m6A-centered Crosstalk Information
Mechanism of Crosstalk between m6A Modification and Epigenetic Regulation
| Crosstalk ID |
M6ACROT05783
|
[1] | |||
m6A modification
RNU6-1
RNU6-1
METTL16
Methylation
 : m6A sites
Direct
Enhancement
Non-coding RNA
RNU6-1
Regulated Target
lncRNA miRNA circRNA
|
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| m6A Modification: | |||||
|---|---|---|---|---|---|
| m6A Regulator | Methyltransferase-like 16 (METTL16) | WRITER | |||
| m6A Target | RNA, U6 small nuclear 1 (RNU6-1) | ||||
| Epigenetic Regulation that have Cross-talk with This m6A Modification: | |||||
| Epigenetic Regulation Type | Non-coding RNA (ncRNA) | ||||
| Epigenetic Regulator | RNA, U6 small nuclear 1 (RNU6-1) | snRNA | View Details | ||
| Crosstalk Relationship | m6A → ncRNA | Enhancement | |||
| Crosstalk Mechanism | m6A regulators directly modulate the functionality of ncRNAs through specific targeting ncRNA | ||||
| Crosstalk Summary | Inhibition of METTL16 activity significantly reduces the m6A modification levels of RNA, U6 small nuclear 1 (RNU6-1) | ||||
In-vitro Model |
HEK293 | Normal | Homo sapiens | CVCL_0045 | |
| In-vivo Model | In brief, HEK293 cells expressing the Flag tag or METTL16-Flag were grown in DMEM and crosslinked at 254 nm (UV-CRAC), or alternatively were grown in DMEM supplemented with 100 μM 4-thiouridine for 6 h prior to crosslinking at 365 nm (PAR-CRAC). Samples were then processed and the obtained sequence reads were mapped to the human genome (GRCh37.p13). | ||||