m6A Target Gene Information

General Information of the m6A Target Gene (ID: M6ATAR00315)
Target Name Lymphoid enhancer-binding factor 1 (LEF1)
Synonyms
LEF-1; T cell-specific transcription factor 1-alpha; TCF1-alpha
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Gene Name LEF1
Chromosomal Location 4q25
Family TCF/LEF family
Function
Transcription factor that binds DNA in a sequence-specific manner. Participates in the Wnt signaling pathway (By similarity). Activates transcription of target genes in the presence of CTNNB1 and EP300 (By similarity). PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Required for IL17A expressing gamma-delta T-cell maturation and development, via binding to regulator loci of BLK to modulate expression (By similarity). May play a role in hair cell differentiation and follicle morphogenesis (By similarity). [Isoform 1]: Transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells. [Isoform 3]: Lacks the CTNNB1 interaction domain and may therefore be an antagonist for Wnt signaling. [Isoform 5]: Transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells.
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Gene ID 51176
Uniprot ID
LEF1_HUMAN
HGNC ID
HGNC:6551
Ensembl Gene ID
ENSG00000138795
KEGG ID
hsa:51176
Full List of m6A Methylation Regulator of This Target Gene and Corresponding Disease/Drug Response(s)
LEF1 can be regulated by the following regulator(s), and cause disease/drug response(s). You can browse detail information of regulator(s) or disease/drug response(s).
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Browse Disease
Methyltransferase-like 3 (METTL3) [WRITER]
 Regulator Info 
Representative RNA-seq result indicating the expression of this target gene regulated by METTL3
Cell Line Caco-2 cell line Homo sapiens
Treatment: shMETTL3 Caco-2 cells
Control: shNTC Caco-2 cells
 GSE167075
Regulation
logFC: 6.28E-01
p-value: 2.52E-06
More Results Click to View More RNA-seq Results
In total 2 item(s) under this regulator
Experiment 1 Reporting the m6A Methylation Regulator of This Target Gene [1]
Response Summary METTL3 silence decreased the m6A methylation and total mRNA level of Lymphoid enhancer-binding factor 1 (LEF1),the m6A methyltransferase METTL3 promotes osteosarcoma cell progression by regulating the m6A level of LEF1 and activating Wnt/beta-catenin signaling pathway.
Target Regulation Up regulation
Responsed Disease Osteosarcoma ICD-11: 2B51
 Disease Info 
Pathway Response Wnt signaling pathway hsa04310
Cell Process Cell proliferation
Cell migration
Cell invasion
In-vitro Model HOS Osteosarcoma Homo sapiens CVCL_0312
MG-63 Osteosarcoma Homo sapiens CVCL_0426
NHOst (Normal human osteoblast cells)
SaOS-2 Osteosarcoma Homo sapiens CVCL_0548
U2OS Osteosarcoma Homo sapiens CVCL_0042
In-vivo Model The Metastatic Bone Tumor Model was established via injecting HOS cells into the right tibia of each animal.
Experiment 2 Reporting the m6A Methylation Regulator of This Target Gene [2]
Response Summary METTL3 influences the activity of the Wnt pathway through m6A methylation on Lymphoid enhancer-binding factor 1 (LEF1) mRNA, thereafter, promoting the progression of prostate cancer.
Target Regulation Up regulation
Responsed Disease Prostate cancer ICD-11: 2C82
 Disease Info 
Pathway Response Wnt signaling pathway hsa04310
Cell Process Cell activity and migratory
In-vitro Model PC-3 Prostate carcinoma Homo sapiens CVCL_0035
Osteosarcoma [ICD-11: 2B51]
 Disease Info 
In total 1 item(s) under this disease
Experiment 1 Reporting the m6A-centered Disease Response [1]
Response Summary METTL3 silence decreased the m6A methylation and total mRNA level of Lymphoid enhancer-binding factor 1 (LEF1),the m6A methyltransferase METTL3 promotes osteosarcoma cell progression by regulating the m6A level of LEF1 and activating Wnt/beta-catenin signaling pathway.
Responsed Disease Osteosarcoma [ICD-11: 2B51]
Target Regulator Methyltransferase-like 3 (METTL3) WRITER
 Regulator Info 
Target Regulation Up regulation
Pathway Response Wnt signaling pathway hsa04310
Cell Process Cell proliferation
Cell migration
Cell invasion
In-vitro Model HOS Osteosarcoma Homo sapiens CVCL_0312
MG-63 Osteosarcoma Homo sapiens CVCL_0426
NHOst (Normal human osteoblast cells)
SaOS-2 Osteosarcoma Homo sapiens CVCL_0548
U2OS Osteosarcoma Homo sapiens CVCL_0042
In-vivo Model The Metastatic Bone Tumor Model was established via injecting HOS cells into the right tibia of each animal.
Prostate cancer [ICD-11: 2C82]
 Disease Info 
In total 1 item(s) under this disease
Experiment 1 Reporting the m6A-centered Disease Response [2]
Response Summary METTL3 influences the activity of the Wnt pathway through m6A methylation on Lymphoid enhancer-binding factor 1 (LEF1) mRNA, thereafter, promoting the progression of prostate cancer.
Responsed Disease Prostate cancer [ICD-11: 2C82]
Target Regulator Methyltransferase-like 3 (METTL3) WRITER
 Regulator Info 
Target Regulation Up regulation
Pathway Response Wnt signaling pathway hsa04310
Cell Process Cell activity and migratory
In-vitro Model PC-3 Prostate carcinoma Homo sapiens CVCL_0035
References
Ref 1 The m6A methyltransferase METTL3 promotes osteosarcoma progression by regulating the m6A level of LEF1. Biochem Biophys Res Commun. 2019 Aug 27;516(3):719-725. doi: 10.1016/j.bbrc.2019.06.128. Epub 2019 Jun 26.
Ref 2 m6A methyltransferase METTL3 promotes the progression of prostate cancer via m6A-modified LEF1. Eur Rev Med Pharmacol Sci. 2020 Apr;24(7):3565-3571. doi: 10.26355/eurrev_202004_20817.