m6A Target Gene Information
General Information of the m6A Target Gene (ID: M6ATAR00636)
| Target Name | pri-miR-27 | ||||
|---|---|---|---|---|---|
| Gene Name | pri-miR-27 | ||||
Full List of m6A Methylation Regulator of This Target Gene and Corresponding Disease/Drug Response(s)
pri-miR-27
can be regulated by the following regulator(s), and cause disease/drug response(s). You can browse detail information of regulator(s) or disease/drug response(s).
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Methyltransferase-like 3 (METTL3) [WRITER]
| In total 1 item(s) under this regulator | ||||
| Experiment 1 Reporting the m6A Methylation Regulator of This Target Gene | [1] | |||
| Response Summary | METTL3 affected the growth, apoptosis, and stemness of MM cells through accelerating the stability of YY1 mRNA and the maturation of pri-miR-27 in vitro and in vivo. | |||
| Target Regulation | Down regulation | |||
| Responsed Disease | Multiple myeloma | ICD-11: 2A83.1 | ||
| Cell Process | Cell apoptosis | |||
| In-vitro Model | U266 (Human multiple myeloma cells) | |||
| RPMI-8226 | Plasma cell myeloma | Homo sapiens | CVCL_0014 | |
| NCI-H929 | Plasma cell myeloma | Homo sapiens | CVCL_1600 | |
| MM1.S | Plasma cell myeloma | Homo sapiens | CVCL_8792 | |
| In-vivo Model | BALB/C nude mice (5 weeks old, weighing 18-22 g) were fed in specific pathogen-free facilities and subcutaneously inoculated with U266 cells (1 × 106). The mice were randomly divided into 3 groups with 6 mice per group, when the tumor was measurable. Then, miR-27a-3p mimic or sh-METTL3 was injected intratumorally at an interval of 4 days a total of 4 times. Tumor volume was measured using a digital caliper every week and calculated using the formula V = 1/2 (width2 × length). | |||
Multiple myeloma [ICD-11: 2A83]
| In total 1 item(s) under this disease | ||||
| Experiment 1 Reporting the m6A-centered Disease Response | [1] | |||
| Response Summary | METTL3 affected the growth, apoptosis, and stemness of MM cells through accelerating the stability of YY1 mRNA and the maturation of pri-miR-27 in vitro and in vivo. | |||
| Responsed Disease | Multiple myeloma [ICD-11: 2A83.1] | |||
| Target Regulator | Methyltransferase-like 3 (METTL3) | WRITER | ||
| Target Regulation | Down regulation | |||
| Cell Process | Cell apoptosis | |||
| In-vitro Model | U266 (Human multiple myeloma cells) | |||
| RPMI-8226 | Plasma cell myeloma | Homo sapiens | CVCL_0014 | |
| NCI-H929 | Plasma cell myeloma | Homo sapiens | CVCL_1600 | |
| MM1.S | Plasma cell myeloma | Homo sapiens | CVCL_8792 | |
| In-vivo Model | BALB/C nude mice (5 weeks old, weighing 18-22 g) were fed in specific pathogen-free facilities and subcutaneously inoculated with U266 cells (1 × 106). The mice were randomly divided into 3 groups with 6 mice per group, when the tumor was measurable. Then, miR-27a-3p mimic or sh-METTL3 was injected intratumorally at an interval of 4 days a total of 4 times. Tumor volume was measured using a digital caliper every week and calculated using the formula V = 1/2 (width2 × length). | |||
Full List of Crosstalk(s) between m6A Modification and Epigenetic Regulation Related to This Regulator
Non-coding RNA
m6A Regulator: Methyltransferase-like 3 (METTL3)
| In total 1 item(s) under this m6A regulator | ||
| Crosstalk ID: M6ACROT05604 | ||
| Epigenetic Regulator | MicroRNA MIR27 family | |
| Crosstalk relationship | m6A → ncRNA | |
| Disease | Multiple myeloma | |