m6A-centered Crosstalk Information
Mechanism of Crosstalk between m6A Modification and Epigenetic Regulation
| Crosstalk ID |
M6ACROT05788
|
[1] | |||
m6A modification
MIR320A
MIR320A
METTL3
Methylation
 : m6A sites
Direct
Inhibition
Non-coding RNA
MIR320A
Runx2
lncRNA miRNA circRNA
|
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| m6A Modification: | |||||
|---|---|---|---|---|---|
| m6A Regulator | Methyltransferase-like 3 (METTL3) | WRITER | |||
| m6A Target | hsa-mir-320a | ||||
| Epigenetic Regulation that have Cross-talk with This m6A Modification: | |||||
| Epigenetic Regulation Type | Non-coding RNA (ncRNA) | ||||
| Epigenetic Regulator | hsa-mir-320a | microRNA | View Details | ||
| Regulated Target | Runt-related transcription factor 2 (Runx2) | View Details | |||
| Crosstalk Relationship | m6A → ncRNA | Inhibition | |||
| Crosstalk Mechanism | m6A regulators directly modulate the functionality of ncRNAs through specific targeting ncRNA | ||||
| Crosstalk Summary | METTL3 enhanced, whereas METTL3 silence or knockout suppressed, the m6A methylations of Runt-related transcription factor 2 (Runx2) and hsa-mir-320a. | ||||
In-vitro Model |
BMSCs (BMSCs were obtained from the femurs and tibias of 2-3-week-old Sprague-Dawley male rats (Animal Center of Sun Yat-sen University)) | ||||
| In-vivo Model | We first confirmed that the expression levels of osteoblast differentiation markers BGLAP, BMP2, Col1a1, and alkaline phosphatase (ALP) were lower in osteoporosis patients and OVX mice than the normal levels in their corresponding control groups | ||||