m6A-centered Disease Response Information
General Information of the Disease (ID: M6ADIS0030)
| Name |
Cervical intraepithelial neoplasia
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|---|---|---|---|---|---|
| ICD |
ICD-11: 2E66
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Full List of Target Gene(s) of This m6A-centered Disease Response
Catenin beta-1 (CTNNB1/Beta-catenin)
| In total 1 item(s) under this target gene | ||||
| Experiment 1 Reporting the m6A-centered Disease Response by This Target Gene | [1] | |||
| Response Summary | The mRNA level of FTO is elevated in cervical squamous cell carcinoma (CSCC) tissues when compared with respective adjacent normal tissues.FTO enhances the chemo-radiotherapy resistance both in vitro and in vivo through regulating expression of Catenin beta-1 (CTNNB1/Beta-catenin) by reducing m6A levels in its mRNA transcripts and in turn increases excision repair cross-complementation group 1 (ERCC1) activity. | |||
| Responsed Disease | Cervical squamous cell carcinoma [ICD-11: 2E66.2] | |||
| Target Regulator | Fat mass and obesity-associated protein (FTO) | ERASER | ||
| Target Regulation | Up regulation | |||
| Cell Process | RNA decay | |||
| In-vitro Model | C-33 A | Cervical squamous cell carcinoma | Homo sapiens | CVCL_1094 |
| SiHa | Cervical squamous cell carcinoma | Homo sapiens | CVCL_0032 | |
| In-vivo Model | Either SiHa cells or FTO overexpressed SiHa cells were injected subcutaneously into the right flanks of 4- to 6-week-old female athymic nude mice (CAS, Beijing, China). The mice were divided into four groups (N = 6). After transplantation, tumor size was measured by caliper every other day. Tumor volume was calculated using the formula: volume = (length × width2)/2. When the tumor volumes reached 50 mm3, the animals were treated with intraperitoneal injection of cisplatin (3 mg/kg) every other day for seven times and local irradiation of 8 Gy one time. | |||
DNA excision repair protein ERCC-1 (ERCC1)
| In total 1 item(s) under this target gene | ||||
| Experiment 1 Reporting the m6A-centered Disease Response by This Target Gene | [1] | |||
| Response Summary | The mRNA level of FTO is elevated in cervical squamous cell carcinoma (CSCC) tissues when compared with respective adjacent normal tissues.FTO enhances the chemo-radiotherapy resistance both in vitro and in vivo through regulating expression of beta-catenin by reducing m6A levels in its mRNA transcripts and in turn increases DNA excision repair protein ERCC-1 (ERCC1) activity. | |||
| Responsed Disease | Cervical squamous cell carcinoma [ICD-11: 2E66.2] | |||
| Target Regulator | Fat mass and obesity-associated protein (FTO) | ERASER | ||
| Target Regulation | Up regulation | |||
| Cell Process | RNA decay | |||
| In-vitro Model | C-33 A | Cervical squamous cell carcinoma | Homo sapiens | CVCL_1094 |
| SiHa | Cervical squamous cell carcinoma | Homo sapiens | CVCL_0032 | |
| In-vivo Model | Either SiHa cells or FTO overexpressed SiHa cells were injected subcutaneously into the right flanks of 4- to 6-week-old female athymic nude mice (CAS, Beijing, China). The mice were divided into four groups (N = 6). After transplantation, tumor size was measured by caliper every other day. Tumor volume was calculated using the formula: volume = (length × width2)/2. When the tumor volumes reached 50 mm3, the animals were treated with intraperitoneal injection of cisplatin (3 mg/kg) every other day for seven times and local irradiation of 8 Gy one time. | |||