m6A-centered Crosstalk Information
Mechanism of Crosstalk between m6A Modification and Epigenetic Regulation
| Crosstalk ID |
M6ACROT05742
|
[1] | |||
m6A modification
HS3ST3B1-IT1
HS3ST3B1-IT1
ALKBH5
Demethylation
 : m6A sites
Direct
Enhancement
Non-coding RNA
HS3ST3B1-IT1
HS3ST3B1
lncRNA miRNA circRNA
|
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| m6A Modification: | |||||
|---|---|---|---|---|---|
| m6A Regulator | RNA demethylase ALKBH5 (ALKBH5) | ERASER | |||
| m6A Target | Heparan Sulfate-Glucosamine 3-Sulfotransferase 3B1 Intronic Transcript 1 (HS3ST3B1-IT1) | ||||
| Epigenetic Regulation that have Cross-talk with This m6A Modification: | |||||
| Epigenetic Regulation Type | Non-coding RNA (ncRNA) | ||||
| Epigenetic Regulator | Heparan Sulfate-Glucosamine 3-Sulfotransferase 3B1 Intronic Transcript 1 (HS3ST3B1-IT1) | LncRNA | View Details | ||
| Regulated Target | Heparan sulfate-glucosamine 3-sulfotransferase 3B1 (HS3ST3B1) | View Details | |||
| Crosstalk Relationship | m6A → ncRNA | Enhancement | |||
| Crosstalk Mechanism | m6A regulators directly modulate the functionality of ncRNAs through specific targeting ncRNA | ||||
| Crosstalk Summary | ALKBH5-mediated m6A demethylation of Heparan Sulfate-Glucosamine 3-Sulfotransferase 3B1 Intronic Transcript 1 (Heparan sulfate-glucosamine 3-sulfotransferase 3B1 (HS3ST3B1)-IT1) prevents osteoarthritis progression | ||||
| Responsed Disease | Osteoarthritis | ICD-11: FA05 | |||
| Cell Process | mRNA stability | ||||
| In-vivo Model | 8-week-old male C57BL/6 mice were randomly divided into three groups: Ctrl+AAV-NC group (n=7), MIA+AAV-NC group (n=7), and MIA+ AAV-IT1 group (n=7). For the induction of OA, mice were given an intra-articular injection of MIA (Sigma-Aldrich, St. Louis, MO, USA) in the knee. Control mice were given normal saline in the same volume. One week later, 20 μL of AAV-HS3ST3B1-IT1 or AAV-NC (GeneChem, Shanghai, China) were injected into the knee joints of mice. Treatments were administered once per week for 3 consecutive weeks. Six weeks later, the mice were euthanized, and the knee joints were collected and store at -80°C. | ||||