m6A Target Gene Information

General Information of the m6A Target Gene (ID: M6ATAR00112)
Target Name ZNFX1 antisense RNA 1 (ZFAS1)
Synonyms
ZFAS1; C20orf199NCRNA00275ZNFX1-AS1; chromosome 20 open reading frame 199; non-protein coding RNA 275; ZNFX1 antisense RNA 1 (non-protein coding); HSUP1; HSUP2
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Gene Name ZFAS1
Chromosomal Location 20q13.13
Family Small nucleolar RNA non-coding host genes; Long non-coding RNAs with non-systematic symbols
Gene ID 441951
HGNC ID
HGNC:33101
Ensembl Gene ID
ENSG00000177410
Full List of m6A Methylation Regulator of This Target Gene and Corresponding Disease/Drug Response(s)
ZFAS1 can be regulated by the following regulator(s), and cause disease/drug response(s). You can browse detail information of regulator(s) or disease/drug response(s).
Browse Regulator
Browse Disease
Methyltransferase-like 3 (METTL3) [WRITER]
 Regulator Info 
Representative RIP-seq result supporting the interaction between ZFAS1 and the regulator
Cell Line MDA-MB-231 Homo sapiens
Regulation logFC: 3.61E+00 GSE60213
In total 2 item(s) under this regulator
Experiment 1 Reporting the m6A Methylation Regulator of This Target Gene [1]
Response Summary Knockout of METTL3 can reduce the total expression of ZNFX1 antisense RNA 1 (ZFAS1). ZFAS1 is used as an oncogenic lncRNA, which can promote NPCcell proliferation, migration and tumor growth.
Target Regulation Up regulation
Responsed Disease Nasopharyngeal carcinoma ICD-11: 2B6B
 Disease Info 
Pathway Response PI3K-Akt signaling pathway hsa04151
Cell Process Cell proliferation
Cell migration
Cell autophagy
In-vitro Model SUNE1 Nasopharyngeal carcinoma Homo sapiens CVCL_6946
S26 Nasopharyngeal carcinoma Homo sapiens CVCL_B0UB
S18 Nasopharyngeal carcinoma Homo sapiens CVCL_B0U9
NP69 (A human immortalized nasopharyngeal epithelial)
N2Tert (The human immortalized nasopharyngeal epithelial cell lines)
HONE-1 Nasopharyngeal carcinoma Homo sapiens CVCL_8706
HNE-1 Nasopharyngeal carcinoma Homo sapiens CVCL_0308
NPC/HK1 Nasopharyngeal carcinoma Homo sapiens CVCL_7084
CNE-2 Nasopharyngeal carcinoma Homo sapiens CVCL_6889
CNE-1 Normal Homo sapiens CVCL_6888
C666-1 Nasopharyngeal carcinoma Homo sapiens CVCL_7949
6-10B Nasopharyngeal carcinoma Homo sapiens CVCL_C529
In-vivo Model Female BALB/c nude mice (ages 4-5 weeks, 18-20 g) were purchased from the Charles River Laboratories. For the tumor growth model, 1 × 106 HONE-1 sh-NC or sh-ZFAS1 cells were injected into the axilla of the mice, and the tumor size was measured every 3 days. On day 30, the mice were killed, and the tumors were dissected and weighed.
Experiment 2 Reporting the m6A Methylation Regulator of This Target Gene [2]
Response Summary ZNFX1 antisense RNA 1 (ZFAS1) sequestered miR-647, and this RNA-RNA interaction is regulated by METLL3-mediated m6A modification in cervical cancer.
Responsed Disease Cervical cancer ICD-11: 2C77
 Disease Info 
Cell Process Cell proliferation
Cell migration
Cell invasion
In-vitro Model HEK293T Normal Homo sapiens CVCL_0063
C-33 A Cervical squamous cell carcinoma Homo sapiens CVCL_1094
Ca Ski Cervical squamous cell carcinoma Homo sapiens CVCL_1100
HeLa Endocervical adenocarcinoma Homo sapiens CVCL_0030
SiHa Cervical squamous cell carcinoma Homo sapiens CVCL_0032
In-vivo Model Nude mice were subjected to a subcutaneous injection of 5× 106 control and ZFAS1 silencing CaSki cells suspended in 0.2 mL DMEM medium.
Insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2) [READER]
 Regulator Info 
In total 1 item(s) under this regulator
Experiment 1 Reporting the m6A Methylation Regulator of This Target Gene [3]
Response Summary The critical modulation network underlying m6A readers stabilizes lncRNAs, and they jointly promote mitochondrial energy metabolism in the pathogenesis of colorectal cancer. N6-methyladenosine reader stabilizes the ZFAS1/OLA1 axis. Thus, direct interaction between the KH3-4 domain of IMP2 and ZFAS1 where IMP2 serves as a reader for m6A-modified ZFAS1 and promotes the RNA stability of ZFAS1 is critical for CRC development.
Target Regulation Up regulation
Responsed Disease Colorectal cancer ICD-11: 2B91
 Disease Info 
Cell Process Warburg effect
Mitochondrial energy metabolism
In-vitro Model Caco-2 Colon adenocarcinoma Homo sapiens CVCL_0025
HCT 116 Colon carcinoma Homo sapiens CVCL_0291
HIEC (Normal intestinal epithelial cells)
HT29 Colon cancer Mus musculus CVCL_A8EZ
LoVo Colon adenocarcinoma Homo sapiens CVCL_0399
RKO Colon carcinoma Homo sapiens CVCL_0504
SW480 Colon adenocarcinoma Homo sapiens CVCL_0546
SW480 Colon adenocarcinoma Homo sapiens CVCL_0546
SW620 Colon adenocarcinoma Homo sapiens CVCL_0547
Nasopharyngeal carcinoma [ICD-11: 2B6B]
 Disease Info 
In total 1 item(s) under this disease
Experiment 1 Reporting the m6A-centered Disease Response [1]
Response Summary Knockout of METTL3 can reduce the total expression of ZNFX1 antisense RNA 1 (ZFAS1). ZFAS1 is used as an oncogenic lncRNA, which can promote NPCcell proliferation, migration and tumor growth.
Responsed Disease Nasopharyngeal carcinoma [ICD-11: 2B6B]
Target Regulator Methyltransferase-like 3 (METTL3) WRITER
 Regulator Info 
Target Regulation Up regulation
Pathway Response PI3K-Akt signaling pathway hsa04151
Cell Process Cell proliferation
Cell migration
Cell autophagy
In-vitro Model SUNE1 Nasopharyngeal carcinoma Homo sapiens CVCL_6946
S26 Nasopharyngeal carcinoma Homo sapiens CVCL_B0UB
S18 Nasopharyngeal carcinoma Homo sapiens CVCL_B0U9
NP69 (A human immortalized nasopharyngeal epithelial)
N2Tert (The human immortalized nasopharyngeal epithelial cell lines)
HONE-1 Nasopharyngeal carcinoma Homo sapiens CVCL_8706
HNE-1 Nasopharyngeal carcinoma Homo sapiens CVCL_0308
NPC/HK1 Nasopharyngeal carcinoma Homo sapiens CVCL_7084
CNE-2 Nasopharyngeal carcinoma Homo sapiens CVCL_6889
CNE-1 Normal Homo sapiens CVCL_6888
C666-1 Nasopharyngeal carcinoma Homo sapiens CVCL_7949
6-10B Nasopharyngeal carcinoma Homo sapiens CVCL_C529
In-vivo Model Female BALB/c nude mice (ages 4-5 weeks, 18-20 g) were purchased from the Charles River Laboratories. For the tumor growth model, 1 × 106 HONE-1 sh-NC or sh-ZFAS1 cells were injected into the axilla of the mice, and the tumor size was measured every 3 days. On day 30, the mice were killed, and the tumors were dissected and weighed.
Colorectal cancer [ICD-11: 2B91]
 Disease Info 
In total 1 item(s) under this disease
Experiment 1 Reporting the m6A-centered Disease Response [3]
Response Summary The critical modulation network underlying m6A readers stabilizes lncRNAs, and they jointly promote mitochondrial energy metabolism in the pathogenesis of colorectal cancer. N6-methyladenosine reader stabilizes the ZFAS1/OLA1 axis. Thus, direct interaction between the KH3-4 domain of IMP2 and ZFAS1 where IMP2 serves as a reader for m6A-modified ZFAS1 and promotes the RNA stability of ZFAS1 is critical for CRC development.
Responsed Disease Colorectal cancer [ICD-11: 2B91]
Target Regulator Insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2) READER
 Regulator Info 
Target Regulation Up regulation
Cell Process Warburg effect
Mitochondrial energy metabolism
In-vitro Model Caco-2 Colon adenocarcinoma Homo sapiens CVCL_0025
HCT 116 Colon carcinoma Homo sapiens CVCL_0291
HIEC (Normal intestinal epithelial cells)
HT29 Colon cancer Mus musculus CVCL_A8EZ
LoVo Colon adenocarcinoma Homo sapiens CVCL_0399
RKO Colon carcinoma Homo sapiens CVCL_0504
SW480 Colon adenocarcinoma Homo sapiens CVCL_0546
SW480 Colon adenocarcinoma Homo sapiens CVCL_0546
SW620 Colon adenocarcinoma Homo sapiens CVCL_0547
Cervical cancer [ICD-11: 2C77]
 Disease Info 
In total 1 item(s) under this disease
Experiment 1 Reporting the m6A-centered Disease Response [2]
Response Summary ZNFX1 antisense RNA 1 (ZFAS1) sequestered miR-647, and this RNA-RNA interaction is regulated by METLL3-mediated m6A modification in cervical cancer.
Responsed Disease Cervical cancer [ICD-11: 2C77]
Target Regulator Methyltransferase-like 3 (METTL3) WRITER
 Regulator Info 
Cell Process Cell proliferation
Cell migration
Cell invasion
In-vitro Model HEK293T Normal Homo sapiens CVCL_0063
C-33 A Cervical squamous cell carcinoma Homo sapiens CVCL_1094
Ca Ski Cervical squamous cell carcinoma Homo sapiens CVCL_1100
HeLa Endocervical adenocarcinoma Homo sapiens CVCL_0030
SiHa Cervical squamous cell carcinoma Homo sapiens CVCL_0032
In-vivo Model Nude mice were subjected to a subcutaneous injection of 5× 106 control and ZFAS1 silencing CaSki cells suspended in 0.2 mL DMEM medium.
References
Ref 1 The m6A methyltransferase METTL3 affects autophagy and progression of nasopharyngeal carcinoma by regulating the stability of lncRNA ZFAS1. Infect Agent Cancer. 2022 Jan 3;17(1):1. doi: 10.1186/s13027-021-00411-1.
Ref 2 ZFAS1 Exerts an Oncogenic Role via Suppressing miR-647 in an m(6)A-Dependent Manner in Cervical Cancer. Onco Targets Ther. 2020 Nov 17;13:11795-11806. doi: 10.2147/OTT.S274492. eCollection 2020.
Ref 3 N6-methyladenosine reader IMP2 stabilizes the ZFAS1/OLA1 axis and activates the Warburg effect: implication in colorectal cancer. J Hematol Oncol. 2021 Nov 7;14(1):188. doi: 10.1186/s13045-021-01204-0.
Ref 4 METTL14 mediated m6A modification to LncRNA ZFAS1/ RAB22A: A novel therapeutic target for atherosclerosis. Int J Cardiol. 2021 Apr 1;328:177. doi: 10.1016/j.ijcard.2020.12.002. Epub 2020 Dec 8.