m6A-centered Crosstalk Information
Mechanism of Crosstalk between m6A Modification and Epigenetic Regulation
| Crosstalk ID |
M6ACROT05687
|
[1] | |||
m6A modification
miR-181a-5p
miR-181a-5p
METTL3
Methylation
 : m6A sites
Direct
Enhancement
Non-coding RNA
miR-181a-5p
NKAP
lncRNA miRNA circRNA
|
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| m6A Modification: | |||||
|---|---|---|---|---|---|
| m6A Regulator | Methyltransferase-like 3 (METTL3) | WRITER | |||
| m6A Target | hsa-miR-181a-5p | ||||
| Epigenetic Regulation that have Cross-talk with This m6A Modification: | |||||
| Epigenetic Regulation Type | Non-coding RNA (ncRNA) | ||||
| Epigenetic Regulator | hsa-miR-181a-5p | microRNA | View Details | ||
| Regulated Target | NFKB activating protein (NKAP) | View Details | |||
| Crosstalk Relationship | m6A → ncRNA | Enhancement | |||
| Crosstalk Mechanism | m6A regulators directly modulate the functionality of ncRNAs through specific targeting ncRNA | ||||
| Crosstalk Summary | METTL3 was able to promote the maturation of hsa-miR-181a-5p that targets NFKB activating protein (NKAP), and then inhibited the expression of NF-kappaB and IL-1alpha. | ||||
| Responsed Disease | Ageing-related disease | ICD-11: 9B10-9B60 | |||
| Cell Process | Cell senescence | ||||
In-vitro Model |
HK-2 [Human kidney] | Normal | Homo sapiens | CVCL_0302 | |
| In-vivo Model | The mice were randomly separated into four groups (n = 3-5): young mice group (YNG); naturally aging mice group (OLD); PBS-treated mice group (Con); D-gal-treated aging mice group (D-gal). YNG group was the directly purchased mice (8-week-old) actually. OLD group: mice bought continued to be raised in a specific pathogen-free facility (SPF) individually for 24 months. Mice in Con group were injected subcutaneously with phosphate buffered saline (PBS) solution formulated from powder, with the same volume as the D-gal group. Mice from D-gal group were made by injecting (D-gal, 500 mg/kg body weight) daily for 8 weeks, subcutaneously. All animals were kept under a standard environmental condition (25 ± 2 °C; 12-h light-dark cycle) and allowed free access to water and regular sterile chow diets. All animal studies were designed in accordance with ARRIVE guidelines and experiment protocols followed NIH guidelines. | ||||