m6A Target Gene Information

General Information of the m6A Target Gene (ID: M6ATAR01580)

Target Name	Phosphoenolpyruvate carboxykinase [GTP], mitochondrial (PCK2)
Synonyms	PEPCK-M; EC 4.1.1.32; Phosphoenolpyruvate carboxykinase 2, mitochondrial; mtPCK2 Click to Show/Hide
Gene Name	PCK2
Chromosomal Location	14q11.2-q12
Family	Phosphoenolpyruvate carboxykinase [GTP] family
Function	Mitochondrial phosphoenolpyruvate carboxykinase that catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle (PubMed:28955899). Can play an active role in glyceroneogenesis and gluconeogenesis (PubMed:28955899). Click to Show/Hide
Gene ID	5106
Uniprot ID	PCKGM_HUMAN
HGNC ID	HGNC:8725
KEGG ID	hsa:5106

Full List of Crosstalk(s) between m6A Modification and Epigenetic Regulation Related to This Regulator

DNA modification

m6A Regulator: Methyltransferase-like 3 (METTL3)

Regulator Info

In total 1 item(s) under this m6A regulator
Crosstalk ID: M6ACROT02113		Crosstalk Info
Epigenetic Regulator	Methylcytosine dioxygenase TET2 (TET2)
Regulated Target	Phosphoenolpyruvate carboxykinase [GTP], mitochondrial (PCK2)
Crosstalk relationship	DNA modification → m6A
Disease	Hepatic inflammation	Disease Info

m6A Regulator: Methyltransferase-like 14 (METTL14)

Regulator Info

In total 1 item(s) under this m6A regulator
Crosstalk ID: M6ACROT02114		Crosstalk Info
Epigenetic Regulator	Methylcytosine dioxygenase TET2 (TET2)
Regulated Target	Phosphoenolpyruvate carboxykinase [GTP], mitochondrial (PCK2)
Crosstalk relationship	DNA modification → m6A
Disease	Hepatic inflammation	Disease Info

m6A Regulator: Insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1)

Regulator Info

In total 1 item(s) under this m6A regulator
Crosstalk ID: M6ACROT02115		Crosstalk Info
Epigenetic Regulator	Methylcytosine dioxygenase TET2 (TET2)
Regulated Target	Phosphoenolpyruvate carboxykinase [GTP], mitochondrial (PCK2)
Crosstalk relationship	DNA modification → m6A
Disease	Hepatic inflammation	Disease Info

RNA Modification Sequencing Data Associated with the Target (ID: M6ATAR01580)

Phosphoenolpyruvate carboxykinase [GTP], mitochondrial (PCK2)

N4-acetylcytidine (ac4C)

In total 1 m6A sequence/site(s) in this target gene
mod ID: AC4SITE000189		Click to Show/Hide the Full List
mod site	chr14:24103721-24103722:+		[2]
Sequence	TGCTAGACTGGATCTGCCGGCGGTTAGAGGGGGAGGACAGT
Cell/Tissue List	H1
Seq Type List	ac4C-seq
Transcript ID List	ENST00000545054.6; ENST00000559250.5; ENST00000559171.1; ENST00000216780.8; ENST00000557969.1; ENST00000561286.5; ENST00000558096.5
External Link	RMBase: ac4C_site_529

5-methylcytidine (m5C)

In total 1 m6A sequence/site(s) in this target gene
mod ID: M5CSITE000221		Click to Show/Hide the Full List
mod site	chr14:24099121-24099122:+		[3]
Sequence	GTGCCCGACCAGCGGGAGATCATCTCCTTCGGCAGCGGCTA
Seq Type List	Bisulfite-seq
Transcript ID List	ENST00000545054.6; ENST00000560736.5; ENST00000561286.5; ENST00000559250.5; ENST00000558674.5; ENST00000559503.1; ENST00000561050.5; ENST00000396973.8; ENST00000216780.8; ENST00000558096.5
External Link	RMBase: m5C_site_12588

N6-methyladenosine (m6A)

In total 27 m6A sequence/site(s) in this target gene
mod ID: M6ASITE019102		Click to Show/Hide the Full List
mod site	chr14:24097078-24097079:+		[4]
Sequence	CTGAGGCTGAGAATACTGCCACACTGACCCTGCTGGAGCAG
Motif Score	2.053113095
Cell/Tissue List	hESC-HEK293T
Seq Type List	MAZTER-seq
Transcript ID List	ENST00000559250.5; ENST00000560106.5; ENST00000561050.5; ENST00000558096.5; ENST00000216780.8; ENST00000396973.8; ENST00000545054.6; ENST00000561286.5; ENST00000558674.5; ENST00000560736.5; ENST00000559584.5; ENST00000559837.5
External Link	RMBase: m6A_site_241087
mod ID: M6ASITE019103		Click to Show/Hide the Full List
mod site	chr14:24097127-24097128:+		[4]
Sequence	CATCCGAAAGCTCCCCAAGTACAATAACTGGTAAGCCTTGG
Motif Score	2.856142857
Cell/Tissue List	hESC-HEK293T
Seq Type List	MAZTER-seq
Transcript ID List	ENST00000216780.8; ENST00000560106.5; ENST00000561050.5; ENST00000558096.5; ENST00000545054.6; ENST00000560736.5; ENST00000559250.5; ENST00000559584.5; ENST00000396973.8; ENST00000561286.5; ENST00000559837.5; ENST00000558674.5
External Link	RMBase: m6A_site_241088
mod ID: M6ASITE019104		Click to Show/Hide the Full List
mod site	chr14:24098215-24098216:+		[5]
Sequence	CCCCCAGCTGGCTGGCCCGCACAGACCCCAAGGATGTGGCA
Motif Score	2.830589286
Cell/Tissue List	HEK293
Seq Type List	m6A-REF-seq
Transcript ID List	ENST00000561286.5; ENST00000396973.8; ENST00000560736.5; ENST00000560106.5; ENST00000545054.6; ENST00000559584.5; ENST00000561050.5; ENST00000559250.5; ENST00000558096.5; ENST00000559837.5; ENST00000216780.8; ENST00000558674.5
External Link	RMBase: m6A_site_241089
mod ID: M6ASITE019105		Click to Show/Hide the Full List
mod site	chr14:24098279-24098280:+		[5]
Sequence	TGTAACTCCTTCTCAGCGGGACACGGTACCACTCCCGCCTG
Motif Score	3.643047619
Cell/Tissue List	kidney; hESC-HEK293T
Seq Type List	m6A-REF-seq; MAZTER-seq
Transcript ID List	ENST00000396973.8; ENST00000216780.8; ENST00000558674.5; ENST00000558096.5; ENST00000559837.5; ENST00000545054.6; ENST00000561050.5; ENST00000560736.5; ENST00000560106.5; ENST00000561286.5; ENST00000559250.5
External Link	RMBase: m6A_site_241090
mod ID: M6ASITE019106		Click to Show/Hide the Full List
mod site	chr14:24098599-24098600:+		[4]
Sequence	GTATTATGACCCGACTGGGGACACCTGTGCTTCAGGCCCTG
Motif Score	3.643047619
Cell/Tissue List	hESC-HEK293T
Seq Type List	MAZTER-seq
Transcript ID List	ENST00000561050.5; ENST00000558096.5; ENST00000396973.8; ENST00000545054.6; ENST00000559837.5; ENST00000559250.5; ENST00000216780.8; ENST00000558674.5; ENST00000559503.1; ENST00000560736.5; ENST00000561286.5
External Link	RMBase: m6A_site_241091
mod ID: M6ASITE019107		Click to Show/Hide the Full List
mod site	chr14:24098673-24098674:+		[4]
Sequence	GTGGGCCAGCCCCTGACAGGACAAGGTAAGCACCTGCTCTG
Motif Score	3.643047619
Cell/Tissue List	hESC-HEK293T
Seq Type List	MAZTER-seq
Transcript ID List	ENST00000558674.5; ENST00000559837.5; ENST00000559503.1; ENST00000561286.5; ENST00000396973.8; ENST00000545054.6; ENST00000561050.5; ENST00000559250.5; ENST00000216780.8; ENST00000560736.5; ENST00000558096.5
External Link	RMBase: m6A_site_241092
mod ID: M6ASITE019108		Click to Show/Hide the Full List
mod site	chr14:24099228-24099229:+		[5]
Sequence	TGAGGGCTGGCTGGCAGAGCACATGCTGGTGAGGGCCTGGT
Motif Score	2.830589286
Cell/Tissue List	kidney; liver
Seq Type List	m6A-REF-seq
Transcript ID List	ENST00000558674.5; ENST00000558096.5; ENST00000561286.5; ENST00000561050.5; ENST00000559250.5; ENST00000396973.8; ENST00000559503.1; ENST00000545054.6; ENST00000216780.8
External Link	RMBase: m6A_site_241093
mod ID: M6ASITE019109		Click to Show/Hide the Full List
mod site	chr14:24100080-24100081:+		[4]
Sequence	CCAATCCCAACGCCATGGCTACAATCCAGAGTAACACTATT
Motif Score	2.078666667
Cell/Tissue List	hESC-HEK293T
Seq Type List	MAZTER-seq
Transcript ID List	ENST00000545054.6; ENST00000396973.8; ENST00000561286.5; ENST00000561050.5; ENST00000559250.5; ENST00000216780.8; ENST00000558674.5; ENST00000559503.1; ENST00000558096.5
External Link	RMBase: m6A_site_241094
mod ID: M6ASITE019110		Click to Show/Hide the Full List
mod site	chr14:24102755-24102756:+		[4]
Sequence	TGTATTTCCTCTGCCAGGTGACAAGGAGCCCTGTGCACATC
Motif Score	2.859755952
Cell/Tissue List	hESC-HEK293T
Seq Type List	MAZTER-seq
Transcript ID List	ENST00000557969.1; ENST00000561286.5; ENST00000216780.8; ENST00000545054.6; ENST00000558096.5; ENST00000559250.5; ENST00000559503.1; ENST00000559171.1
External Link	RMBase: m6A_site_241095
mod ID: M6ASITE019111		Click to Show/Hide the Full List
mod site	chr14:24102779-24102780:+		[6]
Sequence	GGAGCCCTGTGCACATCCCAACTCTCGATTTTGTGCCCCGG
Motif Score	2.595904762
Cell/Tissue List	HEK293T
Seq Type List	DART-seq
Transcript ID List	ENST00000559250.5; ENST00000558096.5; ENST00000557969.1; ENST00000561286.5; ENST00000559171.1; ENST00000545054.6; ENST00000216780.8; ENST00000559503.1
External Link	RMBase: m6A_site_241096
mod ID: M6ASITE019112		Click to Show/Hide the Full List
mod site	chr14:24103247-24103248:+		[4]
Sequence	GAGTCCACTGCTGCAGCAGAACACAAAGGTGAGCACCCTCA
Motif Score	2.951386905
Cell/Tissue List	hESC-HEK293T
Seq Type List	MAZTER-seq
Transcript ID List	ENST00000545054.6; ENST00000559250.5; ENST00000561286.5; ENST00000559171.1; ENST00000558096.5; ENST00000557969.1; ENST00000216780.8
External Link	RMBase: m6A_site_241097
mod ID: M6ASITE019113		Click to Show/Hide the Full List
mod site	chr14:24103557-24103558:+		[4]
Sequence	CATGCGGCCCTTTTTTGGCTACAACTTCGGGCACTACCTGG
Motif Score	2.078666667
Cell/Tissue List	hESC-HEK293T
Seq Type List	MAZTER-seq
Transcript ID List	ENST00000216780.8; ENST00000545054.6; ENST00000559250.5; ENST00000559171.1; ENST00000558096.5; ENST00000557969.1; ENST00000561286.5
External Link	RMBase: m6A_site_241098
mod ID: M6ASITE019114		Click to Show/Hide the Full List
mod site	chr14:24103579-24103580:+		[5]
Sequence	AACTTCGGGCACTACCTGGAACACTGGCTGAGCATGGAAGG
Motif Score	2.951386905
Cell/Tissue List	kidney; liver
Seq Type List	m6A-REF-seq
Transcript ID List	ENST00000216780.8; ENST00000559171.1; ENST00000559250.5; ENST00000561286.5; ENST00000557969.1; ENST00000545054.6; ENST00000558096.5
External Link	RMBase: m6A_site_241099
mod ID: M6ASITE019115		Click to Show/Hide the Full List
mod site	chr14:24103707-24103708:+		[7]
Sequence	GGAGAATGCTCGGGTGCTAGACTGGATCTGCCGGCGGTTAG
Motif Score	3.319380952
Cell/Tissue List	AML
Seq Type List	miCLIP
Transcript ID List	ENST00000216780.8; ENST00000559250.5; ENST00000559171.1; ENST00000557969.1; ENST00000545054.6; ENST00000561286.5; ENST00000558096.5
External Link	RMBase: m6A_site_241100
mod ID: M6ASITE019116		Click to Show/Hide the Full List
mod site	chr14:24103737-24103738:+		[4]
Sequence	CCGGCGGTTAGAGGGGGAGGACAGTGCCCGAGAGACACCCA
Motif Score	3.643047619
Cell/Tissue List	hESC-HEK293T
Seq Type List	MAZTER-seq
Transcript ID List	ENST00000216780.8; ENST00000558096.5; ENST00000545054.6; ENST00000559250.5; ENST00000561286.5; ENST00000559171.1
External Link	RMBase: m6A_site_241101
mod ID: M6ASITE019117		Click to Show/Hide the Full List
mod site	chr14:24103751-24103752:+		[5]
Sequence	GGGAGGACAGTGCCCGAGAGACACCCATTGGGCTGGTGCCA
Motif Score	2.897386905
Cell/Tissue List	HEK293; kidney; liver
Seq Type List	m6A-REF-seq
Transcript ID List	ENST00000545054.6; ENST00000216780.8; ENST00000558096.5; ENST00000559250.5; ENST00000561286.5; ENST00000559171.1
External Link	RMBase: m6A_site_241102
mod ID: M6ASITE019118		Click to Show/Hide the Full List
mod site	chr14:24103812-24103813:+		[4]
Sequence	CAGCGGCCTCAGAGCTATAGACACCACTCAGCTGTTCTCCC
Motif Score	2.897386905
Cell/Tissue List	hESC-HEK293T
Seq Type List	MAZTER-seq
Transcript ID List	ENST00000558096.5; ENST00000545054.6; ENST00000559171.1; ENST00000559250.5; ENST00000216780.8; ENST00000561286.5
External Link	RMBase: m6A_site_241103
mod ID: M6ASITE019119		Click to Show/Hide the Full List
mod site	chr14:24103842-24103843:+		[8]
Sequence	GCTGTTCTCCCTCCCCAAGGACTTCTGGGAACAGGAGGTTC
Motif Score	4.065041667
Cell/Tissue List	A549
Seq Type List	m6A-CLIP/IP
Transcript ID List	ENST00000558096.5; ENST00000545054.6; ENST00000216780.8; ENST00000561286.5; ENST00000559171.1; ENST00000559250.5
External Link	RMBase: m6A_site_241104
mod ID: M6ASITE019120		Click to Show/Hide the Full List
mod site	chr14:24103866-24103867:+		[4]
Sequence	CTGGGAACAGGAGGTTCGTGACATTCGGAGCTACCTGACAG
Motif Score	2.859755952
Cell/Tissue List	hESC-HEK293T; AML
Seq Type List	MAZTER-seq; miCLIP
Transcript ID List	ENST00000545054.6; ENST00000561286.5; ENST00000559171.1; ENST00000216780.8; ENST00000558096.5; ENST00000559250.5
External Link	RMBase: m6A_site_241105
mod ID: M6ASITE019121		Click to Show/Hide the Full List
mod site	chr14:24103883-24103884:+		[5]
Sequence	GTGACATTCGGAGCTACCTGACAGAGCAGGTCAACCAGGAT
Motif Score	2.859755952
Cell/Tissue List	HEK293
Seq Type List	m6A-REF-seq
Transcript ID List	ENST00000559171.1; ENST00000216780.8; ENST00000558096.5; ENST00000561286.5; ENST00000559250.5; ENST00000545054.6
External Link	RMBase: m6A_site_241106
mod ID: M6ASITE019122		Click to Show/Hide the Full List
mod site	chr14:24103945-24103946:+		[6]
Sequence	GAGCTTGAGGCCCTGGAGAGACGTGTGCACAAAATGTGACC
Motif Score	2.871321429
Cell/Tissue List	HEK293T; A549
Seq Type List	DART-seq; m6A-CLIP/IP
Transcript ID List	ENST00000559171.1; ENST00000216780.8; ENST00000545054.6; ENST00000558096.5; ENST00000561286.5; ENST00000559250.5
External Link	RMBase: m6A_site_241107
mod ID: M6ASITE019123		Click to Show/Hide the Full List
mod site	chr14:24103953-24103954:+		[6]
Sequence	GGCCCTGGAGAGACGTGTGCACAAAATGTGACCTGAGGCCC
Motif Score	2.830589286
Cell/Tissue List	HEK293T
Seq Type List	DART-seq
Transcript ID List	ENST00000558096.5; ENST00000559171.1; ENST00000559250.5; ENST00000561286.5; ENST00000216780.8; ENST00000545054.6
External Link	RMBase: m6A_site_241108
mod ID: M6ASITE019124		Click to Show/Hide the Full List
mod site	chr14:24103989-24103990:+		[5]
Sequence	GGCCCTAGTCTAGCAAGAGGACATAGCACCCTCATCTGGGA
Motif Score	3.643047619
Cell/Tissue List	liver; hESC-HEK293T
Seq Type List	m6A-REF-seq; MAZTER-seq
Transcript ID List	ENST00000558096.5; ENST00000559250.5; ENST00000559171.1; ENST00000561286.5; ENST00000216780.8; ENST00000545054.6
External Link	RMBase: m6A_site_241109
mod ID: M6ASITE019125		Click to Show/Hide the Full List
mod site	chr14:24104053-24104054:+		[6]
Sequence	ATATGAGCAATTTGATATTAACTAACATCTTCAATGTGCCA
Motif Score	2.590089286
Cell/Tissue List	HEK293T
Seq Type List	DART-seq
Transcript ID List	ENST00000559171.1; ENST00000216780.8; ENST00000558096.5; ENST00000559250.5; ENST00000561286.5
External Link	RMBase: m6A_site_241110
mod ID: M6ASITE019126		Click to Show/Hide the Full List
mod site	chr14:24104057-24104058:+		[4]
Sequence	GAGCAATTTGATATTAACTAACATCTTCAATGTGCCATAGA
Motif Score	2.168095238
Cell/Tissue List	hESC-HEK293T
Seq Type List	MAZTER-seq
Transcript ID List	ENST00000559250.5; ENST00000559171.1; ENST00000216780.8; ENST00000558096.5; ENST00000561286.5
External Link	RMBase: m6A_site_241111
mod ID: M6ASITE019127		Click to Show/Hide the Full List
mod site	chr14:24104077-24104078:+		[8]
Sequence	ACATCTTCAATGTGCCATAGACCTTCCCACAAAGACTGTCC
Motif Score	2.876744048
Cell/Tissue List	A549
Seq Type List	m6A-CLIP/IP
Transcript ID List	ENST00000559250.5; ENST00000559171.1; ENST00000216780.8; ENST00000558096.5; ENST00000561286.5
External Link	RMBase: m6A_site_241112
mod ID: M6ASITE019128		Click to Show/Hide the Full List
mod site	chr14:24104091-24104092:+		[7]
Sequence	CCATAGACCTTCCCACAAAGACTGTCCAATAATAAGAGATG
Motif Score	3.319380952
Cell/Tissue List	AML
Seq Type List	miCLIP
Transcript ID List	ENST00000559250.5; ENST00000558096.5; ENST00000559171.1; ENST00000561286.5; ENST00000216780.8
External Link	RMBase: m6A_site_241113

Ref 1	AZD3514: a small molecule that modulates androgen receptor signaling and function in vitro and in vivo. Mol Cancer Ther. 2013 Sep;12(9):1715-27. doi: 10.1158/1535-7163.MCT-12-1174. Epub 2013 Jul 16.
Ref 2	NAT10-mediated N4-acetylcytidine mRNA modification regulates self-renewal in human embryonic stem cells. Nucleic Acids Res. 2023 Sep 8;51(16):8514-8531. doi: 10.1093/nar/gkad628.
Ref 3	5-methylcytosine promotes mRNA export - NSUN2 as the methyltransferase and ALYREF as an m(5)C reader. Cell Res. 2017 May;27(5):606-625. doi: 10.1038/cr.2017.55. Epub 2017 Apr 18.
Ref 4	Deciphering the "m(6)A Code" via Antibody-Independent Quantitative Profiling. Cell. 2019 Jul 25;178(3):731-747.e16. doi: 10.1016/j.cell.2019.06.013. Epub 2019 Jun 27.
Ref 5	Single-base mapping of m(6)A by an antibody-independent method. Sci Adv. 2019 Jul 3;5(7):eaax0250. doi: 10.1126/sciadv.aax0250. eCollection 2019 Jul.
Ref 6	DART-seq: an antibody-free method for global m(6)A detection. Nat Methods. 2019 Dec;16(12):1275-1280. doi: 10.1038/s41592-019-0570-0. Epub 2019 Sep 23.
Ref 7	The N(6)-methyladenosine (m(6)A)-forming enzyme METTL3 controls myeloid differentiation of normal hematopoietic and leukemia cells. Nat Med. 2017 Nov;23(11):1369-1376. doi: 10.1038/nm.4416. Epub 2017 Sep 18.
Ref 8	A majority of m6A residues are in the last exons, allowing the potential for 3' UTR regulation. Genes Dev. 2015 Oct 1;29(19):2037-53. doi: 10.1101/gad.269415.115. Epub 2015 Sep 24.

m6A Target Gene Information

Cite M6AREG

Correspondence

Prof. Feng ZHU

Prof. Shuiping Liu

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