m6A Target Gene Information

General Information of the m6A Target Gene (ID: M6ATAR00140)
Target Name Nuclear paraspeckle assembly transcript 1 (NEAT1)
Synonyms
NEAT1; NCRNA00084; non-protein coding RNA 84; nuclear paraspeckle assembly transcript 1 (non-protein coding); TncRNA; MENepsilon/beta; LINC00084; VINC; trophoblast derived non-protein coding RNA; nuclear enriched abundant transcript 1; long intergenic non-protein coding RNA 84; virus inducible non-coding RNA
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Gene Name NEAT1
Chromosomal Location 11q13.1
Family MicroRNA non-coding host genes; Long non-coding RNAs with non-systematic symbols
Gene ID 283131
HGNC ID
HGNC:30815
Ensembl Gene ID
ENSG00000245532
Full List of m6A Methylation Regulator of This Target Gene and Corresponding Disease/Drug Response(s)
NEAT1 can be regulated by the following regulator(s), and cause disease/drug response(s). You can browse detail information of regulator(s) or disease/drug response(s).
Browse Regulator
Browse Disease
Methyltransferase-like 14 (METTL14) [WRITER]
 Regulator Info 
Representative RNA-seq result indicating the expression of this target gene regulated by METTL14
Cell Line MDA-MB-231 Homo sapiens
Treatment: siMETTL14 MDA-MB-231 cells
Control: MDA-MB-231 cells
 GSE81164
Regulation
logFC: -8.94E-01
p-value: 3.90E-11
More Results Click to View More RNA-seq Results
In total 1 item(s) under this regulator
Experiment 1 Reporting the m6A Methylation Regulator of This Target Gene [1]
Response Summary In renal cell carcinoma, YTHDF2 accelerated the degradation of Nuclear paraspeckle assembly transcript 1 (NEAT1)_1 by selectively recognizing METTL14-mediated m6A marks on Nuclear paraspeckle assembly transcript 1 (NEAT1)_1.
Target Regulation Down regulation
Responsed Disease Renal cell carcinoma ICD-11: 2C90
 Disease Info 
Cell Process Cell proliferation and metastasis
In-vitro Model 769-P Renal cell carcinoma Homo sapiens CVCL_1050
786-O Renal cell carcinoma Homo sapiens CVCL_1051
HK2 Normal Acipenser baerii CVCL_YE28
In-vivo Model Mouse subcutaneous xenograft and lung metastasis experiments were carried out with six 4-week-old male BALB/c nude mice.
Methyltransferase-like 3 (METTL3) [WRITER]
 Regulator Info 
Representative RNA-seq result indicating the expression of this target gene regulated by METTL3
Cell Line LX2 cell line Homo sapiens
Treatment: shMETTL3 LX2 cells
Control: shLuc LX2 cells
 GSE207909
Regulation
logFC: 1.39E+00
p-value: 3.32E-29
More Results Click to View More RNA-seq Results
In total 1 item(s) under this regulator
Experiment 1 Reporting the m6A Methylation Regulator of This Target Gene [2]
Response Summary METTL3-mediated m6A modification induced the aberrant expression of Nuclear paraspeckle assembly transcript 1 (NEAT1) in chronic myelocytic leukemia. Overexpression of NEAT1 inhibited cell viability and promoted the apoptosis of chronic myelocytic leukemia cells. miR-766-5p was upregulated in CML PBMCs and abrogated the effects of NEAT1 on cell viability and apoptosis of the chronic myelocytic leukemia cells.
Target Regulation Up regulation
Responsed Disease Chronic myeloid leukaemia ICD-11: 2B33.2
 Disease Info 
Cell Process Cell viability and apoptosis
In-vitro Model BV-173 Chronic myelogenous leukemia Homo sapiens CVCL_0181
K-562 Chronic myelogenous leukemia Homo sapiens CVCL_0004
KCL-22 Chronic myelogenous leukemia Homo sapiens CVCL_2091
MEG-01 Chronic myelogenous leukemia Homo sapiens CVCL_0425
RNA demethylase ALKBH5 (ALKBH5) [ERASER]
 Regulator Info 
Representative RNA-seq result indicating the expression of this target gene regulated by ALKBH5
Cell Line HaCAT cell line Homo sapiens
Treatment: siALKBH5 HaCAT cells
Control: siControl HaCAT cells
 GSE211076
Regulation
logFC: -6.88E-01
p-value: 8.40E-87
More Results Click to View More RNA-seq Results
In total 5 item(s) under this regulator
Experiment 1 Reporting the m6A Methylation Regulator of This Target Gene [3]
Response Summary Hypoxia-induced ALKBH5 erased m6A deposition from the lncRNA NEAT1, stabilizing the transcript and facilitating Nuclear paraspeckle assembly transcript 1 (NEAT1)-mediated paraspeckle assembly. Ectopic expression of CXCL8 in ALKBH5-deficient glioblastoma multiforme cells partially restored TAM recruitment and tumor progression.
Target Regulation Down regulation
Responsed Disease Glioblastoma ICD-11: 2A00.00
 Disease Info 
Pathway Response Transcriptional misregulation in cancer hsa05202
Cell Process Immune
In-vitro Model GL261 Mouse glioblastoma Mus musculus CVCL_Y003
U87 (A primary glioblastoma cell line)
In-vivo Model Male C57BL/6 mice (4-6 weeks old) were used in all tumor allografting experiments and transplanted with GL261-luc cells (1×105) into the frontal lobes of brains.
Experiment 2 Reporting the m6A Methylation Regulator of This Target Gene [4]
Response Summary ALKBH5 promotes Gastric cancer invasion and metastasis by demethylating the lncRNA Nuclear paraspeckle assembly transcript 1 (NEAT1).
Target Regulation Up regulation
Responsed Disease Gastric cancer ICD-11: 2B72
 Disease Info 
Cell Process Cell proliferation and metastasis
In-vitro Model BGC-823 Gastric carcinoma Homo sapiens CVCL_3360
GES-1 Normal Homo sapiens CVCL_EQ22
SGC-7901 Gastric carcinoma Homo sapiens CVCL_0520
Experiment 3 Reporting the m6A Methylation Regulator of This Target Gene [5]
Response Summary ALKBH5 knockdown suppressed malignant behavior of colon cancer partially through Nuclear paraspeckle assembly transcript 1 (NEAT1) by demethylation in vitro and vivo, suggesting that ALKBH5-NEAT1 axis is a potential therapeutic target for colon cancer treatment.
Target Regulation Up regulation
Responsed Disease Colorectal cancer ICD-11: 2B91
 Disease Info 
Cell Process Cell proliferation and migration
Cell apoptosis
In-vitro Model HCT 8 Colon adenocarcinoma Homo sapiens CVCL_2478
HIEC (Normal intestinal epithelial cells)
LoVo Colon adenocarcinoma Homo sapiens CVCL_0399
RKO Colon carcinoma Homo sapiens CVCL_0504
SW620 Colon adenocarcinoma Homo sapiens CVCL_0547
In-vivo Model To establish xenograft model, RKO cells (1 × 107) tansduced with si-control (si-NC) or si-ALKBH5 or si-ALKBH5+NEAT1 were injected subcutaneously into the right flank of the nude mice (n = 6 each group) every 5 days for 4 times.
Experiment 4 Reporting the m6A Methylation Regulator of This Target Gene [6]
Response Summary ALKBH5 could up-regulate Nuclear paraspeckle assembly transcript 1 (NEAT1) expression by inhibiting m6A enrichment. ALKBH5-induced NEAT1 promoted cell proliferation and migration of HCC by sponging miR-214 in vitro, which provided a potential therapeutic target for HCC.
Target Regulation Up regulation
Responsed Disease Hepatocellular carcinoma ICD-11: 2C12.02
 Disease Info 
Cell Process Cell proliferation and migration
Cell apoptosis
In-vitro Model SMMC-7721 Endocervical adenocarcinoma Homo sapiens CVCL_0534
L-02 Endocervical adenocarcinoma Homo sapiens CVCL_6926
Huh-7 Adult hepatocellular carcinoma Homo sapiens CVCL_0336
Experiment 5 Reporting the m6A Methylation Regulator of This Target Gene [7]
Response Summary ALKBH5 knockdown suppressed cell proliferation, migration and invasion of infantile hemangioma cells, while promoting cell apoptosis. ALKBH5 promoted lncRNA Nuclear paraspeckle assembly transcript 1 (NEAT1) expression by reducing the m6A modification of lncRNA NEAT1.
Target Regulation Up regulation
Responsed Disease Infantile hemangioma ICD-11: 2E+81
 Disease Info 
Cell Process Cell proliferation
Cell migration
Cell invasion
Cell apoptosis
In-vitro Model XPTS-1 Infantile hemangioma Homo sapiens CVCL_9U60
HEK293T Normal Homo sapiens CVCL_0063
YTH domain-containing family protein 2 (YTHDF2) [READER]
 Regulator Info 
In total 1 item(s) under this regulator
Experiment 1 Reporting the m6A Methylation Regulator of This Target Gene [1]
Response Summary In renal cell carcinoma, YTHDF2 accelerated the degradation of Nuclear paraspeckle assembly transcript 1 (NEAT1)_1 by selectively recognizing METTL14-mediated m6A marks on Nuclear paraspeckle assembly transcript 1 (NEAT1)_1.
Target Regulation Down regulation
Responsed Disease Renal cell carcinoma ICD-11: 2C90
 Disease Info 
Cell Process Cell proliferation and metastasis
In-vitro Model 769-P Renal cell carcinoma Homo sapiens CVCL_1050
786-O Renal cell carcinoma Homo sapiens CVCL_1051
HK2 Normal Acipenser baerii CVCL_YE28
In-vivo Model Mouse subcutaneous xenograft and lung metastasis experiments were carried out with six 4-week-old male BALB/c nude mice.
Brain cancer [ICD-11: 2A00]
 Disease Info 
In total 1 item(s) under this disease
Experiment 1 Reporting the m6A-centered Disease Response [3]
Response Summary Hypoxia-induced ALKBH5 erased m6A deposition from the lncRNA NEAT1, stabilizing the transcript and facilitating Nuclear paraspeckle assembly transcript 1 (NEAT1)-mediated paraspeckle assembly. Ectopic expression of CXCL8 in ALKBH5-deficient glioblastoma multiforme cells partially restored TAM recruitment and tumor progression.
Responsed Disease Glioblastoma [ICD-11: 2A00.00]
Target Regulator RNA demethylase ALKBH5 (ALKBH5) ERASER
 Regulator Info 
Target Regulation Down regulation
Pathway Response Transcriptional misregulation in cancer hsa05202
Cell Process Immune
In-vitro Model GL261 Mouse glioblastoma Mus musculus CVCL_Y003
U87 (A primary glioblastoma cell line)
In-vivo Model Male C57BL/6 mice (4-6 weeks old) were used in all tumor allografting experiments and transplanted with GL261-luc cells (1×105) into the frontal lobes of brains.
Malignant haematopoietic neoplasm [ICD-11: 2B33]
 Disease Info 
In total 1 item(s) under this disease
Experiment 1 Reporting the m6A-centered Disease Response [2]
Response Summary METTL3-mediated m6A modification induced the aberrant expression of Nuclear paraspeckle assembly transcript 1 (NEAT1) in chronic myelocytic leukemia. Overexpression of NEAT1 inhibited cell viability and promoted the apoptosis of chronic myelocytic leukemia cells. miR-766-5p was upregulated in CML PBMCs and abrogated the effects of NEAT1 on cell viability and apoptosis of the chronic myelocytic leukemia cells.
Responsed Disease Chronic myeloid leukaemia [ICD-11: 2B33.2]
Target Regulator Methyltransferase-like 3 (METTL3) WRITER
 Regulator Info 
Target Regulation Up regulation
Cell Process Cell viability and apoptosis
In-vitro Model BV-173 Chronic myelogenous leukemia Homo sapiens CVCL_0181
K-562 Chronic myelogenous leukemia Homo sapiens CVCL_0004
KCL-22 Chronic myelogenous leukemia Homo sapiens CVCL_2091
MEG-01 Chronic myelogenous leukemia Homo sapiens CVCL_0425
Gastric cancer [ICD-11: 2B72]
 Disease Info 
In total 1 item(s) under this disease
Experiment 1 Reporting the m6A-centered Disease Response [4]
Response Summary ALKBH5 promotes Gastric cancer invasion and metastasis by demethylating the lncRNA Nuclear paraspeckle assembly transcript 1 (NEAT1).
Responsed Disease Gastric cancer [ICD-11: 2B72]
Target Regulator RNA demethylase ALKBH5 (ALKBH5) ERASER
 Regulator Info 
Target Regulation Up regulation
Cell Process Cell proliferation and metastasis
In-vitro Model BGC-823 Gastric carcinoma Homo sapiens CVCL_3360
GES-1 Normal Homo sapiens CVCL_EQ22
SGC-7901 Gastric carcinoma Homo sapiens CVCL_0520
Colorectal cancer [ICD-11: 2B91]
 Disease Info 
In total 1 item(s) under this disease
Experiment 1 Reporting the m6A-centered Disease Response [5]
Response Summary ALKBH5 knockdown suppressed malignant behavior of colon cancer partially through Nuclear paraspeckle assembly transcript 1 (NEAT1) by demethylation in vitro and vivo, suggesting that ALKBH5-NEAT1 axis is a potential therapeutic target for colon cancer treatment.
Responsed Disease Colorectal cancer [ICD-11: 2B91]
Target Regulator RNA demethylase ALKBH5 (ALKBH5) ERASER
 Regulator Info 
Target Regulation Up regulation
Cell Process Cell proliferation and migration
Cell apoptosis
In-vitro Model HCT 8 Colon adenocarcinoma Homo sapiens CVCL_2478
HIEC (Normal intestinal epithelial cells)
LoVo Colon adenocarcinoma Homo sapiens CVCL_0399
RKO Colon carcinoma Homo sapiens CVCL_0504
SW620 Colon adenocarcinoma Homo sapiens CVCL_0547
In-vivo Model To establish xenograft model, RKO cells (1 × 107) tansduced with si-control (si-NC) or si-ALKBH5 or si-ALKBH5+NEAT1 were injected subcutaneously into the right flank of the nude mice (n = 6 each group) every 5 days for 4 times.
Liver cancer [ICD-11: 2C12]
 Disease Info 
In total 1 item(s) under this disease
Experiment 1 Reporting the m6A-centered Disease Response [6]
Response Summary ALKBH5 could up-regulate Nuclear paraspeckle assembly transcript 1 (NEAT1) expression by inhibiting m6A enrichment. ALKBH5-induced NEAT1 promoted cell proliferation and migration of HCC by sponging miR-214 in vitro, which provided a potential therapeutic target for HCC.
Responsed Disease Hepatocellular carcinoma [ICD-11: 2C12.02]
Target Regulator RNA demethylase ALKBH5 (ALKBH5) ERASER
 Regulator Info 
Target Regulation Up regulation
Cell Process Cell proliferation and migration
Cell apoptosis
In-vitro Model SMMC-7721 Endocervical adenocarcinoma Homo sapiens CVCL_0534
L-02 Endocervical adenocarcinoma Homo sapiens CVCL_6926
Huh-7 Adult hepatocellular carcinoma Homo sapiens CVCL_0336
Renal cell carcinoma [ICD-11: 2C90]
 Disease Info 
In total 2 item(s) under this disease
Experiment 1 Reporting the m6A-centered Disease Response [1]
Response Summary In renal cell carcinoma, YTHDF2 accelerated the degradation of Nuclear paraspeckle assembly transcript 1 (NEAT1)_1 by selectively recognizing METTL14-mediated m6A marks on Nuclear paraspeckle assembly transcript 1 (NEAT1)_1.
Responsed Disease Renal cell carcinoma [ICD-11: 2C90]
Target Regulator Methyltransferase-like 14 (METTL14) WRITER
 Regulator Info 
Target Regulation Down regulation
Cell Process Cell proliferation and metastasis
In-vitro Model 769-P Renal cell carcinoma Homo sapiens CVCL_1050
786-O Renal cell carcinoma Homo sapiens CVCL_1051
HK2 Normal Acipenser baerii CVCL_YE28
In-vivo Model Mouse subcutaneous xenograft and lung metastasis experiments were carried out with six 4-week-old male BALB/c nude mice.
Experiment 2 Reporting the m6A-centered Disease Response [1]
Response Summary In renal cell carcinoma, YTHDF2 accelerated the degradation of Nuclear paraspeckle assembly transcript 1 (NEAT1)_1 by selectively recognizing METTL14-mediated m6A marks on Nuclear paraspeckle assembly transcript 1 (NEAT1)_1.
Responsed Disease Renal cell carcinoma [ICD-11: 2C90]
Target Regulator YTH domain-containing family protein 2 (YTHDF2) READER
 Regulator Info 
Target Regulation Down regulation
Cell Process Cell proliferation and metastasis
In-vitro Model 769-P Renal cell carcinoma Homo sapiens CVCL_1050
786-O Renal cell carcinoma Homo sapiens CVCL_1051
HK2 Normal Acipenser baerii CVCL_YE28
In-vivo Model Mouse subcutaneous xenograft and lung metastasis experiments were carried out with six 4-week-old male BALB/c nude mice.
Infantile hemangioma [ICD-11: 2E81]
 Disease Info 
In total 1 item(s) under this disease
Experiment 1 Reporting the m6A-centered Disease Response [7]
Response Summary ALKBH5 knockdown suppressed cell proliferation, migration and invasion of infantile hemangioma cells, while promoting cell apoptosis. ALKBH5 promoted lncRNA Nuclear paraspeckle assembly transcript 1 (NEAT1) expression by reducing the m6A modification of lncRNA NEAT1.
Responsed Disease Infantile hemangioma [ICD-11: 2E+81]
Target Regulator RNA demethylase ALKBH5 (ALKBH5) ERASER
 Regulator Info 
Target Regulation Up regulation
Cell Process Cell proliferation
Cell migration
Cell invasion
Cell apoptosis
In-vitro Model XPTS-1 Infantile hemangioma Homo sapiens CVCL_9U60
HEK293T Normal Homo sapiens CVCL_0063
References
Ref 1 Methyltransferase-like 14 suppresses growth and metastasis of renal cell carcinoma by decreasing long noncoding RNA NEAT1. Cancer Sci. 2022 Feb;113(2):446-458. doi: 10.1111/cas.15212. Epub 2021 Dec 16.
Ref 2 m(6)A Modification of lncRNA NEAT1 Regulates Chronic Myelocytic Leukemia Progression via miR-766-5p/CDKN1A Axis. Front Oncol. 2021 Jul 20;11:679634. doi: 10.3389/fonc.2021.679634. eCollection 2021.
Ref 3 ALKBH5 Facilitates Hypoxia-Induced Paraspeckle Assembly and IL8 Secretion to Generate an Immunosuppressive Tumor Microenvironment. Cancer Res. 2021 Dec 1;81(23):5876-5888. doi: 10.1158/0008-5472.CAN-21-1456. Epub 2021 Oct 20.
Ref 4 ALKBH5 promotes invasion and metastasis of gastric cancer by decreasing methylation of the lncRNA NEAT1. J Physiol Biochem. 2019 Aug;75(3):379-389. doi: 10.1007/s13105-019-00690-8. Epub 2019 Jul 9.
Ref 5 ALKBH5 promotes colon cancer progression by decreasing methylation of the lncRNA NEAT1. Am J Transl Res. 2020 Aug 15;12(8):4542-4549. eCollection 2020.
Ref 6 LncRNA NEAT1 sponges miR-214 to promoted tumor growth in hepatocellular carcinoma. Mamm Genome. 2022 Mar 31. doi: 10.1007/s00335-022-09952-1. Online ahead of print.
Ref 7 ALKBH5 promotes the progression of infantile hemangioma through regulating the NEAT1/miR-378b/FOSL1 axis. Mol Cell Biochem. 2022 May;477(5):1527-1540. doi: 10.1007/s11010-022-04388-2. Epub 2022 Feb 18.