m6A-centered Crosstalk Information

Mechanism of Crosstalk between m6A Modification and Epigenetic Regulation

m6A Modification:
Crosstalk ID	M6ACROT00501				[1], [2], [3]
RNA modification MIR222 MIR222 ADARB1 Methylation : modification sites Indirect Inhibition m⁶A modification CDKN1B CDKN1B WTAP Methylation : m⁶A sites
m6A Regulator	Wilms tumor 1-associating protein (WTAP)			WRITER	Regulator Info
m6A Target	Cyclin-dependent kinase inhibitor 1B (CDKN1B/p27)				Targert Gene
Epigenetic Regulation that have Cross-talk with This m6A Modification:
Epigenetic Regulation Type	RNA modification (RNAMod) >> Adenosine-to-Inosine editing (A-to-I)
Epigenetic Regulator	Double-stranded RNA-specific editase 1 (ADARB1)			WRITER	View Details
Regulated Target	MicroRNA 222 (MIR222)				View Details
Crosstalk Relationship	A-to-I → m6A			Inhibition
Crosstalk Mechanism	RNA modification indirectly impacts m6A modification through downstream signaling pathways
Crosstalk Summary	ADARB1 interacts with MicroRNA 222 (MIR222), increasing it's A-to-I level and inhibiting its physical interaction with Cyclin-dependent kinase inhibitor 1B (CDKN1B/p27), which was regulated by WTAP-mediated m6A modification.
In-vitro Model	Huh-7	Adult hepatocellular carcinoma	Homo sapiens		CVCL_0336
	Hep 3B2.1-7	Childhood hepatocellular carcinoma	Homo sapiens		CVCL_0326
	HCCLM3	Adult hepatocellular carcinoma	Homo sapiens		CVCL_6832
	U-118MG	Astrocytoma	Homo sapiens		CVCL_0633

References

Ref 1	WTAP facilitates progression of hepatocellular carcinoma via m6A-HuR-dependent epigenetic silencing of ETS1. Mol Cancer. 2019 Aug 22;18(1):127. doi: 10.1186/s12943-019-1053-8.
Ref 2	Modulation of microRNA editing, expression and processing by ADAR2 deaminase in glioblastoma. Genome Biol. 2015 Jan 13;16(1):5. doi: 10.1186/s13059-014-0575-z.
Ref 3	MiR-222 promotes the progression of polycystic ovary syndrome by targeting p27 Kip1. Pathol Res Pract. 2019 May;215(5):918-923. doi: 10.1016/j.prp.2019.01.038. Epub 2019 Jan 28.