m6A Target Gene Information
General Information of the m6A Target Gene (ID: M6ATAR00484)
Full List of m6A Methylation Regulator of This Target Gene and Corresponding Disease/Drug Response(s)
SLC7A11
can be regulated by the following regulator(s), and cause disease/drug response(s). You can browse detail information of regulator(s) or disease/drug response(s).
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Insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1) [READER]
Representative RNA-seq result indicating the expression of this target gene regulated by IGF2BP1 | ||
Cell Line | MV3 cell line | Homo sapiens |
Treatment: siIGF2BP1 MV3 cells
Control: siControl MV3 cells
|
GSE146803 | |
Regulation |
|
logFC: -1.30E+00 p-value: 3.62E-06 |
More Results | Click to View More RNA-seq Results |
In total 1 item(s) under this regulator | ||||
Experiment 1 Reporting the m6A Methylation Regulator of This Target Gene | [1] | |||
Response Summary | METTL3-mediated Cystine/glutamate transporter (SLC7A11) m6A modification enhances hepatoblastoma ferroptosis resistance. The METTL3/IGF2BP1/m6A modification promotes SLC7A11 mRNA stability and upregulates its expression by inhibiting the deadenylation process. | |||
Target Regulation | Up regulation | |||
Responsed Disease | Hepatoblastoma | ICD-11: 2C12.01 | ||
Pathway Response | Ferroptosis | hsa04216 | ||
Cell Process | Ferroptosis | |||
In-vitro Model | HuH-6 | Hepatoblastoma | Homo sapiens | CVCL_4381 |
Hep-G2 | Hepatoblastoma | Homo sapiens | CVCL_0027 | |
HEK293T | Normal | Homo sapiens | CVCL_0063 | |
Methyltransferase-like 14 (METTL14) [WRITER]
Representative RNA-seq result indicating the expression of this target gene regulated by METTL14 | ||
Cell Line | HepG2 cell line | Homo sapiens |
Treatment: shMETTL14 HepG2 cells
Control: shCtrl HepG2 cells
|
GSE121949 | |
Regulation |
|
logFC: -1.11E+00 p-value: 3.96E-11 |
More Results | Click to View More RNA-seq Results |
In total 4 item(s) under this regulator | ||||
Experiment 1 Reporting the m6A Methylation Regulator of This Target Gene | [2] | |||
Response Summary | METTL14 induced m6A modification at 5'UTR of Cystine/glutamate transporter (SLC7A11) mRNA, which in turn underwent degradation relied on the YTHDF2-dependent pathway. Identify the HIF-1alpha /METTL14/YTHDF2/SLC7A11 axis as a potential therapeutic target for the HCC interventional embolization treatment. | |||
Target Regulation | Down regulation | |||
Responsed Disease | Liver cancer | ICD-11: 2C12 | ||
Pathway Response | HIF-1 signaling pathway | hsa04066 | ||
Cell Process | RNA stability | |||
In-vitro Model | PLC/PRF/5 | Adult hepatocellular carcinoma | Homo sapiens | CVCL_0485 |
MHCC97-H | Adult hepatocellular carcinoma | Homo sapiens | CVCL_4972 | |
L-02 | Endocervical adenocarcinoma | Homo sapiens | CVCL_6926 | |
Huh-7 | Adult hepatocellular carcinoma | Homo sapiens | CVCL_0336 | |
Hep-G2 | Hepatoblastoma | Homo sapiens | CVCL_0027 | |
HCCLM3 | Adult hepatocellular carcinoma | Homo sapiens | CVCL_6832 | |
BEL-7402 | Endocervical adenocarcinoma | Homo sapiens | CVCL_5492 | |
7721 (Human hepatic malignant cell line) | ||||
In-vivo Model | For the subcutaneous implantation model, 5 × 105 stable SLC7A11-knockdown HCCLM3 cells or SLC7A11-vector cells were injected subcutaneously into BALB/C nude mice. | |||
Experiment 2 Reporting the m6A Methylation Regulator of This Target Gene | [3] | |||
Response Summary | m6A-hypomethylation regulated FGFR4 phosphorylates GSK-3beta and activates beta-catenin/TCF4-Cystine/glutamate transporter (SLC7A11)/FPN1 signaling to drive anti-HER2 resistance. Knockdown of METTL14 significantly increased the expression level of FGFR4 in HER2-positive breast cancer cells. FGFR4 reduced the sensitivity of HER2-positive breast cancer to trastuzumab plus pertuzumab or tucatinib. These results pinpoint a mechanism of anti-HER2 resistance and provide a strategy for overcoming resistance via FGFR4 inhibition in recalcitrant HER2-positive breast cancer. | |||
Target Regulation | Down regulation | |||
Responsed Disease | Breast cancer | ICD-11: 2C60 | ||
Responsed Drug | Pertuzumab | Approved | ||
Pathway Response | Wnt signaling pathway | hsa04310 | ||
Cell Process | Glutathione synthesis | |||
In-vitro Model | ZR-75-1 | Invasive breast carcinoma | Homo sapiens | CVCL_0588 |
T-47D | Invasive breast carcinoma | Homo sapiens | CVCL_0553 | |
SUM-159 (A mesenchymal triple-negative breast cancer cell line) | ||||
SK-BR-3 | Breast adenocarcinoma | Homo sapiens | CVCL_0033 | |
MDA-MB-468 | Breast adenocarcinoma | Homo sapiens | CVCL_0419 | |
MDA-MB-453 | Breast adenocarcinoma | Homo sapiens | CVCL_0418 | |
MDA-MB-361 | Breast adenocarcinoma | Homo sapiens | CVCL_0620 | |
MDA-MB-231 | Breast adenocarcinoma | Homo sapiens | CVCL_0062 | |
MCF-7 | Invasive breast carcinoma | Homo sapiens | CVCL_0031 | |
MCF-10A | Normal | Homo sapiens | CVCL_0598 | |
HEK293T | Normal | Homo sapiens | CVCL_0063 | |
BT-549 | Invasive breast carcinoma | Homo sapiens | CVCL_1092 | |
BT-474 | Invasive breast carcinoma | Homo sapiens | CVCL_0179 | |
AU565 | Breast adenocarcinoma | Homo sapiens | CVCL_1074 | |
In-vivo Model | Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs. | |||
Experiment 3 Reporting the m6A Methylation Regulator of This Target Gene | [3] | |||
Response Summary | m6A-hypomethylation regulated FGFR4 phosphorylates GSK-3beta and activates beta-catenin/TCF4-Cystine/glutamate transporter (SLC7A11)/FPN1 signaling to drive anti-HER2 resistance. Knockdown of METTL14 significantly increased the expression level of FGFR4 in HER2-positive breast cancer cells. FGFR4 reduced the sensitivity of HER2-positive breast cancer to trastuzumab plus pertuzumab or tucatinib. These results pinpoint a mechanism of anti-HER2 resistance and provide a strategy for overcoming resistance via FGFR4 inhibition in recalcitrant HER2-positive breast cancer. | |||
Target Regulation | Down regulation | |||
Responsed Disease | Breast cancer | ICD-11: 2C60 | ||
Responsed Drug | Trastuzumab | Approved | ||
Pathway Response | Wnt signaling pathway | hsa04310 | ||
Cell Process | Glutathione synthesis | |||
In-vitro Model | ZR-75-1 | Invasive breast carcinoma | Homo sapiens | CVCL_0588 |
T-47D | Invasive breast carcinoma | Homo sapiens | CVCL_0553 | |
SUM-159 (A mesenchymal triple-negative breast cancer cell line) | ||||
SK-BR-3 | Breast adenocarcinoma | Homo sapiens | CVCL_0033 | |
MDA-MB-468 | Breast adenocarcinoma | Homo sapiens | CVCL_0419 | |
MDA-MB-453 | Breast adenocarcinoma | Homo sapiens | CVCL_0418 | |
MDA-MB-361 | Breast adenocarcinoma | Homo sapiens | CVCL_0620 | |
MDA-MB-231 | Breast adenocarcinoma | Homo sapiens | CVCL_0062 | |
MCF-7 | Invasive breast carcinoma | Homo sapiens | CVCL_0031 | |
MCF-10A | Normal | Homo sapiens | CVCL_0598 | |
HEK293T | Normal | Homo sapiens | CVCL_0063 | |
BT-549 | Invasive breast carcinoma | Homo sapiens | CVCL_1092 | |
BT-474 | Invasive breast carcinoma | Homo sapiens | CVCL_0179 | |
AU565 | Breast adenocarcinoma | Homo sapiens | CVCL_1074 | |
In-vivo Model | Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs. | |||
Experiment 4 Reporting the m6A Methylation Regulator of This Target Gene | [3] | |||
Response Summary | m6A-hypomethylation regulated FGFR4 phosphorylates GSK-3beta and activates beta-catenin/TCF4-Cystine/glutamate transporter (SLC7A11)/FPN1 signaling to drive anti-HER2 resistance. Knockdown of METTL14 significantly increased the expression level of FGFR4 in HER2-positive breast cancer cells. FGFR4 reduced the sensitivity of HER2-positive breast cancer to trastuzumab plus pertuzumab or tucatinib. These results pinpoint a mechanism of anti-HER2 resistance and provide a strategy for overcoming resistance via FGFR4 inhibition in recalcitrant HER2-positive breast cancer. | |||
Target Regulation | Down regulation | |||
Responsed Disease | Breast cancer | ICD-11: 2C60 | ||
Responsed Drug | Tucatinib | Approved | ||
Pathway Response | Wnt signaling pathway | hsa04310 | ||
Cell Process | Glutathione synthesis | |||
In-vitro Model | ZR-75-1 | Invasive breast carcinoma | Homo sapiens | CVCL_0588 |
T-47D | Invasive breast carcinoma | Homo sapiens | CVCL_0553 | |
SUM-159 (A mesenchymal triple-negative breast cancer cell line) | ||||
SK-BR-3 | Breast adenocarcinoma | Homo sapiens | CVCL_0033 | |
MDA-MB-468 | Breast adenocarcinoma | Homo sapiens | CVCL_0419 | |
MDA-MB-453 | Breast adenocarcinoma | Homo sapiens | CVCL_0418 | |
MDA-MB-361 | Breast adenocarcinoma | Homo sapiens | CVCL_0620 | |
MDA-MB-231 | Breast adenocarcinoma | Homo sapiens | CVCL_0062 | |
MCF-7 | Invasive breast carcinoma | Homo sapiens | CVCL_0031 | |
MCF-10A | Normal | Homo sapiens | CVCL_0598 | |
HEK293T | Normal | Homo sapiens | CVCL_0063 | |
BT-549 | Invasive breast carcinoma | Homo sapiens | CVCL_1092 | |
BT-474 | Invasive breast carcinoma | Homo sapiens | CVCL_0179 | |
AU565 | Breast adenocarcinoma | Homo sapiens | CVCL_1074 | |
In-vivo Model | Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs. | |||
Methyltransferase-like 3 (METTL3) [WRITER]
Representative RNA-seq result indicating the expression of this target gene regulated by METTL3 | ||
Cell Line | MOLM-13 cell line | Homo sapiens |
Treatment: shMETTL3 MOLM13 cells
Control: MOLM13 cells
|
GSE98623 | |
Regulation |
|
logFC: -4.02E+00 p-value: 2.26E-108 |
More Results | Click to View More RNA-seq Results |
In total 2 item(s) under this regulator | ||||
Experiment 1 Reporting the m6A Methylation Regulator of This Target Gene | [1] | |||
Response Summary | METTL3-mediated Cystine/glutamate transporter (SLC7A11) m6A modification enhances hepatoblastoma ferroptosis resistance. The METTL3/IGF2BP1/m6A modification promotes SLC7A11 mRNA stability and upregulates its expression by inhibiting the deadenylation process. | |||
Target Regulation | Up regulation | |||
Responsed Disease | Hepatoblastoma | ICD-11: 2C12.01 | ||
Pathway Response | Ferroptosis | hsa04216 | ||
Cell Process | Ferroptosis | |||
In-vitro Model | HuH-6 | Hepatoblastoma | Homo sapiens | CVCL_4381 |
Hep-G2 | Hepatoblastoma | Homo sapiens | CVCL_0027 | |
HEK293T | Normal | Homo sapiens | CVCL_0063 | |
Experiment 2 Reporting the m6A Methylation Regulator of This Target Gene | [4] | |||
Response Summary | METTL3-mediated m-6A modification could stabilize Cystine/glutamate transporter (SLC7A11) mRNA and promote its translation, thus promoting LUAD cell proliferation and inhibiting cell ferroptosis, a novel form of programmed cell death. | |||
Target Regulation | Up regulation | |||
Responsed Disease | Lung adenocarcinoma | ICD-11: 2C25.0 | ||
Pathway Response | Ferroptosis | hsa04216 | ||
Cell Process | Ferroptosis | |||
In-vitro Model | SPC-A1 | Endocervical adenocarcinoma | Homo sapiens | CVCL_6955 |
PC-9 | Lung adenocarcinoma | Homo sapiens | CVCL_B260 | |
NCI-H460 | Lung large cell carcinoma | Homo sapiens | CVCL_0459 | |
NCI-H322 | Minimally invasive lung adenocarcinoma | Homo sapiens | CVCL_1556 | |
NCI-H1975 | Lung adenocarcinoma | Homo sapiens | CVCL_1511 | |
NCI-H1299 | Lung large cell carcinoma | Homo sapiens | CVCL_0060 | |
BEAS-2B | Normal | Homo sapiens | CVCL_0168 | |
A-549 | Lung adenocarcinoma | Homo sapiens | CVCL_0023 | |
In-vivo Model | For the subcutaneous xenograft model, PC9 cells stably transfected with METTL3 knockdown (shMETTL3) or negative control (shNC) shRNA (5 × 106 cells per mouse, n = 6) were suspended in 200 uL PBS with 50% Matrigel matrix (Corning, USA, 354234) and then injected into one side of the axilla of nude mice. | |||
NF-kappa-B-activating protein (NKAP) [READER]
In total 1 item(s) under this regulator | ||||
Experiment 1 Reporting the m6A Methylation Regulator of This Target Gene | [5] | |||
Response Summary | This study NKAP knockdown induced cell death in glioblastoma cells. NKAP acted as a new ferroptosis suppressor by binding to m6A and then promoting Cystine/glutamate transporter (SLC7A11) mRNA splicing and maturation. | |||
Target Regulation | Up regulation | |||
Responsed Disease | Glioblastoma | ICD-11: 2A00.00 | ||
Pathway Response | Ferroptosis | hsa04216 | ||
Cell Process | Ferroptosis | |||
In-vitro Model | U87MG (Astroblastoma cells from human brain) | |||
U251 (Fibroblasts or fibroblast like cells) | ||||
In-vivo Model | The male BALB/c nude mice were randomized divide into two groups, each group including six 4 weeks old nude mice. Investigators were blinded to the treatment groups during data collection and subsequent data analysis. In the subcutaneous xenograft model, 5 × 105 cells were subcutaneously injected in the right flanks of nude mice. In the orthotopic intracranial mouse model, each mouse was intracranially injected with 1 × 105 luciferase transfected U87MG cells in 10 uL PBS solution. | |||
YTH domain-containing protein 2 (YTHDC2) [READER]
In total 1 item(s) under this regulator | ||||
Experiment 1 Reporting the m6A Methylation Regulator of This Target Gene | [6] | |||
Response Summary | YTHDC2 destabilized Cystine/glutamate transporter (SLC7A11) mRNA in an m6A-dependent manner because YTHDC2 preferentially bound to m6A-modified SLC7A11 mRNA and thereafter promoted its decay. the promotion of cystine uptake via the suppression of YTHDC2 is critical for LUAD tumorigenesis | |||
Target Regulation | Down regulation | |||
Responsed Disease | Lung adenocarcinoma | ICD-11: 2C25.0 | ||
In-vitro Model | PC-9 | Lung adenocarcinoma | Homo sapiens | CVCL_B260 |
NCI-H441 | Lung papillary adenocarcinoma | Homo sapiens | CVCL_1561 | |
NCI-H292 | Lung mucoepidermoid carcinoma | Homo sapiens | CVCL_0455 | |
NCI-H1975 | Lung adenocarcinoma | Homo sapiens | CVCL_1511 | |
NCI-H1650 | Minimally invasive lung adenocarcinoma | Homo sapiens | CVCL_1483 | |
NCI-H1299 | Lung large cell carcinoma | Homo sapiens | CVCL_0060 | |
HEK293T | Normal | Homo sapiens | CVCL_0063 | |
HCC827 | Lung adenocarcinoma | Homo sapiens | CVCL_2063 | |
Calu-1 | Lung squamous cell carcinoma | Homo sapiens | CVCL_0608 | |
BEAS-2B | Normal | Homo sapiens | CVCL_0168 | |
A-549 | Lung adenocarcinoma | Homo sapiens | CVCL_0023 | |
Brain cancer [ICD-11: 2A00]
In total 1 item(s) under this disease | ||||
Experiment 1 Reporting the m6A-centered Disease Response | [5] | |||
Response Summary | This study NKAP knockdown induced cell death in glioblastoma cells. NKAP acted as a new ferroptosis suppressor by binding to m6A and then promoting Cystine/glutamate transporter (SLC7A11) mRNA splicing and maturation. | |||
Responsed Disease | Glioblastoma [ICD-11: 2A00.00] | |||
Target Regulator | NF-kappa-B-activating protein (NKAP) | READER | ||
Target Regulation | Up regulation | |||
Pathway Response | Ferroptosis | hsa04216 | ||
Cell Process | Ferroptosis | |||
In-vitro Model | U87MG (Astroblastoma cells from human brain) | |||
U251 (Fibroblasts or fibroblast like cells) | ||||
In-vivo Model | The male BALB/c nude mice were randomized divide into two groups, each group including six 4 weeks old nude mice. Investigators were blinded to the treatment groups during data collection and subsequent data analysis. In the subcutaneous xenograft model, 5 × 105 cells were subcutaneously injected in the right flanks of nude mice. In the orthotopic intracranial mouse model, each mouse was intracranially injected with 1 × 105 luciferase transfected U87MG cells in 10 uL PBS solution. | |||
Liver cancer [ICD-11: 2C12]
In total 3 item(s) under this disease | ||||
Experiment 1 Reporting the m6A-centered Disease Response | [1] | |||
Response Summary | METTL3-mediated Cystine/glutamate transporter (SLC7A11) m6A modification enhances hepatoblastoma ferroptosis resistance. The METTL3/IGF2BP1/m6A modification promotes SLC7A11 mRNA stability and upregulates its expression by inhibiting the deadenylation process. | |||
Responsed Disease | Hepatoblastoma [ICD-11: 2C12.01] | |||
Target Regulator | Insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1) | READER | ||
Target Regulation | Up regulation | |||
Pathway Response | Ferroptosis | hsa04216 | ||
Cell Process | Ferroptosis | |||
In-vitro Model | HuH-6 | Hepatoblastoma | Homo sapiens | CVCL_4381 |
Hep-G2 | Hepatoblastoma | Homo sapiens | CVCL_0027 | |
HEK293T | Normal | Homo sapiens | CVCL_0063 | |
Experiment 2 Reporting the m6A-centered Disease Response | [2] | |||
Response Summary | METTL14 induced m6A modification at 5'UTR of Cystine/glutamate transporter (SLC7A11) mRNA, which in turn underwent degradation relied on the YTHDF2-dependent pathway. Identify the HIF-1alpha /METTL14/YTHDF2/SLC7A11 axis as a potential therapeutic target for the HCC interventional embolization treatment. | |||
Responsed Disease | Liver cancer [ICD-11: 2C12] | |||
Target Regulator | Methyltransferase-like 14 (METTL14) | WRITER | ||
Target Regulation | Down regulation | |||
Pathway Response | HIF-1 signaling pathway | hsa04066 | ||
Cell Process | RNA stability | |||
In-vitro Model | PLC/PRF/5 | Adult hepatocellular carcinoma | Homo sapiens | CVCL_0485 |
MHCC97-H | Adult hepatocellular carcinoma | Homo sapiens | CVCL_4972 | |
L-02 | Endocervical adenocarcinoma | Homo sapiens | CVCL_6926 | |
Huh-7 | Adult hepatocellular carcinoma | Homo sapiens | CVCL_0336 | |
Hep-G2 | Hepatoblastoma | Homo sapiens | CVCL_0027 | |
HCCLM3 | Adult hepatocellular carcinoma | Homo sapiens | CVCL_6832 | |
BEL-7402 | Endocervical adenocarcinoma | Homo sapiens | CVCL_5492 | |
7721 (Human hepatic malignant cell line) | ||||
In-vivo Model | For the subcutaneous implantation model, 5 × 105 stable SLC7A11-knockdown HCCLM3 cells or SLC7A11-vector cells were injected subcutaneously into BALB/C nude mice. | |||
Experiment 3 Reporting the m6A-centered Disease Response | [1] | |||
Response Summary | METTL3-mediated Cystine/glutamate transporter (SLC7A11) m6A modification enhances hepatoblastoma ferroptosis resistance. The METTL3/IGF2BP1/m6A modification promotes SLC7A11 mRNA stability and upregulates its expression by inhibiting the deadenylation process. | |||
Responsed Disease | Hepatoblastoma [ICD-11: 2C12.01] | |||
Target Regulator | Methyltransferase-like 3 (METTL3) | WRITER | ||
Target Regulation | Up regulation | |||
Pathway Response | Ferroptosis | hsa04216 | ||
Cell Process | Ferroptosis | |||
In-vitro Model | HuH-6 | Hepatoblastoma | Homo sapiens | CVCL_4381 |
Hep-G2 | Hepatoblastoma | Homo sapiens | CVCL_0027 | |
HEK293T | Normal | Homo sapiens | CVCL_0063 | |
Lung cancer [ICD-11: 2C25]
In total 2 item(s) under this disease | ||||
Experiment 1 Reporting the m6A-centered Disease Response | [4] | |||
Response Summary | METTL3-mediated m-6A modification could stabilize Cystine/glutamate transporter (SLC7A11) mRNA and promote its translation, thus promoting LUAD cell proliferation and inhibiting cell ferroptosis, a novel form of programmed cell death. | |||
Responsed Disease | Lung adenocarcinoma [ICD-11: 2C25.0] | |||
Target Regulator | Methyltransferase-like 3 (METTL3) | WRITER | ||
Target Regulation | Up regulation | |||
Pathway Response | Ferroptosis | hsa04216 | ||
Cell Process | Ferroptosis | |||
In-vitro Model | SPC-A1 | Endocervical adenocarcinoma | Homo sapiens | CVCL_6955 |
PC-9 | Lung adenocarcinoma | Homo sapiens | CVCL_B260 | |
NCI-H460 | Lung large cell carcinoma | Homo sapiens | CVCL_0459 | |
NCI-H322 | Minimally invasive lung adenocarcinoma | Homo sapiens | CVCL_1556 | |
NCI-H1975 | Lung adenocarcinoma | Homo sapiens | CVCL_1511 | |
NCI-H1299 | Lung large cell carcinoma | Homo sapiens | CVCL_0060 | |
BEAS-2B | Normal | Homo sapiens | CVCL_0168 | |
A-549 | Lung adenocarcinoma | Homo sapiens | CVCL_0023 | |
In-vivo Model | For the subcutaneous xenograft model, PC9 cells stably transfected with METTL3 knockdown (shMETTL3) or negative control (shNC) shRNA (5 × 106 cells per mouse, n = 6) were suspended in 200 uL PBS with 50% Matrigel matrix (Corning, USA, 354234) and then injected into one side of the axilla of nude mice. | |||
Experiment 2 Reporting the m6A-centered Disease Response | [6] | |||
Response Summary | YTHDC2 destabilized Cystine/glutamate transporter (SLC7A11) mRNA in an m6A-dependent manner because YTHDC2 preferentially bound to m6A-modified SLC7A11 mRNA and thereafter promoted its decay. the promotion of cystine uptake via the suppression of YTHDC2 is critical for LUAD tumorigenesis | |||
Responsed Disease | Lung adenocarcinoma [ICD-11: 2C25.0] | |||
Target Regulator | YTH domain-containing protein 2 (YTHDC2) | READER | ||
Target Regulation | Down regulation | |||
In-vitro Model | PC-9 | Lung adenocarcinoma | Homo sapiens | CVCL_B260 |
NCI-H441 | Lung papillary adenocarcinoma | Homo sapiens | CVCL_1561 | |
NCI-H292 | Lung mucoepidermoid carcinoma | Homo sapiens | CVCL_0455 | |
NCI-H1975 | Lung adenocarcinoma | Homo sapiens | CVCL_1511 | |
NCI-H1650 | Minimally invasive lung adenocarcinoma | Homo sapiens | CVCL_1483 | |
NCI-H1299 | Lung large cell carcinoma | Homo sapiens | CVCL_0060 | |
HEK293T | Normal | Homo sapiens | CVCL_0063 | |
HCC827 | Lung adenocarcinoma | Homo sapiens | CVCL_2063 | |
Calu-1 | Lung squamous cell carcinoma | Homo sapiens | CVCL_0608 | |
BEAS-2B | Normal | Homo sapiens | CVCL_0168 | |
A-549 | Lung adenocarcinoma | Homo sapiens | CVCL_0023 | |
Breast cancer [ICD-11: 2C60]
In total 3 item(s) under this disease | ||||
Experiment 1 Reporting the m6A-centered Disease Response | [3] | |||
Response Summary | m6A-hypomethylation regulated FGFR4 phosphorylates GSK-3beta and activates beta-catenin/TCF4-Cystine/glutamate transporter (SLC7A11)/FPN1 signaling to drive anti-HER2 resistance. Knockdown of METTL14 significantly increased the expression level of FGFR4 in HER2-positive breast cancer cells. FGFR4 reduced the sensitivity of HER2-positive breast cancer to trastuzumab plus pertuzumab or tucatinib. These results pinpoint a mechanism of anti-HER2 resistance and provide a strategy for overcoming resistance via FGFR4 inhibition in recalcitrant HER2-positive breast cancer. | |||
Responsed Disease | Breast cancer [ICD-11: 2C60] | |||
Target Regulator | Methyltransferase-like 14 (METTL14) | WRITER | ||
Target Regulation | Down regulation | |||
Responsed Drug | Pertuzumab | Approved | ||
Pathway Response | Wnt signaling pathway | hsa04310 | ||
Cell Process | Glutathione synthesis | |||
In-vitro Model | ZR-75-1 | Invasive breast carcinoma | Homo sapiens | CVCL_0588 |
T-47D | Invasive breast carcinoma | Homo sapiens | CVCL_0553 | |
SUM-159 (A mesenchymal triple-negative breast cancer cell line) | ||||
SK-BR-3 | Breast adenocarcinoma | Homo sapiens | CVCL_0033 | |
MDA-MB-468 | Breast adenocarcinoma | Homo sapiens | CVCL_0419 | |
MDA-MB-453 | Breast adenocarcinoma | Homo sapiens | CVCL_0418 | |
MDA-MB-361 | Breast adenocarcinoma | Homo sapiens | CVCL_0620 | |
MDA-MB-231 | Breast adenocarcinoma | Homo sapiens | CVCL_0062 | |
MCF-7 | Invasive breast carcinoma | Homo sapiens | CVCL_0031 | |
MCF-10A | Normal | Homo sapiens | CVCL_0598 | |
HEK293T | Normal | Homo sapiens | CVCL_0063 | |
BT-549 | Invasive breast carcinoma | Homo sapiens | CVCL_1092 | |
BT-474 | Invasive breast carcinoma | Homo sapiens | CVCL_0179 | |
AU565 | Breast adenocarcinoma | Homo sapiens | CVCL_1074 | |
In-vivo Model | Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs. | |||
Experiment 2 Reporting the m6A-centered Disease Response | [3] | |||
Response Summary | m6A-hypomethylation regulated FGFR4 phosphorylates GSK-3beta and activates beta-catenin/TCF4-Cystine/glutamate transporter (SLC7A11)/FPN1 signaling to drive anti-HER2 resistance. Knockdown of METTL14 significantly increased the expression level of FGFR4 in HER2-positive breast cancer cells. FGFR4 reduced the sensitivity of HER2-positive breast cancer to trastuzumab plus pertuzumab or tucatinib. These results pinpoint a mechanism of anti-HER2 resistance and provide a strategy for overcoming resistance via FGFR4 inhibition in recalcitrant HER2-positive breast cancer. | |||
Responsed Disease | Breast cancer [ICD-11: 2C60] | |||
Target Regulator | Methyltransferase-like 14 (METTL14) | WRITER | ||
Target Regulation | Down regulation | |||
Responsed Drug | Trastuzumab | Approved | ||
Pathway Response | Wnt signaling pathway | hsa04310 | ||
Cell Process | Glutathione synthesis | |||
In-vitro Model | ZR-75-1 | Invasive breast carcinoma | Homo sapiens | CVCL_0588 |
T-47D | Invasive breast carcinoma | Homo sapiens | CVCL_0553 | |
SUM-159 (A mesenchymal triple-negative breast cancer cell line) | ||||
SK-BR-3 | Breast adenocarcinoma | Homo sapiens | CVCL_0033 | |
MDA-MB-468 | Breast adenocarcinoma | Homo sapiens | CVCL_0419 | |
MDA-MB-453 | Breast adenocarcinoma | Homo sapiens | CVCL_0418 | |
MDA-MB-361 | Breast adenocarcinoma | Homo sapiens | CVCL_0620 | |
MDA-MB-231 | Breast adenocarcinoma | Homo sapiens | CVCL_0062 | |
MCF-7 | Invasive breast carcinoma | Homo sapiens | CVCL_0031 | |
MCF-10A | Normal | Homo sapiens | CVCL_0598 | |
HEK293T | Normal | Homo sapiens | CVCL_0063 | |
BT-549 | Invasive breast carcinoma | Homo sapiens | CVCL_1092 | |
BT-474 | Invasive breast carcinoma | Homo sapiens | CVCL_0179 | |
AU565 | Breast adenocarcinoma | Homo sapiens | CVCL_1074 | |
In-vivo Model | Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs. | |||
Experiment 3 Reporting the m6A-centered Disease Response | [3] | |||
Response Summary | m6A-hypomethylation regulated FGFR4 phosphorylates GSK-3beta and activates beta-catenin/TCF4-Cystine/glutamate transporter (SLC7A11)/FPN1 signaling to drive anti-HER2 resistance. Knockdown of METTL14 significantly increased the expression level of FGFR4 in HER2-positive breast cancer cells. FGFR4 reduced the sensitivity of HER2-positive breast cancer to trastuzumab plus pertuzumab or tucatinib. These results pinpoint a mechanism of anti-HER2 resistance and provide a strategy for overcoming resistance via FGFR4 inhibition in recalcitrant HER2-positive breast cancer. | |||
Responsed Disease | Breast cancer [ICD-11: 2C60] | |||
Target Regulator | Methyltransferase-like 14 (METTL14) | WRITER | ||
Target Regulation | Down regulation | |||
Responsed Drug | Tucatinib | Approved | ||
Pathway Response | Wnt signaling pathway | hsa04310 | ||
Cell Process | Glutathione synthesis | |||
In-vitro Model | ZR-75-1 | Invasive breast carcinoma | Homo sapiens | CVCL_0588 |
T-47D | Invasive breast carcinoma | Homo sapiens | CVCL_0553 | |
SUM-159 (A mesenchymal triple-negative breast cancer cell line) | ||||
SK-BR-3 | Breast adenocarcinoma | Homo sapiens | CVCL_0033 | |
MDA-MB-468 | Breast adenocarcinoma | Homo sapiens | CVCL_0419 | |
MDA-MB-453 | Breast adenocarcinoma | Homo sapiens | CVCL_0418 | |
MDA-MB-361 | Breast adenocarcinoma | Homo sapiens | CVCL_0620 | |
MDA-MB-231 | Breast adenocarcinoma | Homo sapiens | CVCL_0062 | |
MCF-7 | Invasive breast carcinoma | Homo sapiens | CVCL_0031 | |
MCF-10A | Normal | Homo sapiens | CVCL_0598 | |
HEK293T | Normal | Homo sapiens | CVCL_0063 | |
BT-549 | Invasive breast carcinoma | Homo sapiens | CVCL_1092 | |
BT-474 | Invasive breast carcinoma | Homo sapiens | CVCL_0179 | |
AU565 | Breast adenocarcinoma | Homo sapiens | CVCL_1074 | |
In-vivo Model | Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs. | |||
Pertuzumab
[Approved]
In total 1 item(s) under this drug | ||||
Experiment 1 Reporting the m6A-centered Drug Response | [3] | |||
Response Summary | m6A-hypomethylation regulated FGFR4 phosphorylates GSK-3beta and activates beta-catenin/TCF4-Cystine/glutamate transporter (SLC7A11)/FPN1 signaling to drive anti-HER2 resistance. Knockdown of METTL14 significantly increased the expression level of FGFR4 in HER2-positive breast cancer cells. FGFR4 reduced the sensitivity of HER2-positive breast cancer to trastuzumab plus pertuzumab or tucatinib. These results pinpoint a mechanism of anti-HER2 resistance and provide a strategy for overcoming resistance via FGFR4 inhibition in recalcitrant HER2-positive breast cancer. | |||
Target Regulator | Methyltransferase-like 14 (METTL14) | WRITER | ||
Target Regulation | Down regulation | |||
Responsed Disease | Breast cancer | ICD-11: 2C60 | ||
Pathway Response | Wnt signaling pathway | hsa04310 | ||
Cell Process | Glutathione synthesis | |||
In-vitro Model | ZR-75-1 | Invasive breast carcinoma | Homo sapiens | CVCL_0588 |
T-47D | Invasive breast carcinoma | Homo sapiens | CVCL_0553 | |
SUM-159 (A mesenchymal triple-negative breast cancer cell line) | ||||
SK-BR-3 | Breast adenocarcinoma | Homo sapiens | CVCL_0033 | |
MDA-MB-468 | Breast adenocarcinoma | Homo sapiens | CVCL_0419 | |
MDA-MB-453 | Breast adenocarcinoma | Homo sapiens | CVCL_0418 | |
MDA-MB-361 | Breast adenocarcinoma | Homo sapiens | CVCL_0620 | |
MDA-MB-231 | Breast adenocarcinoma | Homo sapiens | CVCL_0062 | |
MCF-7 | Invasive breast carcinoma | Homo sapiens | CVCL_0031 | |
MCF-10A | Normal | Homo sapiens | CVCL_0598 | |
HEK293T | Normal | Homo sapiens | CVCL_0063 | |
BT-549 | Invasive breast carcinoma | Homo sapiens | CVCL_1092 | |
BT-474 | Invasive breast carcinoma | Homo sapiens | CVCL_0179 | |
AU565 | Breast adenocarcinoma | Homo sapiens | CVCL_1074 | |
In-vivo Model | Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs. | |||
Trastuzumab
[Approved]
In total 1 item(s) under this drug | ||||
Experiment 1 Reporting the m6A-centered Drug Response | [3] | |||
Response Summary | m6A-hypomethylation regulated FGFR4 phosphorylates GSK-3beta and activates beta-catenin/TCF4-Cystine/glutamate transporter (SLC7A11)/FPN1 signaling to drive anti-HER2 resistance. Knockdown of METTL14 significantly increased the expression level of FGFR4 in HER2-positive breast cancer cells. FGFR4 reduced the sensitivity of HER2-positive breast cancer to trastuzumab plus pertuzumab or tucatinib. These results pinpoint a mechanism of anti-HER2 resistance and provide a strategy for overcoming resistance via FGFR4 inhibition in recalcitrant HER2-positive breast cancer. | |||
Target Regulator | Methyltransferase-like 14 (METTL14) | WRITER | ||
Target Regulation | Down regulation | |||
Responsed Disease | Breast cancer | ICD-11: 2C60 | ||
Pathway Response | Wnt signaling pathway | hsa04310 | ||
Cell Process | Glutathione synthesis | |||
In-vitro Model | ZR-75-1 | Invasive breast carcinoma | Homo sapiens | CVCL_0588 |
T-47D | Invasive breast carcinoma | Homo sapiens | CVCL_0553 | |
SUM-159 (A mesenchymal triple-negative breast cancer cell line) | ||||
SK-BR-3 | Breast adenocarcinoma | Homo sapiens | CVCL_0033 | |
MDA-MB-468 | Breast adenocarcinoma | Homo sapiens | CVCL_0419 | |
MDA-MB-453 | Breast adenocarcinoma | Homo sapiens | CVCL_0418 | |
MDA-MB-361 | Breast adenocarcinoma | Homo sapiens | CVCL_0620 | |
MDA-MB-231 | Breast adenocarcinoma | Homo sapiens | CVCL_0062 | |
MCF-7 | Invasive breast carcinoma | Homo sapiens | CVCL_0031 | |
MCF-10A | Normal | Homo sapiens | CVCL_0598 | |
HEK293T | Normal | Homo sapiens | CVCL_0063 | |
BT-549 | Invasive breast carcinoma | Homo sapiens | CVCL_1092 | |
BT-474 | Invasive breast carcinoma | Homo sapiens | CVCL_0179 | |
AU565 | Breast adenocarcinoma | Homo sapiens | CVCL_1074 | |
In-vivo Model | Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs. | |||
Tucatinib
[Approved]
In total 1 item(s) under this drug | ||||
Experiment 1 Reporting the m6A-centered Drug Response | [3] | |||
Response Summary | m6A-hypomethylation regulated FGFR4 phosphorylates GSK-3beta and activates beta-catenin/TCF4-Cystine/glutamate transporter (SLC7A11)/FPN1 signaling to drive anti-HER2 resistance. Knockdown of METTL14 significantly increased the expression level of FGFR4 in HER2-positive breast cancer cells. FGFR4 reduced the sensitivity of HER2-positive breast cancer to trastuzumab plus pertuzumab or tucatinib. These results pinpoint a mechanism of anti-HER2 resistance and provide a strategy for overcoming resistance via FGFR4 inhibition in recalcitrant HER2-positive breast cancer. | |||
Target Regulator | Methyltransferase-like 14 (METTL14) | WRITER | ||
Target Regulation | Down regulation | |||
Responsed Disease | Breast cancer | ICD-11: 2C60 | ||
Pathway Response | Wnt signaling pathway | hsa04310 | ||
Cell Process | Glutathione synthesis | |||
In-vitro Model | ZR-75-1 | Invasive breast carcinoma | Homo sapiens | CVCL_0588 |
T-47D | Invasive breast carcinoma | Homo sapiens | CVCL_0553 | |
SUM-159 (A mesenchymal triple-negative breast cancer cell line) | ||||
SK-BR-3 | Breast adenocarcinoma | Homo sapiens | CVCL_0033 | |
MDA-MB-468 | Breast adenocarcinoma | Homo sapiens | CVCL_0419 | |
MDA-MB-453 | Breast adenocarcinoma | Homo sapiens | CVCL_0418 | |
MDA-MB-361 | Breast adenocarcinoma | Homo sapiens | CVCL_0620 | |
MDA-MB-231 | Breast adenocarcinoma | Homo sapiens | CVCL_0062 | |
MCF-7 | Invasive breast carcinoma | Homo sapiens | CVCL_0031 | |
MCF-10A | Normal | Homo sapiens | CVCL_0598 | |
HEK293T | Normal | Homo sapiens | CVCL_0063 | |
BT-549 | Invasive breast carcinoma | Homo sapiens | CVCL_1092 | |
BT-474 | Invasive breast carcinoma | Homo sapiens | CVCL_0179 | |
AU565 | Breast adenocarcinoma | Homo sapiens | CVCL_1074 | |
In-vivo Model | Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs. | |||
References