m6A Target Gene Information

General Information of the m6A Target Gene (ID: M6ATAR00520)
Target Name Alpha-enolase (ENO1)
Synonyms
2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding protein; Enolase 1; MBP-1; MPB-1; Non-neural enolase; NNE; Phosphopyruvate hydratase; Plasminogen-binding protein
    Click to Show/Hide
Gene Name ENO1
Chromosomal Location 1p36.23
Family Enolase family
Function
Glycolytic enzyme the catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. In addition to glycolysis, involved in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
    Click to Show/Hide
Gene ID 2023
Uniprot ID
ENOA_HUMAN
HGNC ID
HGNC:3350
Ensembl Gene ID
ENSG00000074800
KEGG ID
hsa:2023
Full List of m6A Methylation Regulator of This Target Gene and Corresponding Disease/Drug Response(s)
ENO1 can be regulated by the following regulator(s), and cause disease/drug response(s). You can browse detail information of regulator(s) or disease/drug response(s).
Browse Regulator
Browse Disease
Methyltransferase-like 3 (METTL3) [WRITER]
 Regulator Info 
Representative RNA-seq result indicating the expression of this target gene regulated by METTL3
Cell Line mouse embryonic stem cells Mus musculus
Treatment: METTL3-/- ESCs
Control: Wild type ESCs
 GSE145309
Regulation
logFC: -8.80E-01
p-value: 3.03E-81
More Results Click to View More RNA-seq Results
In total 1 item(s) under this regulator
Experiment 1 Reporting the m6A Methylation Regulator of This Target Gene [1]
Response Summary Alpha-enolase (ENO1) positively correlated with METTL3 and global m6A levels, and negatively correlated with ALKBH5 in human Lung adenocarcinoma(LUAD). In addition, m6A-dependent elevation of ENO1 was associated with LUAD progression.
Target Regulation Up regulation
Responsed Disease Lung adenocarcinoma ICD-11: 2C25.0
 Disease Info 
Pathway Response Carbon metabolism hsa01200
Glycolysis / Gluconeogenesis hsa00010
Cell Process Glycolysis
In-vitro Model PC-9 Lung adenocarcinoma Homo sapiens CVCL_B260
NCI-H441 Lung papillary adenocarcinoma Homo sapiens CVCL_1561
NCI-H292 Lung mucoepidermoid carcinoma Homo sapiens CVCL_0455
NCI-H2030 Lung adenocarcinoma Homo sapiens CVCL_1517
NCI-H1975 Lung adenocarcinoma Homo sapiens CVCL_1511
NCI-H1650 Minimally invasive lung adenocarcinoma Homo sapiens CVCL_1483
NCI-H1299 Lung large cell carcinoma Homo sapiens CVCL_0060
3LL Malignant tumors of the mouse pulmonary system Mus musculus CVCL_5653
HCC827 Lung adenocarcinoma Homo sapiens CVCL_2063
Calu-1 Lung squamous cell carcinoma Homo sapiens CVCL_0608
BEAS-2B Normal Homo sapiens CVCL_0168
A-549 Lung adenocarcinoma Homo sapiens CVCL_0023
A-427 Lung adenocarcinoma Homo sapiens CVCL_1055
16HBE14o- Normal Homo sapiens CVCL_0112
In-vivo Model KP and Mettl3-/- mice were bred to generate KPM-/- mice. Afterwards, the KP and KPM-/- mice were intranasally infected under anesthesia with adeno-associated virus type 5 (AAV5) expressing Cre to initiate lung tumorigenesis along with ALKBH5-expressing AAV5 or Empty AAV5 to generate KPE, KPA, KPEM-/- and KPAM-/- spontaneous LUAD mouse models. For generation of LLC-based intra-pulmonary tumor mouse models, 1 × 107 LLC cells were injected into C57BL/6 mice via the tail vein.For cell-derived xenograft (CDX) mouse models, 1.0 × 107 H1299 or 1.5 × 107 H1975 cells were subcutaneously injected into 4-6-week-old athymic nude mice. The tumors were monitored at indicated time points and isolated for further analysis after sacrifice.
RNA demethylase ALKBH5 (ALKBH5) [ERASER]
 Regulator Info 
Representative RNA-seq result indicating the expression of this target gene regulated by ALKBH5
Cell Line human pluripotent stem cells Homo sapiens
Treatment: hILO ALKBH5knockout cells
Control: hILO wild type cells
 GSE163945
Regulation
logFC: -9.52E-01
p-value: 3.98E-04
More Results Click to View More RNA-seq Results
In total 1 item(s) under this regulator
Experiment 1 Reporting the m6A Methylation Regulator of This Target Gene [1]
Response Summary Alpha-enolase (ENO1) positively correlated with METTL3 and global m6A levels, and negatively correlated with ALKBH5 in human Lung adenocarcinoma(LUAD). In addition, m6A-dependent elevation of ENO1 was associated with LUAD progression.
Target Regulation Down regulation
Responsed Disease Lung adenocarcinoma ICD-11: 2C25.0
 Disease Info 
Pathway Response Carbon metabolism hsa01200
Glycolysis / Gluconeogenesis hsa00010
Cell Process Glycolysis
In-vitro Model PC-9 Lung adenocarcinoma Homo sapiens CVCL_B260
NCI-H441 Lung papillary adenocarcinoma Homo sapiens CVCL_1561
NCI-H292 Lung mucoepidermoid carcinoma Homo sapiens CVCL_0455
NCI-H2030 Lung adenocarcinoma Homo sapiens CVCL_1517
NCI-H1975 Lung adenocarcinoma Homo sapiens CVCL_1511
NCI-H1650 Minimally invasive lung adenocarcinoma Homo sapiens CVCL_1483
NCI-H1299 Lung large cell carcinoma Homo sapiens CVCL_0060
3LL Malignant tumors of the mouse pulmonary system Mus musculus CVCL_5653
HCC827 Lung adenocarcinoma Homo sapiens CVCL_2063
Calu-1 Lung squamous cell carcinoma Homo sapiens CVCL_0608
BEAS-2B Normal Homo sapiens CVCL_0168
A-549 Lung adenocarcinoma Homo sapiens CVCL_0023
A-427 Lung adenocarcinoma Homo sapiens CVCL_1055
16HBE14o- Normal Homo sapiens CVCL_0112
In-vivo Model KP and Mettl3-/- mice were bred to generate KPM-/- mice. Afterwards, the KP and KPM-/- mice were intranasally infected under anesthesia with adeno-associated virus type 5 (AAV5) expressing Cre to initiate lung tumorigenesis along with ALKBH5-expressing AAV5 or Empty AAV5 to generate KPE, KPA, KPEM-/- and KPAM-/- spontaneous LUAD mouse models. For generation of LLC-based intra-pulmonary tumor mouse models, 1 × 107 LLC cells were injected into C57BL/6 mice via the tail vein.For cell-derived xenograft (CDX) mouse models, 1.0 × 107 H1299 or 1.5 × 107 H1975 cells were subcutaneously injected into 4-6-week-old athymic nude mice. The tumors were monitored at indicated time points and isolated for further analysis after sacrifice.
Wilms tumor 1-associating protein (WTAP) [WRITER]
 Regulator Info 
Representative RNA-seq result indicating the expression of this target gene regulated by WTAP
Cell Line Human umbilical vein endothelial cells Homo sapiens
Treatment: siWTAP HUVECs
Control: siControl HUVECs
 GSE167067
Regulation
logFC: -6.99E-01
p-value: 1.98E-02
More Results Click to View More RNA-seq Results
In total 1 item(s) under this regulator
Experiment 1 Reporting the m6A Methylation Regulator of This Target Gene [2]
Response Summary The stabilization of WTAP further promotes RNA m6A methylation of Alpha-enolase (ENO1), impacting the glycolytic activity of BC cells.
Target Regulation Up regulation
Responsed Disease Breast cancer ICD-11: 2C60
 Disease Info 
Pathway Response Glycolysis / Gluconeogenesis hsa00010
Cell Process Glycolysis
In-vitro Model MDA-MB-231 Breast adenocarcinoma Homo sapiens CVCL_0062
MCF-7 Invasive breast carcinoma Homo sapiens CVCL_0031
HEK293T Normal Homo sapiens CVCL_0063
In-vivo Model A total of 1 × 106 luciferase-labeled BC cells transfected with shWTAP or shNC were injected subcutaneously with or without C5aR1 neutrophils (tumor cells:neutrophils, 10:1).
Lung cancer [ICD-11: 2C25]
 Disease Info 
In total 2 item(s) under this disease
Experiment 1 Reporting the m6A-centered Disease Response [1]
Response Summary Alpha-enolase (ENO1) positively correlated with METTL3 and global m6A levels, and negatively correlated with ALKBH5 in human Lung adenocarcinoma(LUAD). In addition, m6A-dependent elevation of ENO1 was associated with LUAD progression.
Responsed Disease Lung adenocarcinoma [ICD-11: 2C25.0]
Target Regulator Methyltransferase-like 3 (METTL3) WRITER
 Regulator Info 
Target Regulation Up regulation
Pathway Response Carbon metabolism hsa01200
Glycolysis / Gluconeogenesis hsa00010
Cell Process Glycolysis
In-vitro Model PC-9 Lung adenocarcinoma Homo sapiens CVCL_B260
NCI-H441 Lung papillary adenocarcinoma Homo sapiens CVCL_1561
NCI-H292 Lung mucoepidermoid carcinoma Homo sapiens CVCL_0455
NCI-H2030 Lung adenocarcinoma Homo sapiens CVCL_1517
NCI-H1975 Lung adenocarcinoma Homo sapiens CVCL_1511
NCI-H1650 Minimally invasive lung adenocarcinoma Homo sapiens CVCL_1483
NCI-H1299 Lung large cell carcinoma Homo sapiens CVCL_0060
3LL Malignant tumors of the mouse pulmonary system Mus musculus CVCL_5653
HCC827 Lung adenocarcinoma Homo sapiens CVCL_2063
Calu-1 Lung squamous cell carcinoma Homo sapiens CVCL_0608
BEAS-2B Normal Homo sapiens CVCL_0168
A-549 Lung adenocarcinoma Homo sapiens CVCL_0023
A-427 Lung adenocarcinoma Homo sapiens CVCL_1055
16HBE14o- Normal Homo sapiens CVCL_0112
In-vivo Model KP and Mettl3-/- mice were bred to generate KPM-/- mice. Afterwards, the KP and KPM-/- mice were intranasally infected under anesthesia with adeno-associated virus type 5 (AAV5) expressing Cre to initiate lung tumorigenesis along with ALKBH5-expressing AAV5 or Empty AAV5 to generate KPE, KPA, KPEM-/- and KPAM-/- spontaneous LUAD mouse models. For generation of LLC-based intra-pulmonary tumor mouse models, 1 × 107 LLC cells were injected into C57BL/6 mice via the tail vein.For cell-derived xenograft (CDX) mouse models, 1.0 × 107 H1299 or 1.5 × 107 H1975 cells were subcutaneously injected into 4-6-week-old athymic nude mice. The tumors were monitored at indicated time points and isolated for further analysis after sacrifice.
Experiment 2 Reporting the m6A-centered Disease Response [1]
Response Summary Alpha-enolase (ENO1) positively correlated with METTL3 and global m6A levels, and negatively correlated with ALKBH5 in human Lung adenocarcinoma(LUAD). In addition, m6A-dependent elevation of ENO1 was associated with LUAD progression.
Responsed Disease Lung adenocarcinoma [ICD-11: 2C25.0]
Target Regulator RNA demethylase ALKBH5 (ALKBH5) ERASER
 Regulator Info 
Target Regulation Down regulation
Pathway Response Carbon metabolism hsa01200
Glycolysis / Gluconeogenesis hsa00010
Cell Process Glycolysis
In-vitro Model PC-9 Lung adenocarcinoma Homo sapiens CVCL_B260
NCI-H441 Lung papillary adenocarcinoma Homo sapiens CVCL_1561
NCI-H292 Lung mucoepidermoid carcinoma Homo sapiens CVCL_0455
NCI-H2030 Lung adenocarcinoma Homo sapiens CVCL_1517
NCI-H1975 Lung adenocarcinoma Homo sapiens CVCL_1511
NCI-H1650 Minimally invasive lung adenocarcinoma Homo sapiens CVCL_1483
NCI-H1299 Lung large cell carcinoma Homo sapiens CVCL_0060
3LL Malignant tumors of the mouse pulmonary system Mus musculus CVCL_5653
HCC827 Lung adenocarcinoma Homo sapiens CVCL_2063
Calu-1 Lung squamous cell carcinoma Homo sapiens CVCL_0608
BEAS-2B Normal Homo sapiens CVCL_0168
A-549 Lung adenocarcinoma Homo sapiens CVCL_0023
A-427 Lung adenocarcinoma Homo sapiens CVCL_1055
16HBE14o- Normal Homo sapiens CVCL_0112
In-vivo Model KP and Mettl3-/- mice were bred to generate KPM-/- mice. Afterwards, the KP and KPM-/- mice were intranasally infected under anesthesia with adeno-associated virus type 5 (AAV5) expressing Cre to initiate lung tumorigenesis along with ALKBH5-expressing AAV5 or Empty AAV5 to generate KPE, KPA, KPEM-/- and KPAM-/- spontaneous LUAD mouse models. For generation of LLC-based intra-pulmonary tumor mouse models, 1 × 107 LLC cells were injected into C57BL/6 mice via the tail vein.For cell-derived xenograft (CDX) mouse models, 1.0 × 107 H1299 or 1.5 × 107 H1975 cells were subcutaneously injected into 4-6-week-old athymic nude mice. The tumors were monitored at indicated time points and isolated for further analysis after sacrifice.
Breast cancer [ICD-11: 2C60]
 Disease Info 
In total 1 item(s) under this disease
Experiment 1 Reporting the m6A-centered Disease Response [2]
Response Summary The stabilization of WTAP further promotes RNA m6A methylation of Alpha-enolase (ENO1), impacting the glycolytic activity of BC cells.
Responsed Disease Breast cancer [ICD-11: 2C60]
Target Regulator Wilms tumor 1-associating protein (WTAP) WRITER
 Regulator Info 
Target Regulation Up regulation
Pathway Response Glycolysis / Gluconeogenesis hsa00010
Cell Process Glycolysis
In-vitro Model MDA-MB-231 Breast adenocarcinoma Homo sapiens CVCL_0062
MCF-7 Invasive breast carcinoma Homo sapiens CVCL_0031
HEK293T Normal Homo sapiens CVCL_0063
In-vivo Model A total of 1 × 106 luciferase-labeled BC cells transfected with shWTAP or shNC were injected subcutaneously with or without C5aR1 neutrophils (tumor cells:neutrophils, 10:1).
References
Ref 1 The essential roles of m(6)A RNA modification to stimulate ENO1-dependent glycolysis and tumorigenesis in lung adenocarcinoma. J Exp Clin Cancer Res. 2022 Jan 25;41(1):36. doi: 10.1186/s13046-021-02200-5.
Ref 2 C5aR1-positive neutrophils promote breast cancer glycolysis through WTAP-dependent m6A methylation of ENO1. Cell Death Dis. 2021 Jul 26;12(8):737. doi: 10.1038/s41419-021-04028-5.