m6A-centered Disease Response Information

General Information of the Disease (ID: M6ADIS0151)
Name
Malignant neoplasms of tonsil
ICD
ICD-11: 2B69
Full List of Target Gene(s) of This m6A-centered Disease Response
Protein E6 (E6)
 Target Info 
In total 3 item(s) under this target gene
Experiment 1 Reporting the m6A-centered Disease Response by This Target Gene [1]
Response Summary Overexpression of the ALKBH5 promoted production of intron retention on the human papillomavirus type 16 (HPV16) Protein E6 (E6) mRNAs thereby promoting E6 mRNA production. METLL3 induced production of intron-containing HPV16 E1 mRNAs over spliced E2 mRNAs and altered HPV16 L1 mRNA splicing in a manner opposite to ALKBH5. Overexpression of YTHDC1, enhanced retention of the E6-encoding intron and promoted E6 mRNA production. HPV16 mRNAs are m6A-methylated in tonsillar cancer cells.
Responsed Disease Malignant neoplasms of tonsil [ICD-11: 2B69]
Target Regulator RNA demethylase ALKBH5 (ALKBH5) ERASER
 Regulator Info 
Target Regulation Up regulation
In-vitro Model WSU-HN26 Squamous cell carcinoma of the oral cavity Homo sapiens CVCL_5523
HeLa Endocervical adenocarcinoma Homo sapiens CVCL_0030
C33A2 (The C33A2 cell line originates from C33A and has the subgenomic HPV16 plasmid pBELsLuc stably integrated into the genome)
Experiment 2 Reporting the m6A-centered Disease Response by This Target Gene [1]
Response Summary Overexpression of the ALKBH5 promoted production of intron retention on the human papillomavirus type 16 (HPV16) E6 mRNAs thereby promoting Protein E6 (E6) mRNA production. METLL3 induced production of intron-containing HPV16 E1 mRNAs over spliced E2 mRNAs and altered HPV16 L1 mRNA splicing in a manner opposite to ALKBH5. Overexpression of YTHDC1, enhanced retention of the E6-encoding intron and promoted E6 mRNA production. HPV16 mRNAs are m6A-methylated in tonsillar cancer cells.
Responsed Disease Malignant neoplasms of tonsil [ICD-11: 2B69]
Target Regulator YTH domain-containing protein 1 (YTHDC1) READER
 Regulator Info 
Target Regulation Up regulation
In-vitro Model WSU-HN26 Squamous cell carcinoma of the oral cavity Homo sapiens CVCL_5523
HeLa Endocervical adenocarcinoma Homo sapiens CVCL_0030
C33A2 (The C33A2 cell line originates from C33A and has the subgenomic HPV16 plasmid pBELsLuc stably integrated into the genome)
Experiment 3 Reporting the m6A-centered Disease Response by This Target Gene [1]
Response Summary Overexpression of the ALKBH5 promoted production of intron retention on the human papillomavirus type 16 (HPV16) E6 mRNAs thereby promoting Protein E6 (E6) mRNA production.METLL3 induced production of intron-containing HPV16 E1 mRNAs over spliced E2 mRNAs and altered HPV16 L1 mRNA splicing in a manner opposite to ALKBH5. Overexpression of YTHDC1, enhanced retention of the E6-encoding intron and promoted E6 mRNA production. HPV16 mRNAs are m6A-methylated in tonsillar cancer cells.
Responsed Disease Malignant neoplasms of tonsil [ICD-11: 2B69]
Target Regulator Methyltransferase-like 3 (METTL3) WRITER
 Regulator Info 
Target Regulation Up regulation
In-vitro Model WSU-HN26 Squamous cell carcinoma of the oral cavity Homo sapiens CVCL_5523
HeLa Endocervical adenocarcinoma Homo sapiens CVCL_0030
C33A2 (The C33A2 cell line originates from C33A and has the subgenomic HPV16 plasmid pBELsLuc stably integrated into the genome)
References
Ref 1 Overexpression of m6A-factors METTL3, ALKBH5, and YTHDC1 alters HPV16 mRNA splicing. Virus Genes. 2022 Apr;58(2):98-112. doi: 10.1007/s11262-022-01889-6. Epub 2022 Feb 21.