m6A-centered Crosstalk Information
Mechanism of Crosstalk between m6A Modification and Epigenetic Regulation
| Crosstalk ID |
M6ACROT05441
|
[1] | |||
m6A modification
THORLNC
THORLNC
METTL3
Methylation
 : m6A sites
Direct
Enhancement
Non-coding RNA
THOR
Regulated Target
lncRNA miRNA circRNA
|
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| m6A Modification: | |||||
|---|---|---|---|---|---|
| m6A Regulator | Methyltransferase-like 3 (METTL3) | WRITER | |||
| m6A Target | Testis associated oncogenic lncRNA (THORLNC) | ||||
| Epigenetic Regulation that have Cross-talk with This m6A Modification: | |||||
| Epigenetic Regulation Type | Non-coding RNA (ncRNA) | ||||
| Epigenetic Regulator | Testis associated oncogenic lncRNA (THORLNC) | LncRNA | View Details | ||
| Crosstalk Relationship | m6A → ncRNA | Enhancement | |||
| Crosstalk Mechanism | m6A regulators directly modulate the functionality of ncRNAs through specific targeting ncRNA | ||||
| Crosstalk Summary | The significantly reduced gene expression of the lncRNA Testis associated oncogenic lncRNA (THORLNC) and the decreased m6A level on the lncRNA THOR in the METTL3stop/stop cells were determined by qRT-PCR and methylated RNA immunoprecipitation (MeRIP) assay. RIP-qRT-PCR and RNA pull-down assay results revealed that the specific m6A readers YTHDF1 and YTHDF2 can read the m6A motifs and regulate the stability of the lncRNA THOR (stabilization and decay). | ||||
| Cell Process | Cell proliferation | ||||
| Cell migration | |||||
| Cell invasion | |||||
In-vitro Model |
NCI-H1299 | Lung large cell carcinoma | Homo sapiens | CVCL_0060 | |
| In-vivo Model | 2 × 106 cells were suspended in 200 uL of serum-free RPMI 1640 for injection into each mouse. | ||||