m6A-centered Crosstalk Information

Mechanism of Crosstalk between m6A Modification and Epigenetic Regulation

m6A Modification:
Crosstalk ID	M6ACROT00001				[1]
RNA modification CDKN1A CDKN1A NSUN2 Methylation : modification sites Direct Enhancement m⁶A modification CDKN1A CDKN1A METTL3 Methylation : m⁶A sites
m6A Regulator	Methyltransferase-like 3 (METTL3)			WRITER	Regulator Info
m6A Target	Cyclin-dependent kinase inhibitor 1 (CDKN1A)				Targert Gene
Epigenetic Regulation that have Cross-talk with This m6A Modification:
Epigenetic Regulation Type	RNA modification (RNAMod) >> 5-methylcytidine (m5C)
Epigenetic Regulator	RNA cytosine C(5)-methyltransferase NSUN2 (NSUN2)			WRITER	View Details
Regulated Target	Cyclin-dependent kinase inhibitor 1 (CDKN1A)				View Details
Crosstalk Relationship	m5C → m6A			Enhancement
Crosstalk Mechanism	RNA modification directly impacts m6A modification through targeting the shared RNA
Crosstalk Summary	NSUN2-mediated m5C and METTL3/METTL14-mediated m6A methylation synergistically enhance Cyclin-dependent kinase inhibitor 1 (CDKN1A) expression at the translational level, leading to elevated expression of p21 in oxidative stress-induced cellular senescence.
Cell Process	Cellular senescence
In-vitro Model	HCT 116 TP53(-/-)	Colon carcinoma	Homo sapiens		CVCL_HD97
In-vitro Model	HeLa	Endocervical adenocarcinoma	Homo sapiens		CVCL_0030

References

Ref 1	NSUN2-Mediated m5C Methylation and METTL3/METTL14-Mediated m6A Methylation Cooperatively Enhance p21 Translation. J Cell Biochem. 2017 Sep;118(9):2587-2598. doi: 10.1002/jcb.25957. Epub 2017 May 3.