m6A-centered Crosstalk Information
Mechanism of Crosstalk between m6A Modification and Epigenetic Regulation
| Crosstalk ID |
M6ACROT05917
|
[1] | |||
Non-coding RNA
piRNA-17458
WTAP
lncRNA miRNA circRNA
Direct
Enhancement
m6A modification
m6A Target
m6A Target
WTAP
Methylation
 : m6A sites
|
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| m6A Modification: | |||||
|---|---|---|---|---|---|
| m6A Regulator | Wilms tumor 1-associating protein (WTAP) | WRITER | |||
| Epigenetic Regulation that have Cross-talk with This m6A Modification: | |||||
| Epigenetic Regulation Type | Non-coding RNA (ncRNA) | ||||
| Epigenetic Regulator | piR-17458 | piRNA | View Details | ||
| Regulated Target | Pre-mRNA-splicing regulator WTAP (WTAP) | View Details | |||
| Crosstalk Relationship | ncRNA → m6A | Enhancement | |||
| Crosstalk Mechanism | ncRNAs directly impacts m6A modification through modulating the expression level of m6A regulator | ||||
| Crosstalk Summary | piR-17458 promotes tumorigenesis of CC in a WTAP-mediated m6A methylation manner. | ||||
| Responsed Disease | Cervical cancer | ICD-11: 2C77 | |||
| Cell Process | Cell migration, Cell invasion | ||||
| Cell proliferation | |||||
In-vitro Model |
Ca Ski | Cervical squamous cell carcinoma | Homo sapiens | CVCL_1100 | |
| SiHa | Cervical squamous cell carcinoma | Homo sapiens | CVCL_0032 | ||
| End1/E6E7 | Normal | Homo sapiens | CVCL_3684 | ||
| HUCECs (Human Uterine Cervical Epithelial Cells) | |||||
| In-vivo Model | A total of 5×106 Caski cells were subcutaneously injected into the left inguen of mice. When tumors were ~50 mm3±10% (day16). The mice were randomly divided into 4 groups and received piRNA-17458 mimic or piRNA-17458 inhibitor by intratumor injection for every 4 days for a total of five injections. On the 36th day, the tumor weight and lymph node metastasis of the nude mice were assessed after mice were euthanized. | ||||