m6A Target Gene Information

General Information of the m6A Target Gene (ID: M6ATAR00726)
Target Name GD1 alpha synthase (ST6GALNAC5)
Synonyms
GD1 alpha synthase; GalNAc alpha-2,6-sialyltransferase V; ST6GalNAc V; ST6GalNAcV; Sialyltransferase 7E; SIAT7-E
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Gene Name ST6GALNAC5
Chromosomal Location 1p31.1
Family Glycosyltransferase 29 family
Function
Predominantly catalyzes the biosynthesis of ganglioside GD1alpha from GM1b in the brain, by transferring the sialyl group (N-acetyl-alpha-neuraminyl or NeuAc) from CMP-NeuAc to the GalNAc residue on the NeuAc-alpha-2,3-Gal-beta-1,3-GalNAc sequence of GM1b. GD1alpha is a critical molecule in the communication and interaction between neuronal cells and their supportive cells, particularly in brain tissues, and functions as an adhesion molecule in the process of metastasis (By similarity). Also shows activity towards sialyl Lc4Cer (N-acetyl-alpha-neuraminosyl-(2->3)-beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1')-N-acyl-sphing-4-enine) generating disialyl Lc4Cer, which can lead to the synthesis of disialyl Lewis a (Le(a)), suggested to be a cancer-associated antige .
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Gene ID 81849
Uniprot ID
SIA7E_HUMAN
HGNC ID
HGNC:19342
Ensembl Gene ID
ENSG00000117069
KEGG ID
hsa:81849
Full List of m6A Methylation Regulator of This Target Gene and Corresponding Disease/Drug Response(s)
ST6GALNAC5 can be regulated by the following regulator(s), and cause disease/drug response(s). You can browse detail information of regulator(s) or disease/drug response(s).
Browse Regulator
Browse Disease
YTH domain-containing family protein 3 (YTHDF3) [READER]
 Regulator Info 
In total 1 item(s) under this regulator
Experiment 1 Reporting the m6A Methylation Regulator of This Target Gene [1]
Response Summary Mechanistically, YTHDF3 enhances the translation of m6A-enriched transcripts for GD1 alpha synthase (ST6GALNAC5), GJA1, and EGFR, all associated with breast cancer brain metastasis. This work uncovers an essential role of YTHDF3 in controlling the interaction between cancer cells and brain microenvironment, thereby inducing brain metastatic competence.
Target Regulation Up regulation
Responsed Disease Breast cancer ICD-11: 2C60
 Disease Info 
Cell Process Cell metastasis
In-vitro Model MDA-MB-231Br (After brain metastases of MDA-MB-361 breast adenocarcinoma cells)
MDA-MB-231 Breast adenocarcinoma Homo sapiens CVCL_0062
MDA-IBC-3 Breast inflammatory carcinoma Homo sapiens CVCL_HC47
JIMT-1Br3 (After brain metastases of JIMT-1 breast cancer cells)
JIMT-1 Breast ductal carcinoma Homo sapiens CVCL_2077
HEK293-FT Normal Homo sapiens CVCL_6911
HCC1954Br (After brain metastases of HCC1954 breast cancer cells)
HCC1954 Breast ductal carcinoma Homo sapiens CVCL_1259
bEnd.3 Cerebrovascular endothelioma cells from mice Mus musculus CVCL_0170
BEAS-2B Normal Homo sapiens CVCL_0168
4T1Br (After brain metastases of 4T1 mouse breast cancer cells)
4T1 Normal Mus musculus CVCL_0125
In-vivo Model For the in vivo brain and bone extravasation and seeding assays, cancer cells labeled with CMFDA C2925 (Thermo fisher scientific) or GFP were injected intracardially into the nude mice. Cell number and injection procedure were described in "Animal Experiments". For the in vivo lung extravasation and seeding assays, cancer cells labeled with GFP (2.5 × 105 cells/mouse) were injected into the tail vein of nude mice. At 24 or 48 hrs later, the mice were sacrificed.
Breast cancer [ICD-11: 2C60]
 Disease Info 
In total 1 item(s) under this disease
Experiment 1 Reporting the m6A-centered Disease Response [1]
Response Summary Mechanistically, YTHDF3 enhances the translation of m6A-enriched transcripts for GD1 alpha synthase (ST6GALNAC5), GJA1, and EGFR, all associated with breast cancer brain metastasis. This work uncovers an essential role of YTHDF3 in controlling the interaction between cancer cells and brain microenvironment, thereby inducing brain metastatic competence.
Responsed Disease Breast cancer [ICD-11: 2C60]
Target Regulator YTH domain-containing family protein 3 (YTHDF3) READER
 Regulator Info 
Target Regulation Up regulation
Cell Process Cell metastasis
In-vitro Model MDA-MB-231Br (After brain metastases of MDA-MB-361 breast adenocarcinoma cells)
MDA-MB-231 Breast adenocarcinoma Homo sapiens CVCL_0062
MDA-IBC-3 Breast inflammatory carcinoma Homo sapiens CVCL_HC47
JIMT-1Br3 (After brain metastases of JIMT-1 breast cancer cells)
JIMT-1 Breast ductal carcinoma Homo sapiens CVCL_2077
HEK293-FT Normal Homo sapiens CVCL_6911
HCC1954Br (After brain metastases of HCC1954 breast cancer cells)
HCC1954 Breast ductal carcinoma Homo sapiens CVCL_1259
bEnd.3 Cerebrovascular endothelioma cells from mice Mus musculus CVCL_0170
BEAS-2B Normal Homo sapiens CVCL_0168
4T1Br (After brain metastases of 4T1 mouse breast cancer cells)
4T1 Normal Mus musculus CVCL_0125
In-vivo Model For the in vivo brain and bone extravasation and seeding assays, cancer cells labeled with CMFDA C2925 (Thermo fisher scientific) or GFP were injected intracardially into the nude mice. Cell number and injection procedure were described in "Animal Experiments". For the in vivo lung extravasation and seeding assays, cancer cells labeled with GFP (2.5 × 105 cells/mouse) were injected into the tail vein of nude mice. At 24 or 48 hrs later, the mice were sacrificed.
References
Ref 1 YTHDF3 Induces the Translation of m(6)A-Enriched Gene Transcripts to Promote Breast Cancer Brain Metastasis. Cancer Cell. 2020 Dec 14;38(6):857-871.e7. doi: 10.1016/j.ccell.2020.10.004. Epub 2020 Oct 29.