m6A Target Gene Information

General Information of the m6A Target Gene (ID: M6ATAR00431)
Target Name Metalloproteinase inhibitor 3 (TIMP3)
Synonyms
Protein MIG-5; Tissue inhibitor of metalloproteinases 3; TIMP-3
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Gene Name TIMP3
Chromosomal Location 22q12.3
Family protease inhibitor I35 (TIMP) family
Function
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15.
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Gene ID 7078
Uniprot ID
TIMP3_HUMAN
HGNC ID
HGNC:11822
Ensembl Gene ID
ENSG00000100234
KEGG ID
hsa:7078
Full List of m6A Methylation Regulator of This Target Gene and Corresponding Disease/Drug Response(s)
TIMP3 can be regulated by the following regulator(s), and cause disease/drug response(s). You can browse detail information of regulator(s) or disease/drug response(s).
Browse Regulator
Browse Disease
RNA demethylase ALKBH5 (ALKBH5) [ERASER]
 Regulator Info 
Representative RNA-seq result indicating the expression of this target gene regulated by ALKBH5
Cell Line HaCAT cell line Homo sapiens
Treatment: siALKBH5 HaCAT cells
Control: siControl HaCAT cells
 GSE211076
Regulation
logFC: -5.86E-01
p-value: 2.60E-20
More Results Click to View More RNA-seq Results
In total 2 item(s) under this regulator
Experiment 1 Reporting the m6A Methylation Regulator of This Target Gene [1]
Response Summary ALKBH5 repress Metalloproteinase inhibitor 3 (TIMP3) transcript stability, thereby inhibiting TIMP3 translational production.the present research confirmed the ALKBH5/TIMP3 pathway in the non-small cell lung cancer(NSCLC) oncogenesis progress, providing a novel insight for the epitranscriptome and potential therapeutic target for NSCLC.
Target Regulation Down regulation
Responsed Disease Non-small-cell lung carcinoma ICD-11: 2C25.Y
 Disease Info 
Cell Process RNA stability
Cell apoptosis
In-vitro Model A-549 Lung adenocarcinoma Homo sapiens CVCL_0023
NCI-H1299 Lung large cell carcinoma Homo sapiens CVCL_0060
NCI-H460 Lung large cell carcinoma Homo sapiens CVCL_0459
NHBE (Normal bronchial epithelial cells)
SK-MES-1 Lung squamous cell carcinoma Homo sapiens CVCL_0630
In-vivo Model 2 × 106 A549 cells stably transfected with shRNA-ALKBH5 were injected into the flank of male athymic BALB/c nude mice (4-5 weeks old, 10 mice).
Experiment 2 Reporting the m6A Methylation Regulator of This Target Gene [2]
Response Summary The expression of CDKN1A (p21) or Metalloproteinase inhibitor 3 (TIMP3) was increased by ALKBH5 knockdown. In conclusions, the ALKBH5-IGF2BPs axis promotes cell proliferation and tumorigenicity, which in turn causes the unfavorable prognosis of NSCLC.
Target Regulation Down regulation
Responsed Disease Non-small-cell lung carcinoma ICD-11: 2C25.Y
 Disease Info 
Pathway Response Cell cycle hsa04110
Cell Process Arrest cell cycle at G1 phase
In-vitro Model NCI-H1299 Lung large cell carcinoma Homo sapiens CVCL_0060
NCI-H460 Lung large cell carcinoma Homo sapiens CVCL_0459
NCI-H2087 Lung adenocarcinoma Homo sapiens CVCL_1524
A-549 Lung adenocarcinoma Homo sapiens CVCL_0023
ABC-1 Lung adenocarcinoma Homo sapiens CVCL_1066
NCI-H358 Minimally invasive lung adenocarcinoma Homo sapiens CVCL_1559
BEAS-2B Normal Homo sapiens CVCL_0168
HEK293 Normal Homo sapiens CVCL_0045
PC-3 [Human lung carcinoma] Lung adenocarcinoma Homo sapiens CVCL_S982
PC-9 Lung adenocarcinoma Homo sapiens CVCL_B260
RERF-LC-MS Lung adenocarcinoma Homo sapiens CVCL_1655
HLC-1 Lung adenocarcinoma Homo sapiens CVCL_5529
LC-2/ad Lung adenocarcinoma Homo sapiens CVCL_1373
Lung cancer [ICD-11: 2C25]
 Disease Info 
In total 2 item(s) under this disease
Experiment 1 Reporting the m6A-centered Disease Response [1]
Response Summary ALKBH5 repress Metalloproteinase inhibitor 3 (TIMP3) transcript stability, thereby inhibiting TIMP3 translational production.the present research confirmed the ALKBH5/TIMP3 pathway in the non-small cell lung cancer(NSCLC) oncogenesis progress, providing a novel insight for the epitranscriptome and potential therapeutic target for NSCLC.
Responsed Disease Non-small-cell lung carcinoma [ICD-11: 2C25.Y]
Target Regulator RNA demethylase ALKBH5 (ALKBH5) ERASER
 Regulator Info 
Target Regulation Down regulation
Cell Process RNA stability
Cell apoptosis
In-vitro Model A-549 Lung adenocarcinoma Homo sapiens CVCL_0023
NCI-H1299 Lung large cell carcinoma Homo sapiens CVCL_0060
NCI-H460 Lung large cell carcinoma Homo sapiens CVCL_0459
NHBE (Normal bronchial epithelial cells)
SK-MES-1 Lung squamous cell carcinoma Homo sapiens CVCL_0630
In-vivo Model 2 × 106 A549 cells stably transfected with shRNA-ALKBH5 were injected into the flank of male athymic BALB/c nude mice (4-5 weeks old, 10 mice).
Experiment 2 Reporting the m6A-centered Disease Response [2]
Response Summary The expression of CDKN1A (p21) or Metalloproteinase inhibitor 3 (TIMP3) was increased by ALKBH5 knockdown. In conclusions, the ALKBH5-IGF2BPs axis promotes cell proliferation and tumorigenicity, which in turn causes the unfavorable prognosis of NSCLC.
Responsed Disease Non-small-cell lung carcinoma [ICD-11: 2C25.Y]
Target Regulator RNA demethylase ALKBH5 (ALKBH5) ERASER
 Regulator Info 
Target Regulation Down regulation
Pathway Response Cell cycle hsa04110
Cell Process Arrest cell cycle at G1 phase
In-vitro Model NCI-H1299 Lung large cell carcinoma Homo sapiens CVCL_0060
NCI-H460 Lung large cell carcinoma Homo sapiens CVCL_0459
NCI-H2087 Lung adenocarcinoma Homo sapiens CVCL_1524
A-549 Lung adenocarcinoma Homo sapiens CVCL_0023
ABC-1 Lung adenocarcinoma Homo sapiens CVCL_1066
NCI-H358 Minimally invasive lung adenocarcinoma Homo sapiens CVCL_1559
BEAS-2B Normal Homo sapiens CVCL_0168
HEK293 Normal Homo sapiens CVCL_0045
PC-3 [Human lung carcinoma] Lung adenocarcinoma Homo sapiens CVCL_S982
PC-9 Lung adenocarcinoma Homo sapiens CVCL_B260
RERF-LC-MS Lung adenocarcinoma Homo sapiens CVCL_1655
HLC-1 Lung adenocarcinoma Homo sapiens CVCL_5529
LC-2/ad Lung adenocarcinoma Homo sapiens CVCL_1373
References
Ref 1 N(6)-methyladenosine ALKBH5 promotes non-small cell lung cancer progress by regulating TIMP3 stability. Gene. 2020 Mar 20;731:144348. doi: 10.1016/j.gene.2020.144348. Epub 2020 Jan 9.
Ref 2 m(6)A demethylase ALKBH5 promotes tumor cell proliferation by destabilizing IGF2BPs target genes and worsens the prognosis of patients with non-small-cell lung cancer. Cancer Gene Ther. 2022 Mar 22. doi: 10.1038/s41417-022-00451-8. Online ahead of print.