m6A Target Gene Information

General Information of the m6A Target Gene (ID: M6ATAR00374)
Target Name Protein patched homolog 1 (PTCH1)
Synonyms
PTC; PTC1; PTCH
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Gene Name PTCH1
Chromosomal Location 9q22.32
Family patched family
Function
Acts as a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). Associates with the smoothened protein (SMO) to transduce the hedgehog's proteins signal. Seems to have a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis.
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Gene ID 5727
Uniprot ID
PTC1_HUMAN
HGNC ID
HGNC:9585
Ensembl Gene ID
ENSG00000185920
KEGG ID
hsa:5727
Full List of m6A Methylation Regulator of This Target Gene and Corresponding Disease/Drug Response(s)
PTCH1 can be regulated by the following regulator(s), and cause disease/drug response(s). You can browse detail information of regulator(s) or disease/drug response(s).
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RNA demethylase ALKBH5 (ALKBH5) [ERASER]
 Regulator Info 
Representative RNA-seq result indicating the expression of this target gene regulated by ALKBH5
Cell Line 143B cell line Homo sapiens
Treatment: siALKBH5 transfected 143B cells
Control: siControl 143B cells
 GSE154528
Regulation
logFC: -7.48E-01
p-value: 6.04E-03
More Results Click to View More RNA-seq Results
In total 1 item(s) under this regulator
Experiment 1 Reporting the m6A Methylation Regulator of This Target Gene [1]
Response Summary ALKBH5 mediated Protein patched homolog 1 (PTCH1) activation via a m6A-dependent manner,ALKBH5 ameliorated liver fibrosis and suppressed HSCs activation via triggering PTCH1 activation in a m6A dependent manner.
Target Regulation Up regulation
Responsed Disease Hepatic fibrosis ICD-11: DB93.0
 Disease Info 
Pathway Response Hedgehog signaling pathway hsa04340
In-vitro Model HSCs (Hepatic stellate cells(HSCs) were purchased from American Type Culture Collections (Manassas, VA))
In-vivo Model Liver fibrosis mice model was induced by intraperitoneal CCl4 injection for 12 weeks. The dose regimen for CCl4 in mice is 1 ml kg-1, diluted to 50% with olive oil twice per week for 12 weeks. Until 11 weeks, mice with liver-specific disruption of ALKBH5 were given hydrodynamic tail-vein injections of LV5-ALKBH5.
Wilms tumor 1-associating protein (WTAP) [WRITER]
 Regulator Info 
Representative RNA-seq result indicating the expression of this target gene regulated by WTAP
Cell Line mouse embryonic stem cells Mus musculus
Treatment: WTAP-/- ESCs
Control: Wild type ESCs
 GSE145309
Regulation
logFC: 1.15E+00
p-value: 6.91E-68
More Results Click to View More RNA-seq Results
In total 1 item(s) under this regulator
Experiment 1 Reporting the m6A Methylation Regulator of This Target Gene [2]
Response Summary AcSDKP in liver fibrosis via m6A modification and Hedgehog pathway, which helps us to shed light on the molecular mechanism in liver fibrosis progression. WTAP targeted the 3'-UTR of Protein patched homolog 1 (PTCH1) mRNA, and administration of AcSDKP reduced the stability of Ptch1 mRNA.
Target Regulation Down regulation
Responsed Disease Hepatic fibrosis ICD-11: DB93.0
 Disease Info 
Responsed Drug AcSDKP Phase 2
 Drug Info 
Pathway Response Hedgehog signaling pathway hsa04340
Cell Process Cell apoptosis
In-vitro Model HSC (Hematopoietic stem cell)
In-vivo Model Male Sprague-Dawley rats (375-400 g) liver fibrosis was induced by subcutaneous injection of carbon tetrachloride (CCl4) and olive oil (a ratio of 2:3) twice per week.
Hepatic fibrosis/cirrhosis [ICD-11: DB93]
 Disease Info 
In total 2 item(s) under this disease
Experiment 1 Reporting the m6A-centered Disease Response [1]
Response Summary ALKBH5 mediated Protein patched homolog 1 (PTCH1) activation via a m6A-dependent manner,ALKBH5 ameliorated liver fibrosis and suppressed HSCs activation via triggering PTCH1 activation in a m6A dependent manner.
Responsed Disease Hepatic fibrosis [ICD-11: DB93.0]
Target Regulator RNA demethylase ALKBH5 (ALKBH5) ERASER
 Regulator Info 
Target Regulation Up regulation
Pathway Response Hedgehog signaling pathway hsa04340
In-vitro Model HSCs (Hepatic stellate cells(HSCs) were purchased from American Type Culture Collections (Manassas, VA))
In-vivo Model Liver fibrosis mice model was induced by intraperitoneal CCl4 injection for 12 weeks. The dose regimen for CCl4 in mice is 1 ml kg-1, diluted to 50% with olive oil twice per week for 12 weeks. Until 11 weeks, mice with liver-specific disruption of ALKBH5 were given hydrodynamic tail-vein injections of LV5-ALKBH5.
Experiment 2 Reporting the m6A-centered Disease Response [2]
Response Summary AcSDKP in liver fibrosis via m6A modification and Hedgehog pathway, which helps us to shed light on the molecular mechanism in liver fibrosis progression. WTAP targeted the 3'-UTR of Protein patched homolog 1 (PTCH1) mRNA, and administration of AcSDKP reduced the stability of Ptch1 mRNA.
Responsed Disease Hepatic fibrosis [ICD-11: DB93.0]
Target Regulator Wilms tumor 1-associating protein (WTAP) WRITER
 Regulator Info 
Target Regulation Down regulation
Responsed Drug AcSDKP Phase 2
 Drug Info 
Pathway Response Hedgehog signaling pathway hsa04340
Cell Process Cell apoptosis
In-vitro Model HSC (Hematopoietic stem cell)
In-vivo Model Male Sprague-Dawley rats (375-400 g) liver fibrosis was induced by subcutaneous injection of carbon tetrachloride (CCl4) and olive oil (a ratio of 2:3) twice per week.
AcSDKP [Phase 2]
 Drug Info 
In total 1 item(s) under this drug
Experiment 1 Reporting the m6A-centered Drug Response [2]
Response Summary AcSDKP in liver fibrosis via m6A modification and Hedgehog pathway, which helps us to shed light on the molecular mechanism in liver fibrosis progression. WTAP targeted the 3'-UTR of Protein patched homolog 1 (PTCH1) mRNA, and administration of AcSDKP reduced the stability of Ptch1 mRNA.
Target Regulator Wilms tumor 1-associating protein (WTAP) WRITER
 Regulator Info 
Target Regulation Down regulation
Responsed Disease Hepatic fibrosis ICD-11: DB93.0
 Disease Info 
Pathway Response Hedgehog signaling pathway hsa04340
Cell Process Cell apoptosis
In-vitro Model HSC (Hematopoietic stem cell)
In-vivo Model Male Sprague-Dawley rats (375-400 g) liver fibrosis was induced by subcutaneous injection of carbon tetrachloride (CCl4) and olive oil (a ratio of 2:3) twice per week.
References
Ref 1 ALKBH5 ameliorated liver fibrosis and suppressed HSCs activation via triggering PTCH1 activation in an m(6)A dependent manner. Eur J Pharmacol. 2022 May 5;922:174900. doi: 10.1016/j.ejphar.2022.174900. Epub 2022 Mar 19.
Ref 2 N-acetyl-seryl-aspartyl-lysyl-proline (AcSDKP) mitigates the liver fibrosis via WTAP/m(6)A/Ptch1 axis through Hedgehog pathway. Gene. 2022 Mar 1;813:146125. doi: 10.1016/j.gene.2021.146125. Epub 2021 Dec 16.