m6A-centered Crosstalk Information

Mechanism of Crosstalk between m6A Modification and Epigenetic Regulation
Crosstalk ID
M6ACROT00648
 [1], [2], [3], [4], [5], [6]
RNA modification GAS5 GAS5 YBX1 : modification sites Indirect Enhancement m6A modification MIR221 MIR221 METTL3 Methylation : m6A sites
m6A Modification:
m6A Regulator Methyltransferase-like 3 (METTL3) WRITER
 Regulator Info 
m6A Target microRNA 221 (MIR221)
 Targert Gene 
Epigenetic Regulation that have Cross-talk with This m6A Modification:
Epigenetic Regulation Type RNA modification (RNAMod)  >> 5-methylcytidine (m5C)
Epigenetic Regulator Y-box-binding protein 1 (YBX1) READER View Details
Regulated Target Growth arrest specific 5 (GAS5) View Details
Crosstalk Relationship m5C  →  m6A Enhancement
Crosstalk Mechanism RNA modification indirectly impacts m6A modification through downstream signaling pathways
Crosstalk Summary YBX1 interacts with Growth arrest specific 5 (GAS5), reading its m5C sites and promoting its physical interaction with microRNA 221 (MIR221), which was regulated by METTL3-mediated m6A modification.
In-vitro Model
 HGC-27 Gastric carcinoma Homo sapiens CVCL_1279
T24 Bladder carcinoma Homo sapiens CVCL_0554
Ej138 Bladder carcinoma Homo sapiens CVCL_2443
MCF-7 Invasive breast carcinoma Homo sapiens CVCL_0031
References
Ref 1 lncRNA GAS5 enhances G1 cell cycle arrest via binding to YBX1 to regulate p21 expression in stomach cancer. Sci Rep. 2015 May 11;5:10159. doi: 10.1038/srep10159.
Ref 2 Long noncoding RNA GAS5 induces abdominal aortic aneurysm formation by promoting smooth muscle apoptosis. Theranostics. 2019 Jul 28;9(19):5558-5576. doi: 10.7150/thno.34463. eCollection 2019.
Ref 3 METTL3 promote tumor proliferation of bladder cancer by accelerating pri-miR221/222 maturation in m6A-dependent manner. Mol Cancer. 2019 Jun 22;18(1):110. doi: 10.1186/s12943-019-1036-9.
Ref 4 METTL3 promotes adriamycin resistance in MCF-7 breast cancer cells by accelerating pri-microRNA-221-3p maturation in a m6A-dependent manner. Exp Mol Med. 2021 Jan;53(1):91-102. doi: 10.1038/s12276-020-00510-w. Epub 2021 Jan 8.
Ref 5 Long noncoding RNA GAS5 inhibits cell proliferation and fibrosis in diabetic nephropathy by sponging miR-221 and modulating SIRT1 expression. Aging (Albany NY). 2019 Oct 20;11(20):8745-8759. doi: 10.18632/aging.102249. Epub 2019 Oct 20.
Ref 6 LncRNAGAS5 sponges miRNA-221 to promote neurons apoptosis by up-regulated PUMA under hypoxia condition. Neurol Res. 2020 Jan;42(1):8-16. doi: 10.1080/01616412.2019.1672382. Epub 2019 Dec 27.