m6A-centered Crosstalk Information

Mechanism of Crosstalk between m6A Modification and Epigenetic Regulation
Crosstalk ID
M6ACROT05691
 [1]
m6A modification miR-92b-5p miR-92b-5p WTAP Methylation : m6A sites Direct Inhibition Non-coding RNA miR-92b-5p TIMP4  lncRNA       miRNA   circRNA
m6A Modification:
m6A Regulator Wilms tumor 1-associating protein (WTAP) WRITER
 Regulator Info 
m6A Target hsa-miR-92b-5p
 Targert Gene 
Epigenetic Regulation that have Cross-talk with This m6A Modification:
Epigenetic Regulation Type Non-coding RNA (ncRNA)
Epigenetic Regulator hsa-miR-92b-5p microRNA View Details
Regulated Target Metalloproteinase inhibitor 4 (TIMP4) View Details
Crosstalk Relationship m6A  →  ncRNA Inhibition
Crosstalk Mechanism m6A regulators directly modulate the functionality of ncRNAs through specific targeting ncRNA
Crosstalk Summary m6A modification mediated by WTAP promotes the maturation of pri-miR-92b to hsa-miR-92b-5p, thereby enhancing the targeted inhibitory function of miR-92b-5p on Metalloproteinase inhibitor 4 (TIMP4). Furthermore, we have discovered that WTAP can directly facilitate the degradation of TIMP4 mRNAs in a YTHDF2-dependent manner.
Responsed Disease Osteoarthritis ICD-11: FA05
 Disease Info 
Cell Process Cell apoptosis
Cell proliferation
In-vivo Model The male C57BL/6 mice (SPF, Eight-week-old) were randomly divided into four groups: sham (n = 6), model (n = 6), model + adeno-associated virus-negative control (AVV-shNC) (n = 6), and model + AVV-shWTAP groups (n = 6). After acclimating for one week, mice in other groups were treated with DMM surgery to induce OA as previously described except for the sham groups [19]. The mice in sham group were underwent only the skin of the right knee joint incision. The AAV-shNC and AAV-shWTAP were constructed by HANBIO (Shanghai, China). The AAV-shNC and AAV-shWTAP groups were intra-articularly injected with 10 μL of AAV-shNC and AAV-shWTAP (1 × 1013 vg/ml) through the medial parapatellar area at two weeks after the DMM operation, respectively.
References
Ref 1 N6-methyladenosine (m6A) methyltransferase WTAP-mediated miR-92b-5p accelerates osteoarthritis progression. Cell Commun Signal. 2023 Aug 10;21(1):199. doi: 10.1186/s12964-023-01228-8.