m6A-centered Crosstalk Information

Mechanism of Crosstalk between m6A Modification and Epigenetic Regulation

m6A Modification:
Crosstalk ID	M6ACROT00606				[1], [2], [3]
m⁶A modification MIR320A MIR320A METTL3 Methylation : m⁶A sites Indirect Inhibition RNA modification NANOG NANOG YBX1 : modification sites
m6A Regulator	Methyltransferase-like 3 (METTL3)			WRITER	Regulator Info
m6A Target	hsa-mir-320a				Targert Gene
Epigenetic Regulation that have Cross-talk with This m6A Modification:
Epigenetic Regulation Type	RNA modification (RNAMod) >> 5-methylcytidine (m5C)
Epigenetic Regulator	Y-box-binding protein 1 (YBX1)			READER	View Details
Regulated Target	Homeobox protein NANOG (NANOG)				View Details
Crosstalk Relationship	m6A → m5C			Inhibition
Crosstalk Mechanism	m6A modification indirectly impacts RNA modification through downstream signaling pathways
Crosstalk Summary	METTL3 interacts with hsa-mir-320a, reading its m6A level and inhibiting its physical interaction with Homeobox protein NANOG (NANOG), which was regulated by YBX1-mediated m5C modification.
In-vitro Model	NCI-H1299	Lung large cell carcinoma	Homo sapiens		CVCL_0060

References

Ref 1	Transcriptional activation of NANOG by YBX1 promotes lung cancer stem-like properties and metastasis. Biochem Biophys Res Commun. 2017 May 20;487(1):153-159. doi: 10.1016/j.bbrc.2017.04.033. Epub 2017 Apr 8.
Ref 2	m(6)A Methylation of Precursor-miR-320/RUNX2 Controls Osteogenic Potential of Bone Marrow-Derived Mesenchymal Stem Cells. Mol Ther Nucleic Acids. 2020 Mar 6;19:421-436. doi: 10.1016/j.omtn.2019.12.001. Epub 2019 Dec 12.
Ref 3	MiR-320a was highly expressed in postmenopausal osteoporosis and acts as a negative regulator in MC3T3E1 cells by reducing MAP9 and inhibiting PI3K/AKT signaling pathway. Exp Mol Pathol. 2019 Oct;110:104282. doi: 10.1016/j.yexmp.2019.104282. Epub 2019 Jul 10.