m6A-centered Crosstalk Information

Mechanism of Crosstalk between m6A Modification and Epigenetic Regulation

m6A Modification:
Crosstalk ID	M6ACROT00602				[1], [2], [3], [4], [5], [6]
m⁶A modification MIR21 MIR21 METTL3 Methylation : m⁶A sites Indirect Enhancement RNA modification PTEN PTEN TET3 Demethylation : modification sites
m6A Regulator	Methyltransferase-like 3 (METTL3)			WRITER	Regulator Info
m6A Target	microRNA 21 (MIR21)				Targert Gene
Epigenetic Regulation that have Cross-talk with This m6A Modification:
Epigenetic Regulation Type	RNA modification (RNAMod) >> 5-methylcytidine (m5C)
Epigenetic Regulator	Methylcytosine dioxygenase TET3 (TET3)			ERASER	View Details
Regulated Target	Mutated in multiple advanced cancers 1 (PTEN)				View Details
Crosstalk Relationship	m6A → m5C			Enhancement
Crosstalk Mechanism	m6A modification indirectly impacts RNA modification through downstream signaling pathways
Crosstalk Summary	METTL3 interacts with microRNA 21 (MIR21), decreasing its m6A level and promoting its physical interaction with Mutated in multiple advanced cancers 1 (PTEN), which was regulated by TET3-mediated m5C modification.
In-vitro Model	Hep 3B2.1-7	Childhood hepatocellular carcinoma	Homo sapiens		CVCL_0326
	Hep-G2	Hepatoblastoma	Homo sapiens		CVCL_0027
	HK2	Normal	Acipenser baerii		CVCL_YE28

References

Ref 1	Exosomal miR-21 regulates the TETs/PTENp1/PTEN pathway to promote hepatocellular carcinoma growth. Mol Cancer. 2019 Oct 27;18(1):148. doi: 10.1186/s12943-019-1075-2.
Ref 2	METTL3/N6-methyladenosine/ miR-21-5p promotes obstructive renal fibrosis by regulating inflammation through SPRY1/ERK/NF-KappaB pathway activation. J Cell Mol Med. 2021 Aug;25(16):7660-7674. doi: 10.1111/jcmm.16603. Epub 2021 Jun 24.
Ref 3	Long non-coding RNA MEG3 promotes autophagy and apoptosis of nasopharyngeal carcinoma cells via PTEN up-regulation by binding to microRNA-21. J Cell Mol Med. 2021 Jan;25(1):61-72. doi: 10.1111/jcmm.15759. Epub 2020 Dec 17.
Ref 4	Long non?coding RNA MEG3 inhibits cell migration and invasion of non?small cell lung cancer cells by regulating the miR?21?5p/PTEN axis. Mol Med Rep. 2021 Mar;23(3):191. doi: 10.3892/mmr.2021.11830. Epub 2021 Jan 26.
Ref 5	lncRNA TP73-AS1 Regulates miR-21/PTEN Axis to Affect Cell Proliferation in Acute Myeloid Leukemia. Cancer Biother Radiopharm. 2021 Apr;36(3):268-272. doi: 10.1089/cbr.2019.3142. Epub 2020 May 15.
Ref 6	The pseudogene PTENP1 regulates smooth muscle cells as a competing endogenous RNA. Clin Sci (Lond). 2019 Jul 15;133(13):1439-1455. doi: 10.1042/CS20190156. Print 2019 Jul 15.