m6A-centered Disease Response Information
General Information of the Disease (ID: M6ADIS0174)
| Name |
Gastrointestinal stromal tumor
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|---|---|---|---|---|---|
| ICD |
ICD-11: 2B5B
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Full List of Target Gene(s) of This m6A-centered Disease Response
Multidrug resistance-associated protein 1 (MRP1/ABCC1)
| In total 1 item(s) under this target gene | ||||
| Experiment 1 Reporting the m6A-centered Disease Response by This Target Gene | [1] | |||
| Response Summary | METTL3, and the YTHDF1/eEF-1 complex mediate the translation of Multidrug resistance-associated protein 1 (MRP1/ABCC1) mRNA in an m6A-dependent manner to regulate the intracellular concentration of imatinib and drug resistance of gastrointestinal stromal tumor (GIST). | |||
| Responsed Disease | Gastrointestinal stromal tumour [ICD-11: 2B5B] | |||
| Responsed Drug | Imatinib | Approved | ||
| Target Regulator | Methyltransferase-like 3 (METTL3) | WRITER | ||
| Target Regulation | Up regulation | |||
| In-vitro Model | GIST-T1 | Gastrointestinal stromal tumor | Homo sapiens | CVCL_4976 |
| GIST882 | Gastrointestinal stromal tumor | Homo sapiens | CVCL_7044 | |
| In-vivo Model | For tumor growth assay, 4 × 106 logarithmically growing GIST cells were transfected with T1S-vector, T1S-METTL3, 882S-vector, or 882S-METTL3 constructs, and subcutaneously injected in 100 ul of PBS into the flank of female nude mice(4-week-old). Mice were then randomly divided into 8 groups (n = 5 in each group): (1) injected with T1S-vector-harboring cells, and treated with imatinib (600 mg/l in drinking water); (2) injected with T1S-vector-harboring cells, and treated with imatinib (600 mg/l in drinking water) and MRP1 inhibitor (100 mg/l in drinking water); (3) injected with T1S-METTL3-harboring cells, and treated with imatinib (600 mg/l in drinking water); (4) injected with T1S-METTL3-harboring cells, and treated with imatinib (600 mg/l in drinking water) and MRP1 inhibitor (100 mg/l in drinking water); (5) injected with 882S-vector-harboring cells and treated with imatinib (600 mg/l in drinking water); (6) injected with 882S-vector-harboring cells, and treated with imatinib (600 mg/l in drinking water) and MRP1 inhibitor (100 mg/l in drinking water); (7) injected with 882S-METTL3-harboring cells, and treated with imatinib (600 mg/l in drinking water); and (8) injected with 882S-METTL3-harboring cells, and treated with imatinib (600 mg/l in drinking water) and MRP1 inhibitor (100 mg/l in drinking water). | |||
Full List of Crosstalk(s) between m6A Modification and Epigenetic Regulation Related to This Disease
| In total 1 item(s) under this disease | ||
| Crosstalk ID: M6ACROT05732 | ||
| m6A Regulator | Methyltransferase-like 3 (METTL3) | |
| m6A Target | microRNA 675 (MIR675) | |
| Epigenetic Regulator | MicroRNA 675 (MIR675) | |
| Regulated Target | myosin phosphatase targeting protein 1 (MYPT1) | |
| Crosstalk relationship | m6A → ncRNA | |
References