m6A-centered Crosstalk Information
Mechanism of Crosstalk between m6A Modification and Epigenetic Regulation
| Crosstalk ID |
M6ACROT05619
|
[1] | |||
m6A modification
AC008
AC008
FTO
Demethylation
 : m6A sites
Direct
Inhibition
Non-coding RNA
AC008
miR-328-3p
lncRNA miRNA circRNA
|
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| m6A Modification: | |||||
|---|---|---|---|---|---|
| m6A Regulator | Fat mass and obesity-associated protein (FTO) | ERASER | |||
| m6A Target | AC008440.5 (AC008) | ||||
| Epigenetic Regulation that have Cross-talk with This m6A Modification: | |||||
| Epigenetic Regulation Type | Non-coding RNA (ncRNA) | ||||
| Epigenetic Regulator | AC008440.5 (AC008) | LncRNA | View Details | ||
| Regulated Target | hsa-miR-328-3p | View Details | |||
| Crosstalk Relationship | m6A → ncRNA | Inhibition | |||
| Crosstalk Mechanism | m6A regulators directly modulate the functionality of ncRNAs through specific targeting ncRNA | ||||
| Crosstalk Summary | FTO-mediated N6-methyladenosine demethylation downregulated AC008440.5 (AC008) transcription, while lower FTO expression led to upregulation of AC008 transcription in OA. AC008 functioned as a competing endogenous RNA (ceRNA) to regulate hsa-miR-328-3p. | ||||
| Responsed Disease | Osteoarthritis | ICD-11: FA05 | |||
| Cell Process | Cell apoptosis | ||||
In-vitro Model |
Chondrocytes (Chondrocytes were isolated from human cartilage and cultured) | ||||
| In-vivo Model | 10-week-old male C57BL/6 mice were randomly divided into six groups: normal group (n = 5); MIA group (n = 5); MIA + agomir-NC group (n = 5); MIA + agomir-328-3p group (n = 5); MIA + AAV-NC group (n = 5); and MIA + AAV-AC008 group (n = 5). For induction of OA, mice were given an intra-articular injection of MIA (Sigma-Aldrich) in the knee. One week later, agomir-328-3p or negative control agomir (RiboBio) and adeno-associated virus expressing AC008 (AAV-AC008) or negative control virus (GeneChem, Shanghai, China) were injected into the knee joints of mice in the appropriate groups. Treatments were administered at 20 μL per joint per mouse once per week for 5 consecutive weeks. Six weeks later, the mice were euthanized, and the knee joints were collected for safranin-O/fast green staining and IHC analysis. | ||||