m6A-centered Disease Response Information
General Information of the Disease (ID: M6ADIS0128)
| Name |
Female reproductive system disorders
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|---|---|---|---|---|---|
| ICD |
ICD-11: SC4Y
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Full List of Target Gene(s) of This m6A-centered Disease Response
Cellular tumor antigen p53 (TP53/p53)
| In total 1 item(s) under this target gene | ||||
| Experiment 1 Reporting the m6A-centered Disease Response by This Target Gene | [1] | |||
| Response Summary | ALKBH5 as another mammalian demethylase that oxidatively reverses m(6)A in mRNA in vitro and in vivo. Alkbh5-deficient male mice have increased m(6)A in mRNA and are characterized by impaired fertility resulting from apoptosis that affects meiotic metaphase-stage spermatocytes. Identified in mouse testes 1,551 differentially expressed genes that cover broad functional categories and include spermatogenesis-related mRNAs involved in the Cellular tumor antigen p53 (TP53/p53) functional interaction network. | |||
| Responsed Disease | Female reproductive system disorders [ICD-11: SC4Y] | |||
| Target Regulator | RNA demethylase ALKBH5 (ALKBH5) | ERASER | ||
| Pathway Response | p53 signaling pathway | hsa04115 | ||
| In-vitro Model | HeLa | Endocervical adenocarcinoma | Homo sapiens | CVCL_0030 |
| In-vivo Model | Neo-resistant recombinant ESCs were analyzed for correct 3' and 5' targeting through hybridization by standard protocols, and we identified seven correctly targeted clones. Following ESC cell injection, several high-percentage chimera males were born. These were bred with Cre-expressing mice, and Cre-mediated excision of Alkbh5 exon 1 was tested in 26 agouti F1 pups. Two animals tested positive for the excised allele. These two mice, heterozygous for the constitutive allele (Alkbh5+/-), were analyzed by Southern blot analysis and used for further breeding to generate Alkbh5-/- mice. | |||
Circ_GFR-Alpha-1
| In total 1 item(s) under this target gene | ||||
| Experiment 1 Reporting the m6A-centered Disease Response by This Target Gene | [2] | |||
| Response Summary | Circ_GFR-Alpha-1 promotes female germline stem cells self-renewal by acting as a ceRNA that sponges miR-449, leading to enhanced GFRalpha-1 expression and activation of the GDNF signaling pathway. circGFRalpha-1 acts as a ceRNA based on METTL14-mediated cytoplasmic export through the GGACU motif. | |||
| Responsed Disease | Female reproductive system disorders [ICD-11: SC4Y] | |||
| Target Regulator | Methyltransferase-like 14 (METTL14) | WRITER | ||
| Pathway Response | Calcium signaling pathway | hsa04020 | ||
| In-vitro Model | HEK293T | Normal | Homo sapiens | CVCL_0063 |
| FGSCs cell line (Female Germline Stem Cells) | ||||
Full List of Crosstalk(s) between m6A Modification and Epigenetic Regulation Related to This Disease
| In total 1 item(s) under this disease | ||
| Crosstalk ID: M6ACROT05491 | ||
| m6A Regulator | Methyltransferase-like 14 (METTL14) | |
| m6A Target | Circ_GFR-Alpha-1 | |
| Epigenetic Regulator | Circ_GFR-Alpha-1 | |
| Regulated Target | MicroRNA 449a (MIR449A) | |
| Crosstalk relationship | m6A → ncRNA | |
References