m6A-centered Crosstalk Information

Mechanism of Crosstalk between m6A Modification and Epigenetic Regulation
Crosstalk ID
M6ACROT05706
 [1]
m6A modification Circ_RNF13 Circ_RNF13 METTL3 Methylation : m6A sites Direct Inhibition Non-coding RNA circRNF13 CXCL1  lncRNA       miRNA   circRNA
m6A Modification:
m6A Regulator Methyltransferase-like 3 (METTL3) WRITER
 Regulator Info 
m6A Target Circ_RNF13
 Targert Gene 
Epigenetic Regulation that have Cross-talk with This m6A Modification:
Epigenetic Regulation Type Non-coding RNA (ncRNA)
Epigenetic Regulator Circ_RNF13 circRNA View Details
Regulated Target C-X-C motif chemokine ligand 1 (CXCL1) View Details
Crosstalk Relationship m6A  →  ncRNA Inhibition
Crosstalk Mechanism m6A regulators directly modulate the functionality of ncRNAs through specific targeting ncRNA
Crosstalk Summary METTL3/YTHDF2 promoted the degradation of Circ_RNF13 and subsequently affected the stability of C-X-C motif chemokine ligand 1 (CXCL1), ultimately enhancing the radiosensitivity of CC cells.
Responsed Disease Cervical cancer ICD-11: 2C77
 Disease Info 
Cell Process mRNA stability
In-vitro Model
 SiHa Cervical squamous cell carcinoma Homo sapiens CVCL_0032
C-4-I Cervical squamous cell carcinoma Homo sapiens CVCL_2253
HeLa Endocervical adenocarcinoma Homo sapiens CVCL_0030
C-33 A Cervical squamous cell carcinoma Homo sapiens CVCL_1094
In-vivo Model Stably transfected cell lines were created by silencing circRNF13 in CC SiHa cells. Once xenografts were established, the tumors reached an approximate volume of 200 mm3. A single dose of 15 Gy irradiation was administered to female BALB/c nude mice (4-5 weeks old) in the murine model. The tumor volume was measured and recorded using vernier calipers every five days after irradiation. After 30 days, the mice were euthanized under anesthesia, and tumor tissue was collected for further investigations.
References
Ref 1 circRNF13, a novel N(6)-methyladenosine-modified circular RNA, enhances radioresistance in cervical cancer by increasing CXCL1 mRNA stability. Cell Death Discov. 2023 Jul 20;9(1):253. doi: 10.1038/s41420-023-01557-0.