m6A-centered Crosstalk Information
Mechanism of Crosstalk between m6A Modification and Epigenetic Regulation
| Crosstalk ID |
M6ACROT05827
|
[1] | |||
m6A modification
Circ_POLR2J2
Circ_POLR2J2
ZC3H13
Methylation
 : m6A sites
Direct
Enhancement
Non-coding RNA
hsa_circ_0081723
Regulated Target
lncRNA miRNA circRNA
|
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| m6A Modification: | |||||
|---|---|---|---|---|---|
| m6A Regulator | Zinc finger CCCH domain-containing protein 13 (ZC3H13) | WRITER | |||
| m6A Target | hsa_circ_0081723 (Circ_POLR2J2) | ||||
| Epigenetic Regulation that have Cross-talk with This m6A Modification: | |||||
| Epigenetic Regulation Type | Non-coding RNA (ncRNA) | ||||
| Epigenetic Regulator | hsa_circ_0081723 (Circ_POLR2J2) | circRNA | View Details | ||
| Crosstalk Relationship | m6A → ncRNA | Enhancement | |||
| Crosstalk Mechanism | m6A regulators directly modulate the functionality of ncRNAs through specific targeting ncRNA | ||||
| Crosstalk Summary | ZC3H13-mediated m6A modification of hsa_circ_0081723 (Circ_POLR2J2) promotes CC progression by modulating AMPK/p53 pathway. Our findings may contribute to the understanding of the molecular mechanisms underlying CC and offer potential therapeutic targets for clinical treatment. | ||||
| Responsed Disease | Cervical cancer | ICD-11: 2C77 | |||
| Pathway Response | p53 signaling pathway | hsa04115 | |||
| AMPK signaling pathway | hsa04152 | ||||
| Cell Process | mRNA stability | ||||
In-vitro Model |
HeLa | Endocervical adenocarcinoma | Homo sapiens | CVCL_0030 | |
| Ca Ski | Cervical squamous cell carcinoma | Homo sapiens | CVCL_1100 | ||
| In-vivo Model | A total of six female BALB/c nude mice (4-week-old) were evenly divided to sh-NC group and sh-circ group. Then, 2 × 107 HeLa cells, transfected with either sh-NC or sh-circ, were subcutaneously injected into the mice. The tumor volume was observed and recorded weekly using a vernier caliper. After 4 weeks, the mice were sacrificed, and the tumors were dissected. The tumor from each mouse was imaged, and their weights were measured. This experiment was approved by the Ethics Committee of Wuhan Third Hospital. | ||||