m6A-centered Crosstalk Information
Mechanism of Crosstalk between m6A Modification and Epigenetic Regulation
| Crosstalk ID |
M6ACROT05814
|
[1] | |||
m6A modification
miR-20a-5p
miR-20a-5p
METTL3
Methylation
 : m6A sites
Direct
Enhancement
Non-coding RNA
miR-20a-5p
NFIC
lncRNA miRNA circRNA
|
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| m6A Modification: | |||||
|---|---|---|---|---|---|
| m6A Regulator | Methyltransferase-like 3 (METTL3) | WRITER | |||
| m6A Target | hsa-miR-20a-5p | ||||
| Epigenetic Regulation that have Cross-talk with This m6A Modification: | |||||
| Epigenetic Regulation Type | Non-coding RNA (ncRNA) | ||||
| Epigenetic Regulator | hsa-miR-20a-5p | microRNA | View Details | ||
| Regulated Target | Nuclear factor 1 C-type (NFIC) | View Details | |||
| Crosstalk Relationship | m6A → ncRNA | Enhancement | |||
| Crosstalk Mechanism | m6A regulators directly modulate the functionality of ncRNAs through specific targeting ncRNA | ||||
| Crosstalk Summary | METTL3 promoted m6A modification and the binding of DGCR8 to hsa-miR-20a-5p to further elevate the miR-20a-5p expression and inhibit Nuclear factor 1 C-type (NFIC) transcription, thus promoting EMT, invasion and migration. | ||||
| Cell Process | Cell invasion | ||||
| Cell migration | |||||
| Epithelial-mesenchymal transition | |||||
In-vitro Model |
Eca-109 | Esophageal squamous cell carcinoma | Homo sapiens | CVCL_6898 | |
|
TE1
|
N.A. | Mus musculus | CVCL_C6K3 | ||
| In-vivo Model | Male nude mice (age: 4-6 weeks, weight: 22-22 g) provided by the Southern Medical University, were housed in specific pathogen environment at 22-24°C and 50-60% humility under a 12-h light/dark cycle, with ad libitum access to food and water. The nude mice were classified into 3 groups with 18 mice per group: the control group, the sh-NC group, and the sh-METTL3 group. Well-grown ECA109 cells, sh-METTL3-transfected ECA109 cells, sh-NC-transfected ECA109 cells were re-suspended in phosphate buffer saline (PBS) solution and then subcutaneously injected (1 × 107cells/mouse) into the right abdomen (N = 6) or injected into mice via the caudal veins (N = 6). For the subcutaneously injected mice, the tumor volume was evaluated every 7 days according to the following formula: V = ab2/2 (a: the longest diameter of the tumor; b: the shortest diameter of the tumor). After 30 days, the subcutaneously injected mice were euthanatized using an intraperitoneal injection of 1% pentobarbital (200 mg/kg). | ||||