m6A-centered Crosstalk Information
Mechanism of Crosstalk between m6A Modification and Epigenetic Regulation
| Crosstalk ID |
M6ACROT05338
|
[1] | |||
Non-coding RNA
miR-27a-3p
FTO
lncRNA miRNA circRNA
Direct
Inhibition
m6A modification
FOXO3
FOXO3
FTO
Demethylation
 : m6A sites
|
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| m6A Modification: | |||||
|---|---|---|---|---|---|
| m6A Regulator | Fat mass and obesity-associated protein (FTO) | ERASER | |||
| m6A Target | Forkhead box protein O3 (FOXO3) | ||||
| Epigenetic Regulation that have Cross-talk with This m6A Modification: | |||||
| Epigenetic Regulation Type | Non-coding RNA (ncRNA) | ||||
| Epigenetic Regulator | hsa-miR-27a-3p | microRNA | View Details | ||
| Regulated Target | FTO alpha-ketoglutarate dependent dioxygenase (FTO) | View Details | |||
| Crosstalk Relationship | ncRNA → m6A | Inhibition | |||
| Crosstalk Mechanism | ncRNAs directly impacts m6A modification through modulating the expression level of m6A regulator | ||||
| Crosstalk Summary | miR-27a-3p inhibits the expression of FTO via direct binding to FTO. FTO overexpression promotes the nuclear translocation of Forkhead box protein O3 (FOXO3) and upregulates the expression levels of the FOXO3a downstream targets BIM, BNIP3, BCL-6, and PUMA, possibly by interacting with FOXO3a. | ||||
| Responsed Disease | Glioblastoma multiforme | ICD-11: XH0MB1 | |||
| In-vivo Model | GBM cells were infected with lentivirus overexpressing FTO (Lv-FTO) or control lentivirus (Lv-NC), followed by subcutaneous injection with 2.5×105 cells in a total volume of 200 μl into the right dorsal-lateral flanks of mice. | ||||