m6A-centered Crosstalk Information
Mechanism of Crosstalk between m6A Modification and Epigenetic Regulation
| Crosstalk ID |
M6ACROT05448
|
[1] | |||
m6A modification
KCNMB2-AS1
KCNMB2-AS1
IGF2BP3
 : m6A sites
Direct
Enhancement
Non-coding RNA
KCNMB2-AS1
miR-130b-5p
lncRNA miRNA circRNA
|
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| m6A Modification: | |||||
|---|---|---|---|---|---|
| m6A Regulator | Insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3) | READER | |||
| m6A Target | KCNMB2 antisense RNA 1 (KCNMB2-AS1) | ||||
| Epigenetic Regulation that have Cross-talk with This m6A Modification: | |||||
| Epigenetic Regulation Type | Non-coding RNA (ncRNA) | ||||
| Epigenetic Regulator | KCNMB2 antisense RNA 1 (KCNMB2-AS1) | LncRNA | View Details | ||
| Regulated Target | hsa-miR-130b-5p | View Details | |||
| Crosstalk Relationship | m6A → ncRNA | Enhancement | |||
| Crosstalk Mechanism | m6A regulators directly modulate the functionality of ncRNAs through specific targeting ncRNA | ||||
| Crosstalk Summary | KCNMB2 antisense RNA 1 (KCNMB2-AS1) and IGF2BP3 formed a positive regulatory circuit that enlarged the tumorigenic effect of KCNMB2-AS1 in cervical cancer. KCNMB2-AS1 was predominantly located in the cytoplasm and served as a competing endogenous RNA to abundantly sponge hsa-miR-130b-5p and miR-4294, resulting in the upregulation of IGF2BP3, a well-documented oncogene in CC. | ||||
| Responsed Disease | Cervical cancer | ICD-11: 2C77 | |||
| Pathway Response | Apoptosis | hsa04210 | |||
| Cell Process | Cell proliferation | ||||
| Cell apoptosis | |||||
In-vitro Model |
SiHa | Cervical squamous cell carcinoma | Homo sapiens | CVCL_0032 | |
| HeLa | Endocervical adenocarcinoma | Homo sapiens | CVCL_0030 | ||
| In-vivo Model | A total of 1 × 107 control or KCNMB2-AS1-depleted SiHa cells were resuspended in 0.1 ml phosphate-buffered saline and inoculated into the armpit of 5-week-old male BALB/c nude mice. | ||||