m6A Target Gene Information
General Information of the m6A Target Gene (ID: M6ATAR00130)
Full List of m6A Methylation Regulator of This Target Gene and Corresponding Disease/Drug Response(s)
MIR221
can be regulated by the following regulator(s), and cause disease/drug response(s). You can browse detail information of regulator(s) or disease/drug response(s).
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Methyltransferase-like 3 (METTL3) [WRITER]
| Representative RNA-seq result indicating the expression of this target gene regulated by METTL3 | ||
| Cell Line | DKO-1 cell line | Homo sapiens |
|
Treatment: METTL3 knockdown DKO-1 cell
Control: DKO-1 cell
|
GSE182382 | |
| Regulation |
  |
logFC: 2.60E+00 p-value: 1.63E-02 |
| More Results | Click to View More RNA-seq Results | |
| In total 2 item(s) under this regulator | ||||
| Experiment 1 Reporting the m6A Methylation Regulator of This Target Gene | [1] | |||
| Response Summary | METTL3 has an oncogenic role in bladder cancer through interacting with the microprocessor protein DGCR8 and positively modulating the microRNA 221 (MIR221) process in an m6A-dependent manner. | |||
| Target Regulation | Up regulation | |||
| Responsed Disease | Bladder cancer | ICD-11: 2C94 | ||
| Cell Process | Cell proliferation | |||
| In-vitro Model | EJ (Human bladder cancer cells) | |||
| T24 | Bladder carcinoma | Homo sapiens | CVCL_0554 | |
| In-vivo Model | About 1× 107 cells were injected subcutaneously into the axilla of the female athymic BALB/C nude mice (4-6 weeks old, 18-22 g, five mice per group). | |||
| Experiment 2 Reporting the m6A Methylation Regulator of This Target Gene | [2] | |||
| Response Summary | METTL3 positively modulates the pri-miR-221/222 maturation process in an m6A-dependent manner and subsequently activates Wnt/Beta-catenin signaling by inhibiting DKK2, thus promoting Ang-II-induced cardiac hypertrophy. | |||
| Target Regulation | Up regulation | |||
| Responsed Disease | Cardiomegaly | ICD-11: BC45 | ||
| Pathway Response | Wnt signaling pathway | hsa04310 | ||
| In-vitro Model | NRCMs (Primary neonatal rat cardiomyocytes (NRCMs)NRCMs were prepared from the hearts of 2- to 3-day-old SD rats according to the following protocol) | |||
| In-vivo Model | The pump was prefilled with Ang-II or saline and then incubated in sterile saline at 37 ℃ for 48 h. After the mice were anesthetized with 3.0% isoflurane mixed with oxygen, an incision was made on the back skin of the mice, and the pump was implanted into the subcutaneous area, followed by suturing the incision. After the operation, the mice were given buprenorphine (0.1 mg/kg) to reach analgesia. Finally, the regaining consciousness mice were returned to cages and fed until the end of the experiment. | |||
Bladder cancer [ICD-11: 2C94]
| In total 1 item(s) under this disease | ||||
| Experiment 1 Reporting the m6A-centered Disease Response | [1] | |||
| Response Summary | METTL3 has an oncogenic role in bladder cancer through interacting with the microprocessor protein DGCR8 and positively modulating the microRNA 221 (MIR221) process in an m6A-dependent manner. | |||
| Responsed Disease | Bladder cancer [ICD-11: 2C94] | |||
| Target Regulator | Methyltransferase-like 3 (METTL3) | WRITER | ||
| Target Regulation | Up regulation | |||
| Cell Process | Cell proliferation | |||
| In-vitro Model | EJ (Human bladder cancer cells) | |||
| T24 | Bladder carcinoma | Homo sapiens | CVCL_0554 | |
| In-vivo Model | About 1× 107 cells were injected subcutaneously into the axilla of the female athymic BALB/C nude mice (4-6 weeks old, 18-22 g, five mice per group). | |||
Cardiomegaly [ICD-11: BC45]
| In total 1 item(s) under this disease | ||||
| Experiment 1 Reporting the m6A-centered Disease Response | [2] | |||
| Response Summary | METTL3 positively modulates the pri-miR-221/222 maturation process in an m6A-dependent manner and subsequently activates Wnt/Beta-catenin signaling by inhibiting DKK2, thus promoting Ang-II-induced cardiac hypertrophy. | |||
| Responsed Disease | Cardiomegaly [ICD-11: BC45] | |||
| Target Regulator | Methyltransferase-like 3 (METTL3) | WRITER | ||
| Target Regulation | Up regulation | |||
| Pathway Response | Wnt signaling pathway | hsa04310 | ||
| In-vitro Model | NRCMs (Primary neonatal rat cardiomyocytes (NRCMs)NRCMs were prepared from the hearts of 2- to 3-day-old SD rats according to the following protocol) | |||
| In-vivo Model | The pump was prefilled with Ang-II or saline and then incubated in sterile saline at 37 ℃ for 48 h. After the mice were anesthetized with 3.0% isoflurane mixed with oxygen, an incision was made on the back skin of the mice, and the pump was implanted into the subcutaneous area, followed by suturing the incision. After the operation, the mice were given buprenorphine (0.1 mg/kg) to reach analgesia. Finally, the regaining consciousness mice were returned to cages and fed until the end of the experiment. | |||
Full List of Crosstalk(s) between m6A Modification and Epigenetic Regulation Related to This Regulator
RNA modification
m6A Regulator: Methyltransferase-like 3 (METTL3)
| In total 3 item(s) under this m6A regulator | ||
| Crosstalk ID: M6ACROT00648 | ||
| Epigenetic Regulator | Y-box-binding protein 1 (YBX1) | |
| Regulated Target | Growth arrest specific 5 (GAS5) | |
| Crosstalk relationship | m5C → m6A | |
| Crosstalk ID: M6ACROT00649 | ||
| Epigenetic Regulator | Fat mass and obesity-associated protein (FTO) | |
| Regulated Target | NAD-dependent protein deacetylase sirtuin-1 (SIRT1) | |
| Crosstalk relationship | m6A → m6Am | |
| Crosstalk ID: M6ACROT00650 | ||
| Epigenetic Regulator | Fat mass and obesity-associated protein (FTO) | |
| Regulated Target | Protein c-Fos (FOS) | |
| Crosstalk relationship | m6A → m6Am | |
Non-coding RNA
m6A Regulator: Methyltransferase-like 3 (METTL3)
| In total 2 item(s) under this m6A regulator | ||
| Crosstalk ID: M6ACROT05381 | ||
| Epigenetic Regulator | MicroRNA 221 (MIR221) | |
| Regulated Target | Mutated in multiple advanced cancers 1 (PTEN) | |
| Crosstalk relationship | m6A → ncRNA | |
| Disease | Bladder cancer | |
| Crosstalk ID: M6ACROT05561 | ||
| Epigenetic Regulator | MicroRNA 221 (MIR221) | |
| Regulated Target | Dickkopf-related protein 2 (DKK2) | |
| Crosstalk relationship | m6A → ncRNA | |
| Disease | Cardiomegaly | |
References