m6A Target Gene Information

General Information of the m6A Target Gene (ID: M6ATAR00663)

Target Name	Urokinase-type plasminogen activator (PLAU)
Synonyms	U-plasminogen activator; uPA Click to Show/Hide
Gene Name	PLAU
Chromosomal Location	10q22.2
Family	Peptidase S1 family
Function	Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin. Click to Show/Hide
Gene ID	5328
Uniprot ID	UROK_HUMAN
HGNC ID	HGNC:9052
KEGG ID	hsa:5328

Full List of m6A Methylation Regulator of This Target Gene and Corresponding Disease/Drug Response(s)

PLAU can be regulated by the following regulator(s), and cause disease/drug response(s). You can browse detail information of regulator(s) or disease/drug response(s).

Browse Regulator

Browse Disease

Methyltransferase-like 3 (METTL3) [WRITER]

Regulator Info

*Representative RNA-seq result indicating the expression of this target gene regulated by METTL3*
Cell Line	LX2 cell line	Homo sapiens
Treatment: shMETTL3 LX2 cells Control: shLuc LX2 cells		GSE207909
Regulation		logFC: 1.68E+00 p-value: 4.01E-99
More Results	Click to View More RNA-seq Results

In total 1 item(s) under this regulator
Experiment 1 Reporting the m6A Methylation Regulator of This Target Gene				[1]
Response Summary	METTL3 upregulated Urokinase-type plasminogen activator (PLAU) mRNA in an m6A-dependent manner, and then participated in MAPK/ERK pathway to promote angiogenesis and metastasis in CRC.
Target Regulation	Up regulation
Responsed Disease	Colorectal cancer		ICD-11: 2B91	Disease Info
Pathway Response	MAPK signaling pathway			hsa04010
In-vitro Model	LoVo	Colon adenocarcinoma	Homo sapiens	CVCL_0399
In-vitro Model	HT29	Colon cancer	Mus musculus	CVCL_A8EZ
In-vivo Model	1 × 10⁶ cells in 100 uL PBS (shMETTL3-1 or shNC) were respectively injected into each mouse through the tail vein. Pulmonary metastases were monitored after fourteen days using the imaging system (IVIS) Spectrum (PerkinElmer, USA).

Colorectal cancer [ICD-11: 2B91]

Disease Info

In total 1 item(s) under this disease
Experiment 1 Reporting the m6A-centered Disease Response				[1]
Response Summary	METTL3 upregulated Urokinase-type plasminogen activator (PLAU) mRNA in an m6A-dependent manner, and then participated in MAPK/ERK pathway to promote angiogenesis and metastasis in CRC.
Responsed Disease	Colorectal cancer [ICD-11: 2B91]
Target Regulator	Methyltransferase-like 3 (METTL3)		WRITER	Regulator Info
Target Regulation	Up regulation
Pathway Response	MAPK signaling pathway			hsa04010
In-vitro Model	LoVo	Colon adenocarcinoma	Homo sapiens	CVCL_0399
In-vitro Model	HT29	Colon cancer	Mus musculus	CVCL_A8EZ
In-vivo Model	1 × 10⁶ cells in 100 uL PBS (shMETTL3-1 or shNC) were respectively injected into each mouse through the tail vein. Pulmonary metastases were monitored after fourteen days using the imaging system (IVIS) Spectrum (PerkinElmer, USA).

Full List of Crosstalk(s) between m6A Modification and Epigenetic Regulation Related to This Regulator

Histone modification

m6A Regulator: Methyltransferase-like 3 (METTL3)

Regulator Info

In total 2 item(s) under this m6A regulator
Crosstalk ID: M6ACROT03573		Crosstalk Info
Epigenetic Regulator	Histone acetyltransferase p300 (P300)
Regulated Target	Histone H3 lysine 27 acetylation (H3K27ac)
Crosstalk relationship	Histone modification → m6A
Disease	Colorectal cancer	Disease Info
Crosstalk ID: M6ACROT03618		Crosstalk Info
Epigenetic Regulator	N-lysine methyltransferase SMYD2 (SMYD2)
Regulated Target	Histone H3 lysine 4 trimethylation (H3K4me3)
Crosstalk relationship	Histone modification → m6A
Disease	Colorectal cancer	Disease Info

RNA Modification Sequencing Data Associated with the Target (ID: M6ATAR00663)

Urokinase-type plasminogen activator (PLAU)

N6-methyladenosine (m6A)

In total 31 m6A sequence/site(s) in this target gene
mod ID: M6ASITE000844		Click to Show/Hide the Full List
mod site	chr10:73910700-73910701:+		[3]
Sequence	CATTTGTCGCGTTGATGAAGACTTCACAGCTCCATCAGCTG
Motif Score	3.319380952
Cell/Tissue List	U2OS; A549; MSC; TIME
Seq Type List	MeRIP-seq; m6A-seq
Transcript ID List	ENST00000481390.5; ENST00000496926.1
External Link	RMBase: m6A_site_106684
mod ID: M6ASITE000845		Click to Show/Hide the Full List
mod site	chr10:73911142-73911143:+		[4]
Sequence	GGTCGCAGCACAGTGCGGAGACCGCAGCCCCGGAGCCCGGG
Motif Score	2.876744048
Cell/Tissue List	A549; H1299; Jurkat; GSC-11; HEK293T; HEK293A-TOA; iSLK; MSC; TIME; HEC-1-A
Seq Type List	MeRIP-seq; m6A-seq
Transcript ID List	ENST00000446342.5; ENST00000496926.1; ENST00000372764.4; ENST00000481390.5
External Link	RMBase: m6A_site_106688
mod ID: M6ASITE000846		Click to Show/Hide the Full List
mod site	chr10:73911649-73911650:+		[4]
Sequence	TTGACTGATGCTGCCCAAGGACCTCTGATCAGCACCAGGGG
Motif Score	3.622404762
Cell/Tissue List	A549; Jurkat; peripheral-blood; iSLK; MSC; TIME; endometrial; HEC-1-A; GSCs; NB4; MM6
Seq Type List	MeRIP-seq; m6A-seq
Transcript ID List	ENST00000446342.5; ENST00000481390.5; ENST00000494287.1; ENST00000372764.4
External Link	RMBase: m6A_site_106693
mod ID: M6ASITE000847		Click to Show/Hide the Full List
mod site	chr10:73911728-73911729:+		[5]
Sequence	TCCATCCACAGCAGGGCCAGACTCTCCCCAGGAAATGGGAC
Motif Score	3.319380952
Cell/Tissue List	H1B; Jurkat; peripheral-blood; endometrial; HEC-1-A; MM6
Seq Type List	m6A-seq
Transcript ID List	ENST00000494287.1; ENST00000372764.4; ENST00000446342.5; ENST00000481390.5
External Link	RMBase: m6A_site_106695
mod ID: M6ASITE000848		Click to Show/Hide the Full List
mod site	chr10:73911747-73911748:+		[5]
Sequence	GACTCTCCCCAGGAAATGGGACAGGGTGGCAGCGGAGGCTT
Motif Score	3.643047619
Cell/Tissue List	H1B; Jurkat; peripheral-blood; endometrial; HEC-1-A
Seq Type List	m6A-seq
Transcript ID List	ENST00000494287.1; ENST00000446342.5; ENST00000372764.4; ENST00000481390.5
External Link	RMBase: m6A_site_106696
mod ID: M6ASITE000849		Click to Show/Hide the Full List
mod site	chr10:73911772-73911773:+		[5]
Sequence	GTGGCAGCGGAGGCTTGAGAACCACGGGGGTTGGCACTGGC
Motif Score	2.930744048
Cell/Tissue List	H1B; Jurkat; peripheral-blood; endometrial; HEC-1-A
Seq Type List	m6A-seq
Transcript ID List	ENST00000372764.4; ENST00000494287.1; ENST00000481390.5; ENST00000446342.5
External Link	RMBase: m6A_site_106697
mod ID: M6ASITE000850		Click to Show/Hide the Full List
mod site	chr10:73911819-73911820:+		[6]
Sequence	GGGAGGAAGAGGCCGCCGGGACTGCCCCAGCCTGCGGGCAT
Motif Score	4.065041667
Cell/Tissue List	Jurkat; peripheral-blood; endometrial; HEC-1-A
Seq Type List	m6A-seq
Transcript ID List	ENST00000481390.5; ENST00000494287.1; ENST00000372764.4; ENST00000446342.5
External Link	RMBase: m6A_site_106698
mod ID: M6ASITE000851		Click to Show/Hide the Full List
mod site	chr10:73911887-73911888:+		[7]
Sequence	TCCATTTCTCCTGGCTGGAAACCCATGGTCTTCCATTTGAG
Motif Score	2.185083333
Cell/Tissue List	peripheral-blood; HEC-1-A
Seq Type List	m6A-seq
Transcript ID List	ENST00000372764.4; ENST00000494287.1; ENST00000481390.5; ENST00000446342.5
External Link	RMBase: m6A_site_106699
mod ID: M6ASITE000852		Click to Show/Hide the Full List
mod site	chr10:73911909-73911910:+		[7]
Sequence	CCATGGTCTTCCATTTGAGAACTAGATACGAACAGGGTGAG
Motif Score	3.373380952
Cell/Tissue List	peripheral-blood; HEC-1-A
Seq Type List	m6A-seq
Transcript ID List	ENST00000446342.5; ENST00000372764.4; ENST00000494287.1; ENST00000481390.5
External Link	RMBase: m6A_site_106701
mod ID: M6ASITE000853		Click to Show/Hide the Full List
mod site	chr10:73911920-73911921:+		[7]
Sequence	CATTTGAGAACTAGATACGAACAGGGTGAGGCGAGAGGGAG
Motif Score	2.951386905
Cell/Tissue List	peripheral-blood; HEC-1-A
Seq Type List	m6A-seq
Transcript ID List	ENST00000494287.1; ENST00000446342.5; ENST00000481390.5; ENST00000372764.4
External Link	RMBase: m6A_site_106702
mod ID: M6ASITE000854		Click to Show/Hide the Full List
mod site	chr10:73912051-73912052:+		[7]
Sequence	CTCTTCCAGGGCAGCAATGAACTTCATCAAGTTCCATGTGA
Motif Score	3.373380952
Cell/Tissue List	peripheral-blood; HEC-1-A
Seq Type List	m6A-seq
Transcript ID List	ENST00000372764.4; ENST00000446342.5; ENST00000481390.5; ENST00000494287.1
External Link	RMBase: m6A_site_106703
mod ID: M6ASITE000855		Click to Show/Hide the Full List
mod site	chr10:73912217-73912218:+		[8]
Sequence	ACCTTTGTTCTCTCCAGCGAACTGTGACTGTCTAAATGGAG
Motif Score	3.373380952
Cell/Tissue List	HEC-1-A
Seq Type List	m6A-seq
Transcript ID List	ENST00000372764.4; ENST00000494287.1; ENST00000446342.5; ENST00000481390.5
External Link	RMBase: m6A_site_106704
mod ID: M6ASITE000856		Click to Show/Hide the Full List
mod site	chr10:73912240-73912241:+		[8]
Sequence	GTGACTGTCTAAATGGAGGAACATGTGTGTCCAACAAGTAC
Motif Score	2.951386905
Cell/Tissue List	HEC-1-A
Seq Type List	m6A-seq
Transcript ID List	ENST00000481390.5; ENST00000494287.1; ENST00000446342.5; ENST00000372764.4
External Link	RMBase: m6A_site_106705
mod ID: M6ASITE000857		Click to Show/Hide the Full List
mod site	chr10:73912983-73912984:+		[9]
Sequence	CCGAGGAAAGGCCAGCACTGACACCATGGGCCGGCCCTGCC
Motif Score	2.859755952
Cell/Tissue List	kidney
Seq Type List	m6A-REF-seq
Transcript ID List	ENST00000481390.5; ENST00000446342.5; ENST00000372764.4; ENST00000494287.1
External Link	RMBase: m6A_site_106706
mod ID: M6ASITE000858		Click to Show/Hide the Full List
mod site	chr10:73914002-73914003:+		[9]
Sequence	TTACCCAAAGAAGGAGGACTACATCGTCTACCTGGGTCGCT
Motif Score	2.078666667
Cell/Tissue List	kidney
Seq Type List	m6A-REF-seq
Transcript ID List	ENST00000372764.4; ENST00000446342.5
External Link	RMBase: m6A_site_106707
mod ID: M6ASITE000859		Click to Show/Hide the Full List
mod site	chr10:73914071-73914072:+		[10]
Sequence	GATGAAGTTTGAGGTGGAAAACCTCATCCTACACAAGGACT
Motif Score	2.185083333
Cell/Tissue List	MT4; HEC-1-A
Seq Type List	MeRIP-seq; m6A-seq
Transcript ID List	ENST00000446342.5; ENST00000372764.4
External Link	RMBase: m6A_site_106708
mod ID: M6ASITE000860		Click to Show/Hide the Full List
mod site	chr10:73914089-73914090:+		[10]
Sequence	AAACCTCATCCTACACAAGGACTACAGCGCTGACACGCTTG
Motif Score	4.065041667
Cell/Tissue List	MT4; HEC-1-A
Seq Type List	MeRIP-seq; m6A-seq
Transcript ID List	ENST00000372764.4; ENST00000446342.5
External Link	RMBase: m6A_site_106709
mod ID: M6ASITE000861		Click to Show/Hide the Full List
mod site	chr10:73914101-73914102:+		[9]
Sequence	ACACAAGGACTACAGCGCTGACACGCTTGCTCACCACAACG
Motif Score	2.859755952
Cell/Tissue List	kidney
Seq Type List	m6A-REF-seq
Transcript ID List	ENST00000446342.5; ENST00000372764.4
External Link	RMBase: m6A_site_106710
mod ID: M6ASITE000862		Click to Show/Hide the Full List
mod site	chr10:73914825-73914826:+		[8]
Sequence	GGTGTGCGCAGCCATCCCGGACTATACAGACCATCTGCCTG
Motif Score	4.065041667
Cell/Tissue List	HEC-1-A
Seq Type List	m6A-seq
Transcript ID List	ENST00000372764.4; ENST00000446342.5
External Link	RMBase: m6A_site_106711
mod ID: M6ASITE000863		Click to Show/Hide the Full List
mod site	chr10:73914834-73914835:+		[8]
Sequence	AGCCATCCCGGACTATACAGACCATCTGCCTGCCCTCGATG
Motif Score	2.876744048
Cell/Tissue List	HEC-1-A
Seq Type List	m6A-seq
Transcript ID List	ENST00000446342.5; ENST00000372764.4
External Link	RMBase: m6A_site_106712
mod ID: M6ASITE000864		Click to Show/Hide the Full List
mod site	chr10:73914876-73914877:+		[9]
Sequence	ATAACGATCCCCAGTTTGGCACAAGCTGTGAGATCACTGGC
Motif Score	2.830589286
Cell/Tissue List	kidney
Seq Type List	m6A-REF-seq
Transcript ID List	ENST00000372764.4; ENST00000446342.5
External Link	RMBase: m6A_site_106713
mod ID: M6ASITE000865		Click to Show/Hide the Full List
mod site	chr10:73915384-73915385:+		[8]
Sequence	CTGCTGACCCACAGTGGAAAACAGATTCCTGCCAGGTGAGT
Motif Score	2.20572619
Cell/Tissue List	HEC-1-A
Seq Type List	m6A-seq
Transcript ID List	ENST00000372764.4; ENST00000446342.5
External Link	RMBase: m6A_site_106714
mod ID: M6ASITE000866		Click to Show/Hide the Full List
mod site	chr10:73916392-73916393:+		[8]
Sequence	ATCTTTGGCGTCACAGGGAGACTCAGGGGGACCCCTCGTCT
Motif Score	3.319380952
Cell/Tissue List	HEC-1-A
Seq Type List	m6A-seq
Transcript ID List	ENST00000372764.4; ENST00000446342.5
External Link	RMBase: m6A_site_106715
mod ID: M6ASITE000867		Click to Show/Hide the Full List
mod site	chr10:73916402-73916403:+		[11]
Sequence	TCACAGGGAGACTCAGGGGGACCCCTCGTCTGTTCCCTCCA
Motif Score	3.622404762
Cell/Tissue List	MM6; Jurkat; HEC-1-A
Seq Type List	m6A-seq
Transcript ID List	ENST00000372764.4; ENST00000446342.5
External Link	RMBase: m6A_site_106716
mod ID: M6ASITE000868		Click to Show/Hide the Full List
mod site	chr10:73916479-73916480:+		[12]
Sequence	CCGTGGATGTGCCCTGAAGGACAAGCCAGGCGTCTACACGA
Motif Score	3.643047619
Cell/Tissue List	hESC-HEK293T; U2OS; A549; MM6; Jurkat; peripheral-blood; endometrial; HEC-1-A; AML
Seq Type List	MAZTER-seq; MeRIP-seq; m6A-seq; miCLIP
Transcript ID List	ENST00000446342.5; ENST00000372764.4
External Link	RMBase: m6A_site_106717
mod ID: M6ASITE000869		Click to Show/Hide the Full List
mod site	chr10:73916653-73916654:+		[13]
Sequence	TCCATCAGCTGTAAGAAGAGACTGGGAAGATAGGCTCTGCA
Motif Score	3.319380952
Cell/Tissue List	HeLa; HepG2; HEK293T; A549; U2OS; H1A; H1B; hNPCs; hESCs; fibroblasts; GM12878; H1299; MM6; Jurkat; peripheral-blood; GSC-11; HEK293A-TOA; iSLK; MSC; TIME; endometrial; HEC-1-A; NB4
Seq Type List	m6A-seq; MeRIP-seq; m6A-CLIP/IP
Transcript ID List	ENST00000446342.5; ENST00000372764.4
External Link	RMBase: m6A_site_106719
mod ID: M6ASITE000870		Click to Show/Hide the Full List
mod site	chr10:73916711-73916712:+		[12]
Sequence	GCCACCCACCAGGGCGAACGACAATAGCTTTACCCTCAGGC
Motif Score	2.865571429
Cell/Tissue List	hESC-HEK293T; A549
Seq Type List	MAZTER-seq; m6A-CLIP/IP
Transcript ID List	ENST00000372764.4; ENST00000446342.5
External Link	RMBase: m6A_site_106720
mod ID: M6ASITE000871		Click to Show/Hide the Full List
mod site	chr10:73916757-73916758:+		[13]
Sequence	CCTGGGTGCTGGCTGCCCAGACCCCTCTGGCCAGGATGGAG
Motif Score	2.876744048
Cell/Tissue List	HeLa; HepG2; HEK293T; A549; U2OS; H1A; H1B; hNPCs; hESCs; fibroblasts; GM12878; H1299; MM6; Jurkat; CD4T; peripheral-blood; GSC-11; HEK293A-TOA; iSLK; MSC; TIME; endometrial; HEC-1-A; NB4
Seq Type List	m6A-seq; MeRIP-seq
Transcript ID List	ENST00000446342.5; ENST00000372764.4
External Link	RMBase: m6A_site_106721
mod ID: M6ASITE000872		Click to Show/Hide the Full List
mod site	chr10:73916828-73916829:+		[14]
Sequence	AGCAACTTGTCTTTTTCTGGACTGAAGCCTGCAGGAGTTAA
Motif Score	4.065041667
Cell/Tissue List	HepG2; A549; U2OS; H1A; H1B; fibroblasts; GM12878; H1299; MM6; CD4T; peripheral-blood; GSC-11; HEK293A-TOA; iSLK; MSC; TIME; endometrial; HEC-1-A; NB4; AML
Seq Type List	m6A-seq; MeRIP-seq; m6A-CLIP/IP; miCLIP
Transcript ID List	ENST00000372764.4; ENST00000446342.5
External Link	RMBase: m6A_site_106722
mod ID: M6ASITE000873		Click to Show/Hide the Full List
mod site	chr10:73917021-73917022:+		[15]
Sequence	CAGCGGTTTGGGGAGCAGAGACACTAACGACTTCAGGGCAG
Motif Score	2.897386905
Cell/Tissue List	A549; H1299; HEC-1-A
Seq Type List	m6A-seq
Transcript ID List	ENST00000372764.4; ENST00000446342.5
External Link	RMBase: m6A_site_106723
mod ID: M6ASITE000874		Click to Show/Hide the Full List
mod site	chr10:73917256-73917257:+		[9]
Sequence	CCTCTTGGGTCCCCCACGTGACAGTGCCTGGGAATGTATTA
Motif Score	2.859755952
Cell/Tissue List	kidney
Seq Type List	m6A-REF-seq
Transcript ID List	ENST00000446342.5; ENST00000372764.4
External Link	RMBase: m6A_site_106724

Ref 1	METTL3 promotes colorectal cancer metastasis by stabilizing PLAU mRNA in an m6A-dependent manner. Biochem Biophys Res Commun. 2022 Jul 23;614:9-16. doi: 10.1016/j.bbrc.2022.04.141. Epub 2022 May 6.
Ref 2	Indomethacin induces apoptosis via a MRP1-dependent mechanism in doxorubicin-resistant small-cell lung cancer cells overexpressing MRP1. Br J Cancer. 2007 Oct 22;97(8):1077-83. doi: 10.1038/sj.bjc.6604010. Epub 2007 Oct 16.
Ref 3	RNA-methylation-dependent RNA processing controls the speed of the circadian clock. Cell. 2013 Nov 7;155(4):793-806. doi: 10.1016/j.cell.2013.10.026.
Ref 4	Viral N(6)-methyladenosine upregulates replication and pathogenesis of human respiratory syncytial virus. Nat Commun. 2019 Oct 9;10(1):4595. doi: 10.1038/s41467-019-12504-y.
Ref 5	m(6)A RNA modification controls cell fate transition in mammalian embryonic stem cells. Cell Stem Cell. 2014 Dec 4;15(6):707-19. doi: 10.1016/j.stem.2014.09.019. Epub 2014 Oct 16.
Ref 6	N(6)-methyladenosine of HIV-1 RNA regulates viral infection and HIV-1 Gag protein expression. Elife. 2016 Jul 2;5:e15528. doi: 10.7554/eLife.15528.
Ref 7	R-2HG Exhibits Anti-tumor Activity by Targeting FTO/m(6)A/MYC/CEBPA Signaling. Cell. 2018 Jan 11;172(1-2):90-105.e23. doi: 10.1016/j.cell.2017.11.031. Epub 2017 Dec 14.
Ref 8	m(6)A mRNA methylation regulates AKT activity to promote the proliferation and tumorigenicity of endometrial cancer. Nat Cell Biol. 2018 Sep;20(9):1074-1083. doi: 10.1038/s41556-018-0174-4. Epub 2018 Aug 27.
Ref 9	Single-base mapping of m(6)A by an antibody-independent method. Sci Adv. 2019 Jul 3;5(7):eaax0250. doi: 10.1126/sciadv.aax0250. eCollection 2019 Jul.
Ref 10	Dynamics of the human and viral m(6)A RNA methylomes during HIV-1 infection of T cells. Nat Microbiol. 2016 Feb 22;1:16011. doi: 10.1038/nmicrobiol.2016.11.
Ref 11	FTO Plays an Oncogenic Role in Acute Myeloid Leukemia as a N(6)-Methyladenosine RNA Demethylase. Cancer Cell. 2017 Jan 9;31(1):127-141. doi: 10.1016/j.ccell.2016.11.017. Epub 2016 Dec 22.
Ref 12	Deciphering the "m(6)A Code" via Antibody-Independent Quantitative Profiling. Cell. 2019 Jul 25;178(3):731-747.e16. doi: 10.1016/j.cell.2019.06.013. Epub 2019 Jun 27.
Ref 13	VIRMA mediates preferential m(6)A mRNA methylation in 3'UTR and near stop codon and associates with alternative polyadenylation. Cell Discov. 2018 Feb 27;4:10. doi: 10.1038/s41421-018-0019-0. eCollection 2018.
Ref 14	Histone H3 trimethylation at lysine 36 guides m(6)A RNA modification co-transcriptionally. Nature. 2019 Mar;567(7748):414-419. doi: 10.1038/s41586-019-1016-7. Epub 2019 Mar 13.
Ref 15	Perturbation of m6A writers reveals two distinct classes of mRNA methylation at internal and 5' sites. Cell Rep. 2014 Jul 10;8(1):284-96. doi: 10.1016/j.celrep.2014.05.048. Epub 2014 Jun 26.

m6A Target Gene Information

Cite M6AREG

Correspondence

Prof. Feng ZHU

Prof. Shuiping Liu

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