m6A Target Gene Information

General Information of the m6A Target Gene (ID: M6ATAR00486)
Target Name Thrombospondin-1 (THBS1)
Synonyms
Glycoprotein G; TSP; TSP1
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Gene Name THBS1
Family thrombospondin family. {ECO:0000305}.
Function
Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp (By similarity). Ligand for CD36 mediating antiangiogenic properties. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors (By similarity). {ECO:0000250, ECO:0000269|PubMed:11134179, ECO:0000269|PubMed:15014436}.
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Gene ID 5395
Uniprot ID
TSP1_HUMAN
HGNC ID
HGNC:11785
Ensembl Gene ID
ENSG00000137801
KEGG ID
hsa:7057
Full List of m6A Methylation Regulator of This Target Gene and Corresponding Disease/Drug Response(s)
THBS1 can be regulated by the following regulator(s), and cause disease/drug response(s). You can browse detail information of regulator(s) or disease/drug response(s).
Browse Regulator
Browse Disease
YTH domain-containing family protein 2 (YTHDF2) [READER]
 Regulator Info 
Representative RNA-seq result indicating the expression of this target gene regulated by YTHDF2
Cell Line Human umbilical cord blood CD34+ cells Homo sapiens
Treatment: YTHDF2 knockdown UCB CD34+ cells
Control: Wild type UCB CD34+ cells
 GSE107956
Regulation
logFC: -9.06E-01
p-value: 2.97E-03
More Results Click to View More RNA-seq Results
Representative RIP-seq result supporting the interaction between THBS1 and the regulator
Cell Line Hela Homo sapiens
Regulation logFC: 2.48E+00 GSE49339
In total 1 item(s) under this regulator
Experiment 1 Reporting the m6A Methylation Regulator of This Target Gene [1]
Response Summary In prostate cancer, METTL14 downregulated Thrombospondin-1 (THBS1) expression in an m6A-dependent manner, which resulted in the recruitment of YTHDF2 to recognize and degrade Thrombospondin 1 (THBS1) mRNA.
Target Regulation Down regulation
Responsed Disease Prostate cancer ICD-11: 2C82
 Disease Info 
Pathway Response RNA degradation hsa03018
Cell Process Cell proliferation
In-vitro Model PC-3 Prostate carcinoma Homo sapiens CVCL_0035
DU145 Prostate carcinoma Homo sapiens CVCL_0105
In-vivo Model Stably transfected shMETTL14 and shNC DU145 cells (5×106 cells) suspended in a mixture of 100uL PBS were subcutaneously injected into the right flank of male nude BALB/C mice (6-8 weeks old) to induce tumor formation.
Insulin-like growth factor-binding protein 3 (IGFBP3) [READER]
 Regulator Info 
Representative RNA-seq result indicating the expression of this target gene regulated by IGFBP3
Cell Line SAS cell line Homo sapiens
Treatment: siIGFBP3 SAS cells
Control: siControl SAS cells
 GSE205275
Regulation
logFC: -1.19E+00
p-value: 1.58E-03
More Results Click to View More RNA-seq Results
In total 1 item(s) under this regulator
Experiment 1 Reporting the m6A Methylation Regulator of This Target Gene [2]
Response Summary IGFBP3 was shown to function as a suppressor of invasion in epithelial ovarian cancer (EOC). IGFBP3 could activate Thrombospondin-1 (THBS1) through promoter regulation mainly via an intracellular signaling pathway, such angiogenesis-regulating ability represents a major function of IGFBP3 as an onco-suppressor in the pathogenesis of ovarian cancer.
Responsed Disease Malignant mixed epithelial mesenchymal tumour of ovary ICD-11: 2B5D.0
 Disease Info 
Cell Process Cell proliferation
In-vitro Model OVTW59 (Ovarian cancer cell line)
OVTW59-P0 (Ovarian cancer cell line sub-lines)
OVTW59-P4 (Ovarian cancer cell line sub-lines)
A-549 Lung adenocarcinoma Homo sapiens CVCL_0023
NCI-H1299 Lung large cell carcinoma Homo sapiens CVCL_0060
HEK293T Normal Homo sapiens CVCL_0063
In-vivo Model Severe combined immunodeficiency (SCID) female mice aged 6-8 weeks were transplanted subcutaneously with 2 × 107 P4-pBIG2i-hIGFBP3 or P4-pBIG2i transfectant cells. Tumor growth was measured using Vernier calipers and the volume was calculated using the formula: length × width × width × 0.52, which approximates the volume of an elliptical solid mass. Doxycycline at 2 mg/mL in drinking water with 2% sucrose was used to feed the animals when the tumor size was over 0.5 cm in diameter. The xenograft tumors were removed from five animals from each group on days 4, 7, 11, 14, and 20 after doxycycline treatment.
Methyltransferase-like 14 (METTL14) [WRITER]
 Regulator Info 
Representative RNA-seq result indicating the expression of this target gene regulated by METTL14
Cell Line HepG2 cell line Homo sapiens
Treatment: shMETTL14 HepG2 cells
Control: shCtrl HepG2 cells
 GSE121949
Regulation
logFC: -1.33E+00
p-value: 7.54E-27
More Results Click to View More RNA-seq Results
In total 1 item(s) under this regulator
Experiment 1 Reporting the m6A Methylation Regulator of This Target Gene [1]
Response Summary In prostate cancer, METTL14 downregulated Thrombospondin-1 (THBS1) expression in an m6A-dependent manner, which resulted in the recruitment of YTHDF2 to recognize and degrade Thrombospondin 1 (THBS1) mRNA.
Target Regulation Down regulation
Responsed Disease Prostate cancer ICD-11: 2C82
 Disease Info 
Pathway Response RNA degradation hsa03018
Cell Process Cell proliferation
In-vitro Model PC-3 Prostate carcinoma Homo sapiens CVCL_0035
DU145 Prostate carcinoma Homo sapiens CVCL_0105
In-vivo Model Stably transfected shMETTL14 and shNC DU145 cells (5×106 cells) suspended in a mixture of 100uL PBS were subcutaneously injected into the right flank of male nude BALB/C mice (6-8 weeks old) to induce tumor formation.
Malignant mixed epithelial mesenchymal tumour [ICD-11: 2B5D]
 Disease Info 
In total 1 item(s) under this disease
Experiment 1 Reporting the m6A-centered Disease Response [2]
Response Summary IGFBP3 was shown to function as a suppressor of invasion in epithelial ovarian cancer (EOC). IGFBP3 could activate Thrombospondin-1 (THBS1) through promoter regulation mainly via an intracellular signaling pathway, such angiogenesis-regulating ability represents a major function of IGFBP3 as an onco-suppressor in the pathogenesis of ovarian cancer.
Responsed Disease Malignant mixed epithelial mesenchymal tumour of ovary [ICD-11: 2B5D.0]
Target Regulator Insulin-like growth factor-binding protein 3 (IGFBP3) READER
 Regulator Info 
Cell Process Cell proliferation
In-vitro Model OVTW59 (Ovarian cancer cell line)
OVTW59-P0 (Ovarian cancer cell line sub-lines)
OVTW59-P4 (Ovarian cancer cell line sub-lines)
A-549 Lung adenocarcinoma Homo sapiens CVCL_0023
NCI-H1299 Lung large cell carcinoma Homo sapiens CVCL_0060
HEK293T Normal Homo sapiens CVCL_0063
In-vivo Model Severe combined immunodeficiency (SCID) female mice aged 6-8 weeks were transplanted subcutaneously with 2 × 107 P4-pBIG2i-hIGFBP3 or P4-pBIG2i transfectant cells. Tumor growth was measured using Vernier calipers and the volume was calculated using the formula: length × width × width × 0.52, which approximates the volume of an elliptical solid mass. Doxycycline at 2 mg/mL in drinking water with 2% sucrose was used to feed the animals when the tumor size was over 0.5 cm in diameter. The xenograft tumors were removed from five animals from each group on days 4, 7, 11, 14, and 20 after doxycycline treatment.
Prostate cancer [ICD-11: 2C82]
 Disease Info 
In total 2 item(s) under this disease
Experiment 1 Reporting the m6A-centered Disease Response [1]
Response Summary In prostate cancer, METTL14 downregulated Thrombospondin-1 (THBS1) expression in an m6A-dependent manner, which resulted in the recruitment of YTHDF2 to recognize and degrade Thrombospondin 1 (THBS1) mRNA.
Responsed Disease Prostate cancer [ICD-11: 2C82]
Target Regulator Methyltransferase-like 14 (METTL14) WRITER
 Regulator Info 
Target Regulation Down regulation
Pathway Response RNA degradation hsa03018
Cell Process Cell proliferation
In-vitro Model PC-3 Prostate carcinoma Homo sapiens CVCL_0035
DU145 Prostate carcinoma Homo sapiens CVCL_0105
In-vivo Model Stably transfected shMETTL14 and shNC DU145 cells (5×106 cells) suspended in a mixture of 100uL PBS were subcutaneously injected into the right flank of male nude BALB/C mice (6-8 weeks old) to induce tumor formation.
Experiment 2 Reporting the m6A-centered Disease Response [1]
Response Summary In prostate cancer, METTL14 downregulated Thrombospondin-1 (THBS1) expression in an m6A-dependent manner, which resulted in the recruitment of YTHDF2 to recognize and degrade Thrombospondin 1 (THBS1) mRNA.
Responsed Disease Prostate cancer [ICD-11: 2C82]
Target Regulator YTH domain-containing family protein 2 (YTHDF2) READER
 Regulator Info 
Target Regulation Down regulation
Pathway Response RNA degradation hsa03018
Cell Process Cell proliferation
In-vitro Model PC-3 Prostate carcinoma Homo sapiens CVCL_0035
DU145 Prostate carcinoma Homo sapiens CVCL_0105
In-vivo Model Stably transfected shMETTL14 and shNC DU145 cells (5×106 cells) suspended in a mixture of 100uL PBS were subcutaneously injected into the right flank of male nude BALB/C mice (6-8 weeks old) to induce tumor formation.
References
Ref 1 METTL14 promotes prostate tumorigenesis by inhibiting THBS1 via an m6A-YTHDF2-dependent mechanism. Cell Death Discov. 2022 Mar 30;8(1):143. doi: 10.1038/s41420-022-00939-0.
Ref 2 IGFBP3 inhibits angiogenesis through intracellular regulation of THBS1 expression. Am J Cancer Res. 2020 Jun 1;10(6):1728-1744. eCollection 2020.