m6A Target Gene Information

General Information of the m6A Target Gene (ID: M6ATAR00727)
Target Name Gap junction alpha-1 protein (GJA1)
Synonyms
Connexin-43; Cx43; Gap junction 43 kDa heart protein
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Gene Name GJA1
Chromosomal Location 6q22.31
Family Connexin family, Alpha-type (group II) subfamily
Function
Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract (By similarity). May play a role in cell growth inhibition through the regulation of NOV expression and localization. Plays an essential role in gap junction communication in the ventricles (By similarity).
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Gene ID 2697
Uniprot ID
CXA1_HUMAN
HGNC ID
HGNC:4274
Ensembl Gene ID
ENSG00000152661
KEGG ID
hsa:2697
Full List of m6A Methylation Regulator of This Target Gene and Corresponding Disease/Drug Response(s)
GJA1 can be regulated by the following regulator(s), and cause disease/drug response(s). You can browse detail information of regulator(s) or disease/drug response(s).
Browse Regulator
Browse Disease
YTH domain-containing family protein 3 (YTHDF3) [READER]
 Regulator Info 
Representative RNA-seq result indicating the expression of this target gene regulated by YTHDF3
Cell Line Mouse embryonic fibroblasts Mus musculus
Treatment: shYthdf3 embryonic fibroblasts
Control: shLuc embryonic fibroblasts
 GSE156437
Regulation
logFC: -7.79E-01
p-value: 7.79E-03
More Results Click to View More RNA-seq Results
In total 1 item(s) under this regulator
Experiment 1 Reporting the m6A Methylation Regulator of This Target Gene [1]
Response Summary Mechanistically, YTHDF3 enhances the translation of m6A-enriched transcripts for ST6GALNAC5, Gap junction alpha-1 protein (GJA1), and EGFR, all associated with breast cancer brain metastasis. This work uncovers an essential role of YTHDF3 in controlling the interaction between cancer cells and brain microenvironment, thereby inducing brain metastatic competence.
Target Regulation Up regulation
Responsed Disease Breast cancer ICD-11: 2C60
 Disease Info 
Cell Process Cell metastasis
In-vitro Model MDA-MB-231Br (After brain metastases of MDA-MB-361 breast adenocarcinoma cells)
MDA-MB-231 Breast adenocarcinoma Homo sapiens CVCL_0062
MDA-IBC-3 Breast inflammatory carcinoma Homo sapiens CVCL_HC47
JIMT-1Br3 (After brain metastases of JIMT-1 breast cancer cells)
JIMT-1 Breast ductal carcinoma Homo sapiens CVCL_2077
HEK293-FT Normal Homo sapiens CVCL_6911
HCC1954Br (After brain metastases of HCC1954 breast cancer cells)
HCC1954 Breast ductal carcinoma Homo sapiens CVCL_1259
bEnd.3 Cerebrovascular endothelioma cells from mice Mus musculus CVCL_0170
BEAS-2B Normal Homo sapiens CVCL_0168
4T1Br (After brain metastases of 4T1 mouse breast cancer cells)
4T1 Normal Mus musculus CVCL_0125
In-vivo Model For the in vivo brain and bone extravasation and seeding assays, cancer cells labeled with CMFDA C2925 (Thermo fisher scientific) or GFP were injected intracardially into the nude mice. Cell number and injection procedure were described in "Animal Experiments". For the in vivo lung extravasation and seeding assays, cancer cells labeled with GFP (2.5 × 105 cells/mouse) were injected into the tail vein of nude mice. At 24 or 48 hrs later, the mice were sacrificed.
Breast cancer [ICD-11: 2C60]
 Disease Info 
In total 1 item(s) under this disease
Experiment 1 Reporting the m6A-centered Disease Response [1]
Response Summary Mechanistically, YTHDF3 enhances the translation of m6A-enriched transcripts for ST6GALNAC5, Gap junction alpha-1 protein (GJA1), and EGFR, all associated with breast cancer brain metastasis. This work uncovers an essential role of YTHDF3 in controlling the interaction between cancer cells and brain microenvironment, thereby inducing brain metastatic competence.
Responsed Disease Breast cancer [ICD-11: 2C60]
Target Regulator YTH domain-containing family protein 3 (YTHDF3) READER
 Regulator Info 
Target Regulation Up regulation
Cell Process Cell metastasis
In-vitro Model MDA-MB-231Br (After brain metastases of MDA-MB-361 breast adenocarcinoma cells)
MDA-MB-231 Breast adenocarcinoma Homo sapiens CVCL_0062
MDA-IBC-3 Breast inflammatory carcinoma Homo sapiens CVCL_HC47
JIMT-1Br3 (After brain metastases of JIMT-1 breast cancer cells)
JIMT-1 Breast ductal carcinoma Homo sapiens CVCL_2077
HEK293-FT Normal Homo sapiens CVCL_6911
HCC1954Br (After brain metastases of HCC1954 breast cancer cells)
HCC1954 Breast ductal carcinoma Homo sapiens CVCL_1259
bEnd.3 Cerebrovascular endothelioma cells from mice Mus musculus CVCL_0170
BEAS-2B Normal Homo sapiens CVCL_0168
4T1Br (After brain metastases of 4T1 mouse breast cancer cells)
4T1 Normal Mus musculus CVCL_0125
In-vivo Model For the in vivo brain and bone extravasation and seeding assays, cancer cells labeled with CMFDA C2925 (Thermo fisher scientific) or GFP were injected intracardially into the nude mice. Cell number and injection procedure were described in "Animal Experiments". For the in vivo lung extravasation and seeding assays, cancer cells labeled with GFP (2.5 × 105 cells/mouse) were injected into the tail vein of nude mice. At 24 or 48 hrs later, the mice were sacrificed.
References
Ref 1 YTHDF3 Induces the Translation of m(6)A-Enriched Gene Transcripts to Promote Breast Cancer Brain Metastasis. Cancer Cell. 2020 Dec 14;38(6):857-871.e7. doi: 10.1016/j.ccell.2020.10.004. Epub 2020 Oct 29.