106 MCF-7 cells transduced with sh-NC or sh-YTHDF1-were subcutaneously injected into the mice (5/group). Tumor width and length were measured every 7 days. Tumor volume?=?(length * width2)/2. After 7 weeks, mice were sacrificed, and the weight of tumors was detected. Xenografts were collected for HE staining, immunohistochemistry staining and western blot analysis.For spontaneous lung metastasis assay, 4 * 106 sh-NC or sh-YTHDF1#2 transduced MCF-7 cells were injected into the mammary fat pads of the NOD/SCID mice (5/group). The primary tumor was removed when its volume reached 150 mm3. The mice were sacrificed, and lung metastasis nodules were counted 12 weeks after the removal.
Melanoma of uvea [ICD-11: 2D0Y]
 Disease Info 
In total 1 item(s) under this disease
Experiment 1 Reporting the m6A-centered Disease Response [3]
Response Summary AKLBH5-induced m6A demethylation of Forkhead box protein M1 (FOXM1) mRNA promotes uveal melanoma progression.
Responsed Disease Melanoma of uvea [ICD-11: 2D0Y]
Target Regulator RNA demethylase ALKBH5 (ALKBH5) ERASER
 Regulator Info 
Target Regulation Up regulation
Cell Process Cell proliferation
Cell migration
Cell invasion
Cell apoptosis
In-vitro Model ARPE-19 Normal Homo sapiens CVCL_0145
C918 Uveal melanoma Homo sapiens CVCL_8471
MuM-2B Uveal melanoma Homo sapiens CVCL_3447
In-vivo Model The in vivo experiment method for transplantation of tumors was subcutaneous injection of 1 × 107 ALKBH5-stable knockdown C918 cells into BALB/c nude mice.
Idiopathic interstitial pneumonitis [ICD-11: CB03]
 Disease Info 
In total 1 item(s) under this disease
Experiment 1 Reporting the m6A-centered Disease Response [4]
Response Summary ALKBH5 promotes silica-induced lung fibrosis via the miR-320a-3p/FOXM1 axis or targeting Forkhead box protein M1 (FOXM1) directly.
Responsed Disease Pulmonary Fibrosis [ICD-11: CB03.4]
Target Regulator RNA demethylase ALKBH5 (ALKBH5) ERASER
 Regulator Info 
Target Regulation Up regulation
In-vitro Model NIH 3T3 Normal Mus musculus CVCL_0594
MRC-5 Normal Homo sapiens CVCL_0440
In-vivo Model For the mouse model of miR-320a-3p overexpression, a total of 24 male C57BL/6 mice were divided randomly into four groups (n = 6 in each group): saline, silica, silica plus AAV9-miR-NC, and silica plus AAV9-miR-320a-3p. The mice in the silica plus AAV9-miR-NC/AAV9-miR-320a-3p groups were anesthetized using the same method, then administered intratracheally 50 uL AAV9-miR-NC/AAV9-miR-320a-3p per mouse at a titer of 8 × 1012 v. g./ml. Three weeks later, these mice were treated in the same way using anesthesia, saline, and silica as mentioned above. Subsequently, after 4 weeks, the mice were sacrificed, and the lungs were isolated and frozen at -80 ℃ for further study.
References
Ref 1 m(6)A Demethylase ALKBH5 Maintains Tumorigenicity of Glioblastoma Stem-like Cells by Sustaining FOXM1 Expression and Cell Proliferation Program. Cancer Cell. 2017 Apr 10;31(4):591-606.e6. doi: 10.1016/j.ccell.2017.02.013. Epub 2017 Mar 23.
Ref 2 ALKBH5-m(6)A-FOXM1 signaling axis promotes proliferation and invasion of lung adenocarcinoma cells under intermittent hypoxia. Biochem Biophys Res Commun. 2020 Jan 8;521(2):499-506. doi: 10.1016/j.bbrc.2019.10.145. Epub 2019 Oct 31.
Ref 3 ALKBH5-mediated m(6)A demethylation of FOXM1 mRNA promotes progression of uveal melanoma. Aging (Albany NY). 2021 Jan 10;13(3):4045-4062. doi: 10.18632/aging.202371. Epub 2021 Jan 10.
Ref 4 ALKBH5 promotes lung fibroblast activation and silica-induced pulmonary fibrosis through miR-320a-3p and FOXM1. Cell Mol Biol Lett. 2022 Mar 12;27(1):26. doi: 10.1186/s11658-022-00329-5.
Ref 5 N(6)-methyladenosine methyltransferase WTAP-stabilized FOXD2-AS1 promotes the osteosarcoma progression through m(6)A/FOXM1 axis. Bioengineered. 2022 Apr;13(4):7963-7973. doi: 10.1080/21655979.2021.2008218.
Ref 6 YTHDF1 promotes breast cancer progression by facilitating FOXM1 translation in an m6A-dependent manner. Cell Biosci. 2022 Feb 23;12(1):19. doi: 10.1186/s13578-022-00759-w.
Ref 7 m(6)A reader Ythdf3 protects hematopoietic stem cell integrity under stress by promoting the translation of Foxm1 and Asxl1 transcripts. Haematologica. 2022 Aug 1;107(8):1922-1927. doi: 10.3324/haematol.2021.279300.