m6A Target Gene Information

General Information of the m6A Target Gene (ID: M6ATAR00553)
Target Name Zinc finger and BTB domain-containing protein 4 (ZBTB4)
Synonyms
KAISO-like zinc finger protein 1; KAISO-L1
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Gene Name ZBTB4
Chromosomal Location 17p13.1
Function
Transcriptional repressor with bimodal DNA-binding specificity. Represses transcription in a methyl-CpG-dependent manner. Binds with a higher affinity to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' but can also bind to the non-methylated consensus sequence 5'-CTGCNA-3' also known as the consensus kaiso binding site (KBS). Can also bind specifically to a single methyl-CpG pair and can bind hemimethylated DNA but with a lower affinity compared to methylated DNA . Plays a role in postnatal myogenesis, may be involved in the regulation of satellite cells self-renewal (By similarity).
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Gene ID 57659
Uniprot ID
ZBTB4_HUMAN
HGNC ID
HGNC:23847
Ensembl Gene ID
ENSG00000174282; ENSG00000283868
KEGG ID
hsa:57659
Full List of m6A Methylation Regulator of This Target Gene and Corresponding Disease/Drug Response(s)
ZBTB4 can be regulated by the following regulator(s), and cause disease/drug response(s). You can browse detail information of regulator(s) or disease/drug response(s).
Browse Regulator
Browse Disease
Methyltransferase-like 3 (METTL3) [WRITER]
 Regulator Info 
Representative RNA-seq result indicating the expression of this target gene regulated by METTL3
Cell Line LX2 cell line Homo sapiens
Treatment: shMETTL3 LX2 cells
Control: shLuc LX2 cells
 GSE207909
Regulation
logFC: 8.19E-01
p-value: 3.59E-20
More Results Click to View More RNA-seq Results
Representative RIP-seq result supporting the interaction between ZBTB4 and the regulator
Cell Line MDA-MB-231 Homo sapiens
Regulation logFC: 1.32E+00 GSE60213
In total 1 item(s) under this regulator
Experiment 1 Reporting the m6A Methylation Regulator of This Target Gene [1]
Response Summary METTL3-mediated m6A modification of Zinc finger and BTB domain-containing protein 4 (ZBTB4) via EZH2 is involved in the CS-induced EMT and in lung cancer.
Target Regulation Down regulation
Responsed Disease Lung cancer ICD-11: 2C25
 Disease Info 
Pathway Response RNA degradation hsa03018
Cell Process Epithelial-mesenchymal transition
In-vitro Model HBE (Human bronchial epithelial cell line)
In-vivo Model Male BALB/c mice were grouped into control, CS, CS+AAV-ShMETTL3, and CS+AAV-NC shRNA groups, n = 6 animals per group. Mice in the AAV-ShMETTL3 and AAV-NC shRNA (GeneChem, China) groups were dosed by intranasal instillation after CS exposure for 4 weeks.
Lung cancer [ICD-11: 2C25]
 Disease Info 
In total 1 item(s) under this disease
Experiment 1 Reporting the m6A-centered Disease Response [1]
Response Summary METTL3-mediated m6A modification of Zinc finger and BTB domain-containing protein 4 (ZBTB4) via EZH2 is involved in the CS-induced EMT and in lung cancer.
Responsed Disease Lung cancer [ICD-11: 2C25]
Target Regulator Methyltransferase-like 3 (METTL3) WRITER
 Regulator Info 
Target Regulation Down regulation
Pathway Response RNA degradation hsa03018
Cell Process Epithelial-mesenchymal transition
In-vitro Model HBE (Human bronchial epithelial cell line)
In-vivo Model Male BALB/c mice were grouped into control, CS, CS+AAV-ShMETTL3, and CS+AAV-NC shRNA groups, n = 6 animals per group. Mice in the AAV-ShMETTL3 and AAV-NC shRNA (GeneChem, China) groups were dosed by intranasal instillation after CS exposure for 4 weeks.
References
Ref 1 METTL3-mediated m(6)A modification of ZBTB4 mRNA is involved in the smoking-induced EMT in cancer of the lung. Mol Ther Nucleic Acids. 2020 Dec 10;23:487-500. doi: 10.1016/j.omtn.2020.12.001. eCollection 2021 Mar 5.