m6A-centered Crosstalk Information

Mechanism of Crosstalk between m6A Modification and Epigenetic Regulation

m6A Modification:
Crosstalk ID	M6ACROT00639				[1], [2], [3], [4], [5]
m⁶A modification MIR25 MIR25 METTL3 Methylation : m⁶A sites Indirect Enhancement RNA modification SNAI1 SNAI1 YBX1 : modification sites
m6A Regulator	Methyltransferase-like 3 (METTL3)			WRITER	Regulator Info
m6A Target	microRNA 25 (MIR25)				Targert Gene
Epigenetic Regulation that have Cross-talk with This m6A Modification:
Epigenetic Regulation Type	RNA modification (RNAMod) >> 5-methylcytidine (m5C)
Epigenetic Regulator	Y-box-binding protein 1 (YBX1)			READER	View Details
Regulated Target	Zinc finger protein SNAI1 (SNAI1)				View Details
Crosstalk Relationship	m6A → m5C			Enhancement
Crosstalk Mechanism	m6A modification indirectly impacts RNA modification through downstream signaling pathways
Crosstalk Summary	METTL3 interacts with microRNA 25 (MIR25), reading its m6A level and promoting its physical interaction with Zinc finger protein SNAI1 (SNAI1), which was regulated by YBX1-mediated m5C modification.
In-vitro Model	MDA-MB-435S	Amelanotic melanoma	Homo sapiens		CVCL_0622
	MDA-MB-231	Breast adenocarcinoma	Homo sapiens		CVCL_0062
	Huh-7	Adult hepatocellular carcinoma	Homo sapiens		CVCL_0336
	MDA-MB-231	Breast adenocarcinoma	Homo sapiens		CVCL_0062
	ARPE-19	Normal	Homo sapiens		CVCL_0145

References

Ref 1	Translational activation of snail1 and other developmentally regulated transcription factors by YB-1 promotes an epithelial-mesenchymal transition. Cancer Cell. 2009 May 5;15(5):402-15. doi: 10.1016/j.ccr.2009.03.017.
Ref 2	Prostaglandin E2 promotes hepatocellular carcinoma cell invasion through upregulation of YB-1 protein expression. Int J Oncol. 2014 Mar;44(3):769-80. doi: 10.3892/ijo.2013.2234. Epub 2013 Dec 30.
Ref 3	N6-methyladenosine marks primary microRNAs for processing. Nature. 2015 Mar 26;519(7544):482-5. doi: 10.1038/nature14281. Epub 2015 Mar 18.
Ref 4	Overexpression of METTL3 attenuates high-glucose induced RPE cell pyroptosis by regulating miR-25-3p/PTEN/Akt signaling cascade through DGCR8. Aging (Albany NY). 2020 May 4;12(9):8137-8150. doi: 10.18632/aging.103130. Epub 2020 May 4.
Ref 5	MicroRNA?25 protects nucleus pulposus cells against apoptosis via targeting SUMO2 in intervertebral disc degeneration. Mol Med Rep. 2021 Oct;24(4):724. doi: 10.3892/mmr.2021.12363. Epub 2021 Aug 13.