m6A-centered Crosstalk Information
Mechanism of Crosstalk between m6A Modification and Epigenetic Regulation
| Crosstalk ID |
M6ACROT05014
|
[1], [2] | |||
Non-coding RNA
DARS-AS1
IGF2BP3
lncRNA miRNA circRNA
Direct
Enhancement
m6A modification
MARCKSL1
MARCKSL1
IGF2BP3
 : m6A sites
|
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| m6A Modification: | |||||
|---|---|---|---|---|---|
| m6A Regulator | Insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3) | READER | |||
| m6A Target | MARCKS-related protein (MARCKSL1) | ||||
| Epigenetic Regulation that have Cross-talk with This m6A Modification: | |||||
| Epigenetic Regulation Type | Non-coding RNA (ncRNA) | ||||
| Epigenetic Regulator | DARS1 antisense RNA 1 (DARS1-AS1) | LncRNA | View Details | ||
| Regulated Target | Insulin like growth factor 2 mRNA binding protein 3 (IGF2BP3) | View Details | |||
| Crosstalk Relationship | ncRNA → m6A | Enhancement | |||
| Crosstalk Mechanism | ncRNAs directly impacts m6A modification through modulating the expression level of m6A regulator | ||||
| Crosstalk Summary | Downregulation of LncRNA DARS1-AS1 Inhibits the Tumorigenesis of Cervical Cancer via Inhibition of IGF2BP3, In contrast to the mRNA-decay-promoting function of YTH domain-containing family protein 2, IGF2BPs promote the stability and storage of their target mRNAs (for example, MYC) in an m6A-dependent manner under normal and stress conditions and therefore affect gene expression output. Four representative high confidence targets, including MYC, FSCN1, TK1, and MARCKS-related protein (MARCKSL1), exhibit strong binding with IGF2BPs around their m6A motifs in control cells. Knocking down of each individual IGF2BPs in Hela (cervical cancer) and HepG2 (liver cancer) cells significantly repressed MYC expression. | ||||
| Responsed Disease | Cervical cancer | ICD-11: 2C77 | |||
| Cell Process | RNA decay | ||||
In-vitro Model |
SiHa | Cervical squamous cell carcinoma | Homo sapiens | CVCL_0032 | |
| HeLa | Endocervical adenocarcinoma | Homo sapiens | CVCL_0030 | ||
| Hep-G2 | Hepatoblastoma | Homo sapiens | CVCL_0027 | ||
| HEK293T | Normal | Homo sapiens | CVCL_0063 | ||
| HeLa | Endocervical adenocarcinoma | Homo sapiens | CVCL_0030 | ||
References