m6A-centered Disease Response Information
General Information of the Disease (ID: M6ADIS0101)
| Name |
Congestive heart failure
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|---|---|---|---|---|---|
| ICD |
ICD-11: BD10
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Full List of Target Gene(s) of This m6A-centered Disease Response
Myosin-7 (Myh7)
| In total 1 item(s) under this target gene | ||||
| Experiment 1 Reporting the m6A-centered Disease Response by This Target Gene | [1] | |||
| Response Summary | Pathological cardiac hypertrophy is a major contributor of heart failure (HF), the m6A Reader YTHDF2 suppresses cardiac hypertrophy via Myosin-7 (Myh7) mRNA decoy in an m6A-dependent manner. | |||
| Responsed Disease | Congestive heart failure [ICD-11: BD10] | |||
| Target Regulator | YTH domain-containing family protein 2 (YTHDF2) | READER | ||
| Target Regulation | Up regulation | |||
| In-vitro Model | Neonatal rat ventricular cardiomyocytes (Primary myocyte cells) | |||
| In-vivo Model | Mice were anesthetized with 0.3% sodium pentobarbital (75 mg×kg-1) intraperitoneally, and the aortic arch was tied with a 6-0 nylon suture between the brachiocephalic and left common artery with a homemade L-shaped 26G cushion needle. After ligation, the needle was quickly removed, and the skin was closed. The sham operation was identical, except that the thread was not ligated. Moreover, mice were injected with rAAV9 (4 × 1011 vector genomes (vg)/mouse) carrying an empty vector, YTHDF2 or YTH-del via the tail vein. | |||
Myosin heavy chain associated RNA transcript (MHRT)
| In total 1 item(s) under this target gene | ||||
| Experiment 1 Reporting the m6A-centered Disease Response by This Target Gene | [2] | |||
| Response Summary | FTO overexpression inhibits H/R-indcued apoptosis in myocardial cells by regulating m6A modification of Myosin heavy chain associated RNA transcript (MHRT). FTO is a target gene for heart failure treatment. | |||
| Responsed Disease | Congestive heart failure [ICD-11: BD10] | |||
| Target Regulator | Fat mass and obesity-associated protein (FTO) | ERASER | ||
| Target Regulation | Up regulation | |||
| Cell Process | Cell apoptosis | |||
| In-vitro Model | Mouse myocardial cell line (Isolated from heart tissue) | |||
| In-vivo Model | The mice were housed under standardized conditions and received normal diet. The mice were randomly divided into 5 groups (n = 6/each group): (1) Control group: normal mice served as control; (2) TAC-HF group: mice were subjected to transverse aortic constriction (TAC) to establish HF mouse model; (3) Sham group: sham-operated mice were subjected the same surgery without the placement of a ligature; (4) Doxorubicin-HF group: mice were intraperitoneally injected with doxorubicin to construct HF mouse model; and (5) PBS: mice were intraperitoneally injected with equal PBS as control. | |||
References