m6A-centered Crosstalk Information

Mechanism of Crosstalk between m6A Modification and Epigenetic Regulation

m6A Modification:
Crosstalk ID	M6ACROT00594				[1], [2], [3], [4], [5], [6]
RNA modification GAS5 GAS5 YBX1 : modification sites Indirect Enhancement m⁶A modification MIR21 MIR21 METTL3 Methylation : m⁶A sites
m6A Regulator	Methyltransferase-like 3 (METTL3)			WRITER	Regulator Info
m6A Target	microRNA 21 (MIR21)				Targert Gene
Epigenetic Regulation that have Cross-talk with This m6A Modification:
Epigenetic Regulation Type	RNA modification (RNAMod) >> 5-methylcytidine (m5C)
Epigenetic Regulator	Y-box-binding protein 1 (YBX1)			READER	View Details
Regulated Target	Growth arrest specific 5 (GAS5)				View Details
Crosstalk Relationship	m5C → m6A			Enhancement
Crosstalk Mechanism	RNA modification indirectly impacts m6A modification through downstream signaling pathways
Crosstalk Summary	YBX1 interacts with Growth arrest specific 5 (GAS5), reading its m5C sites and promoting its physical interaction with microRNA 21 (MIR21), which was regulated by METTL3-mediated m6A modification.
In-vitro Model	HGC-27	Gastric carcinoma	Homo sapiens		CVCL_1279
In-vitro Model	HK2	Normal	Acipenser baerii		CVCL_YE28

References

Ref 1	lncRNA GAS5 enhances G1 cell cycle arrest via binding to YBX1 to regulate p21 expression in stomach cancer. Sci Rep. 2015 May 11;5:10159. doi: 10.1038/srep10159.
Ref 2	Long noncoding RNA GAS5 induces abdominal aortic aneurysm formation by promoting smooth muscle apoptosis. Theranostics. 2019 Jul 28;9(19):5558-5576. doi: 10.7150/thno.34463. eCollection 2019.
Ref 3	METTL3/N6-methyladenosine/ miR-21-5p promotes obstructive renal fibrosis by regulating inflammation through SPRY1/ERK/NF-KappaB pathway activation. J Cell Mol Med. 2021 Aug;25(16):7660-7674. doi: 10.1111/jcmm.16603. Epub 2021 Jun 24.
Ref 4	lncRNA GAS5 acts as a ceRNA for miR-21 in suppressing PDGF-bb-induced proliferation and migration in vascular smooth muscle cells. J Cell Biochem. 2019 Sep;120(9):15233-15240. doi: 10.1002/jcb.28789. Epub 2019 May 8.
Ref 5	Long Noncoding RNA GAS5 Acts As A Tumor Suppressor In Laryngeal Squamous Cell Carcinoma Via miR-21. Cancer Manag Res. 2019 Sep 17;11:8487-8498. doi: 10.2147/CMAR.S213690. eCollection 2019.
Ref 6	GAS5/miR-21 Axis as a Potential Target to Rescue ZCL-082-Induced Autophagy of Female Germline Stem Cells In?Vitro. Mol Ther Nucleic Acids. 2019 Sep 6;17:436-447. doi: 10.1016/j.omtn.2019.06.012. Epub 2019 Jun 28.