m6A-centered Crosstalk Information
Mechanism of Crosstalk between m6A Modification and Epigenetic Regulation
| Crosstalk ID |
M6ACROT05653
|
[1] | |||
m6A modification
MALAT1
MALAT1
CYFIP2
 : m6A sites
Direct
Enhancement
Non-coding RNA
Malat1
Regulated Target
lncRNA miRNA circRNA
|
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| m6A Modification: | |||||
|---|---|---|---|---|---|
| m6A Regulator | Cytoplasmic FMR1-interacting protein 2 (CYFIP2) | READER | |||
| m6A Target | Metastasis associated lung adenocarcinoma transcript 1 (MALAT1) | ||||
| Epigenetic Regulation that have Cross-talk with This m6A Modification: | |||||
| Epigenetic Regulation Type | Non-coding RNA (ncRNA) | ||||
| Epigenetic Regulator | Metastasis associated lung adenocarcinoma transcript 1 (MALAT1) | LncRNA | View Details | ||
| Crosstalk Relationship | m6A → ncRNA | Enhancement | |||
| Crosstalk Mechanism | m6A regulators directly modulate the functionality of ncRNAs through specific targeting ncRNA | ||||
| Crosstalk Summary | RNA immunoprecipitation and mass spectrometry revealed 12 new synapse-specific learning-induced m6A readers in the mPFC of male C57/BL6 mice, with m6A-modified Metastasis associated lung adenocarcinoma transcript 1 (MALAT1) binding to a subset of these, including CYFIP2 and DPYSL2. In addition, a cell type- and synapse-specific, and state-dependent, reduction of m6A on Malat1 impairs fear-extinction memory; an effect that likely occurs through a disruption in the interaction between Malat1 and DPYSL2 and an associated decrease in dendritic spine formation. | ||||
| In-vivo Model | Adult, male C57BL/6J mice (9-10 weeks old, 20-25 g) supplied from the Animal Resources Center were used for experiments. Mice were housed 4 animals per cage on a 12 h light:dark cycle (lights on 0700 h) in a humidity- and temperature-controlled vivarium, with rodent chow and water provided ad libitum. | ||||