m6A-centered Disease Response Information

General Information of the Disease (ID: M6ADIS0019)
Name
Female infertility
ICD
ICD-11: GA31
Full List of Target Gene(s) of This m6A-centered Disease Response
Intersectin-2 (ITSN2)
 Target Info 
In total 1 item(s) under this target gene
Experiment 1 Reporting the m6A-centered Disease Response by This Target Gene [2]
Response Summary METTL3 targets Intersectin-2 (ITSN2) for m6A modification and then enhances its stability to influence the oocytes meiosis. mRNA m6A modification in follicle development and coordination of RNA stabilization during oocyte growth.
Responsed Disease Female infertility [ICD-11: GA31]
Target Regulator Methyltransferase-like 3 (METTL3) WRITER
 Regulator Info 
Target Regulation Up regulation
In-vivo Model All mice described above were maintained on the C57BL/6 J background. Mice lacking Mettl3 in oocytes (referred to as Mettl3Gdf9 cKO) were generated by crossing Mettl3flox/flox mice with Gdf9-Cre mice. The Mettl3flox/flox female mice were used as the control group (referred to as WT). For the fertility test, six pairs of 6 weeks Mettl3flox/flox and Mettl3Gdf9 cKO female mice were randomly selected and continually mated to Mettl3flox/flox male mice which have been confirmed fertility for 5 months. The number of pups and litter size from each female was recorded.
References
Ref 1 TNFerade, an adenovector carrying the transgene for human tumor necrosis factor alpha, for patients with advanced solid tumors: surgical experience and long-term follow-up. Ann Surg Oncol. 2005 Oct;12(10):825-30. doi: 10.1245/ASO.2005.03.023. Epub 2005 Aug 9.
Ref 2 METTL3-mediated mRNA N(6)-methyladenosine is required for oocyte and follicle development in mice. Cell Death Dis. 2021 Oct 23;12(11):989. doi: 10.1038/s41419-021-04272-9.