m6A-centered Crosstalk Information

Mechanism of Crosstalk between m6A Modification and Epigenetic Regulation

m6A Modification:
Crosstalk ID	M6ACROT00447				[1], [2], [3], [4], [5], [6]
RNA modification MIR214 MIR214 ADARB1 Methylation : modification sites Indirect Inhibition m⁶A modification PTEN PTEN METTL3 Methylation : m⁶A sites
m6A Regulator	Methyltransferase-like 3 (METTL3)			WRITER	Regulator Info
m6A Target	Mutated in multiple advanced cancers 1 (PTEN)				Targert Gene
Epigenetic Regulation that have Cross-talk with This m6A Modification:
Epigenetic Regulation Type	RNA modification (RNAMod) >> Adenosine-to-Inosine editing (A-to-I)
Epigenetic Regulator	Double-stranded RNA-specific editase 1 (ADARB1)			WRITER	View Details
Regulated Target	MicroRNA 214 (MIR214)				View Details
Crosstalk Relationship	A-to-I → m6A			Inhibition
Crosstalk Mechanism	RNA modification indirectly impacts m6A modification through downstream signaling pathways
Crosstalk Summary	ADARB1 interacts with MicroRNA 214 (MIR214), increasing it's A-to-I level and inhibiting its physical interaction with Mutated in multiple advanced cancers 1 (PTEN), which was regulated by METTL3-mediated m6A modification.
In-vitro Model	MOLM-13	Adult acute myeloid leukemia	Homo sapiens		CVCL_2119
	ARPE-19	Normal	Homo sapiens		CVCL_0145
	K-562	Chronic myelogenous leukemia	Homo sapiens		CVCL_0004
	Huh-7	Adult hepatocellular carcinoma	Homo sapiens		CVCL_0336

References

Ref 1	The N(6)-methyladenosine (m(6)A)-forming enzyme METTL3 controls myeloid differentiation of normal hematopoietic and leukemia cells. Nat Med. 2017 Nov;23(11):1369-1376. doi: 10.1038/nm.4416. Epub 2017 Sep 18.
Ref 2	Overexpression of METTL3 attenuates high-glucose induced RPE cell pyroptosis by regulating miR-25-3p/PTEN/Akt signaling cascade through DGCR8. Aging (Albany NY). 2020 May 4;12(9):8137-8150. doi: 10.18632/aging.103130. Epub 2020 May 4.
Ref 3	Dysregulation of LINC00470 and METTL3 promotes chemoresistance and suppresses autophagy of chronic myelocytic leukaemia cells. J Cell Mol Med. 2021 May;25(9):4248-4259. doi: 10.1111/jcmm.16478. Epub 2021 Mar 21.
Ref 4	ADAR2-mediated editing of miR-214 and miR-122 precursor and antisense RNA transcripts in liver cancers. PLoS One. 2013 Dec 27;8(12):e81922. doi: 10.1371/journal.pone.0081922. eCollection 2013.
Ref 5	Neuroprotection of miR-214 against isoflurane-induced neurotoxicity involves the PTEN/PI3K/Akt pathway in human neuroblastoma cell line SH-SY5Y. Arch Biochem Biophys. 2019 Dec 15;678:108181. doi: 10.1016/j.abb.2019.108181. Epub 2019 Nov 5.
Ref 6	LncRNA TUBA4B functions as a competitive endogenous RNA to inhibit gastric cancer progression by elevating PTEN via sponging miR-214 and miR-216a/b. Cancer Cell Int. 2019 Jun 7;19:156. doi: 10.1186/s12935-019-0879-x. eCollection 2019.